Effect of Dietary L-ascorbic Acid (L-AA) on Production Performance, Egg Quality Traits and Fertility in Japanese Quail (Coturnix japonica) at Low Ambient Temperature

  • Shit, N. (Avian Physiology and Reproduction Division, Central Avian Research Institute) ;
  • Singh, R.P. (Avian Physiology and Reproduction Division, Central Avian Research Institute) ;
  • Sastry, K.V.H. (Avian Physiology and Reproduction Division, Central Avian Research Institute) ;
  • Agarwal, R. (Avian Physiology and Reproduction Division, Central Avian Research Institute) ;
  • Singh, R. (Avian Physiology and Reproduction Division, Central Avian Research Institute) ;
  • Pandey, N.K. (Avian Physiology and Reproduction Division, Central Avian Research Institute) ;
  • Mohan, J. (Avian Physiology and Reproduction Division, Central Avian Research Institute)
  • Received : 2011.07.29
  • Accepted : 2011.10.28
  • Published : 2012.07.01


Environmental stress boosts the levels of stress hormones and accelerates energy expenditure which subsequently imbalance the body's homeostasis. L-ascorbic acid (L-AA) has been recognized to mitigate the negative impact of environmental stress on production performances in birds. The present investigation was carried out to elucidate the effect of different dietary levels of L-AA on production performance, egg quality traits and fertility in Japanese quail at low ambient temperature. Sixty matured females (15 wks) were equally divided into three groups (20/group) based on the different dietary levels of L-AA (0, 250 and 500 ppm) and coupled with an equal number of males (1:1) obtained from the same hatch. They were managed in uniform husbandry conditions without restriction of feed and water at 14 h photo-schedule. Except for feed efficiency, body weight change, feed consumption and hen-day egg production were recorded highest in 500 ppm L-AA supplemented groups. Among the all egg quality traits studied, only specific gravity, shell weight and thickness differed significantly (p<0.05) in the present study. Fertility was improved significantly ($p{\leq}0.01$) to a dose dependent manner of L-AA. The findings of the present study concluded that dietary L-AA can be a caring management practice at least in part to alleviate the adverse effect of cold induced stress on production performance in Japanese quail.


Cold Stress;Egg Quality;Fertility;Japanese Quail


  1. Ali, A., M. H. Saki Masud, Z. R. Pouya, Z. Khalil and M. R. H. Hamid. 2010. Can vitamin C elevate laying hen performance, egg and plasma characteristics under normal environmental temperature? Italian J. Anim. Sci. 2010; 9:e60.
  2. Arad, Z. and J. Marder. 1982. Comparison of the productive performances of the Sinai Bedouin fowl, the white leghorn and their crossbreeds: Study under natural desert conditions. Br. Poult. Sci. 23:33-45.
  3. Bains, B. S. 1996. The role of vitamin C in stress management. Misset World Poult. 12:4-38.
  4. Bollengier-Lee, S., P. E. V. Williams and C. C. Whitehead. 1999. Optimal dietary concentration of vitamin for alleviating the effect of heat stress on egg production in laying hens. Br. Poult. Sci. 40:102-107.
  5. Cheng, T. K., C. N. Coon and M. L. Hamre. 1990. Effct of environmental stress on the ascorbic acid requirement of laying hens. Poult. Sci. 69:774-780.
  6. Ciftici, M., O. N. Ertas and T. Guler. 2005. Effects of vitamin E and vitamin C dietary supplementation on egg production and egg quality of laying hens exposed to chronic heat stress. Rev. Med. Vet. (Toulouse) 156:107-111.
  7. Donkoh, A. 1989. Ambient temperature: a factor affecting performance and physiological response of broiler chickens. Int. J. Biometeorol. 33:259-265.
  8. Dorr, P. and S. L. Balloun. 1976. Effect of dietary vitamin A, ascorbic acid and their interaction on turkey bone mineralization. Br. Poult. Sci. 17:581-599.
  9. Dreiling, E. C., F. S. Carman and D. E. Brown. 1991. Maternal endocrine and fetal metabolic responses to heat stress. J. Dairy Sci. 74:312-327.
  10. Duncan, D. B. 1955. Multiple ranges and multiple F test. Biometrics 11:1-24.
  11. Eisen, E. J., B. B. Bohren and H. E. McKean. 1962. The haugh unit as a measure of egg albumen quality. Poult. Sci. 41:1461-1468.
  12. El-Boushy, A. R., P. C. M. Simons and G. Wiertz. 1988. Structure and ultra structure of the hen's egg shell as influenced by environmental temperature, humidity and vitamin C additions. Poult. Sci. 47:456-467.
  13. Ensminger, M. E., J. E. Oldfield and W. W. Heinemann. 1990. Feeds and nutrition. pp. 593-596. The Ensminger Publishing Company, USA.
  14. Gumuslu, S., A. Y. Gocmen and S. B. Sarykcioglu. 2002. Paraoxonase (PON1) activity in serum of rats stressed by cold: Effects of vitamin E supplementation. Proceedings of 17th National Biochemistry Congress. pp. 74:302. Ankara, Turkey.
  15. Hempe, J. M., R. C. Lauxen and J. E. Savage. 1988. Rapid determination of egg weight and specific gravity using a computerized data collection system. Poult. Sci. 67:902-907.
  16. Keshavarz, K. 1996. The effect of different levels of vitamin C and cholecalsiferol with adequate or marginal levels of dietary calcium on performance and egg shell quality of laying hens. Poult. Sci. 75:1227-1235.
  17. Klasing, K. C. 1998. Comparative avian nutrition, pp. 277-299. Cambridge University Press, London.
  18. Kontecka, H., J. Ksiązkiewicz, S. Nowaczewski, T. Kisiel and S. Krystianiak. 2001. Effect of feed supplementation with vitamin C on reproduction results in Pekin ducks. Proceedings of 9th International symposium on current problems of breeding, health and production of poultry. pp. 6-7 July 2001. Ceske Budejovice, Czech Republic.
  19. Kucuk, O., N. Sahin, K. Sahin, M. F. Gursu, M. Ozcelik and M. Issi. 2003. Egg production, egg quality, and lipid peroxidation status in laying hens maintained at a low ambient temperature ($6^{\circ}C$) and fed a vitamin C and vitamin E-supplemented diet. Vet. Med. Czech 48:33-40.
  20. Kutlu, H. R. and J. M. Forbes. 1993. Changes in growth and blood parameters in heat-stressed broiler chicks in response to dietary ascorbic acid. Livest. Prod. Sci. 36:335-350.
  21. Leeson, S. 2001. Feeding programs for laying hens. Vol. PO49. American Soybean Association Technical Bulletin.
  22. McDowell, L. R. 1989. Vitamins in animal nutrition: Comparative aspects to human nutrition. pp. 93-131. (Ed. L. R. Mc Dowell). Academic Press, London.
  23. Mowat, D. N. 1994. Organic chromium: A new nutrient for stressed animals. pp. 275-282. Proceedings of Alltech's 10th Annual symposium (Ed. T. P. Lyons and K. A. Jacques). Nottingham University Press, Nottingham, UK.
  24. Njoku, P. C. and A. O. U. Nwazota. 1989. Effectofdietaryinclusion of ascorbic acid and palm oil on the performance of laying hens in hot tropical environment. Br. Poult. Sci. 30:831-840.
  25. Nowaczewski, S. and H. Kontecka. 2005. Effect of dietary vitamin C supplement on reproductive performance of aviary phesants. Czech J. Anim. Sci. 50:208-212.
  26. Orban, J. I., D. A. Ronald, Jr. K. Cummins and R. T. Lovell. 1993. Influence of large doses of ascorbic acid on performance, plasma calcium, bone characteristics and egg shell quality in broiler and leghorn hens. Poult. Sci. 72:691-700.
  27. Peebles, E. D. and J. Brake. 1985. Relationship of dietary ascorbic acid to broiler breeder performance. Poult. Sci. 64:2041-2048.
  28. Sahin, K., O. Kucuk and N. Sahin. 2001a. Effects of dietary chromium picolinate supplementation on performance and plasma concentrations of insulin and corticosterone in laying hens under low ambient temperature. J. Anim. Physiol. Anim. Nutr. 85:142-147.
  29. Sahin, N. and K. Sahin. 2001b. Optimal dietary concentrations of vitamin c and chromium picolinate for alleviating the effect of low ambient temperature ($6.2^{\circ}C$) on egg production, some egg characteristics and nutrient digestibility in laying hens. Vet. Med. Czech. 46:229-236.
  30. Sahin, K., O. Kucuk, N. Sahin and M. Sari. 2002. Effects of vitamin C and vitamin E on lipid peroxidation status, some serum hormone, metabolite, and mineral concentrations of Japanese quails reared under heat stress ($34^{\circ}C$). Int. J. Vitam. Nutr. Res. 72:91-100.
  31. Siegel, H. S. 1995. Stress, strains and resistance. Br. Poult. Sci. 36: 3-24.
  32. Snedecor, G. W. and W. G. Cochran. 1994. Statistical Methods, 8th ed. Oxford and IBH Publication Kolkata, India.
  33. Spinu, M. and A. A. Degen. 1993. Effect of cold stress on performance and immune responses of Bedouin and White Leghorn hens. Br. Poult. Sci. 34:177-185.
  34. Torgowski, J. and H. Kontecka. 1998. WpIyw dodatku wita-miny C i zelaza do paszy na wskazniki produkcyjne i hematologiczne bazantow lownych (Phasianus colchi-cus). Rocz. AR Pozn. 302, Zoot. 50:235-242.
  35. Weber, P., A. Bendich and W. Schalch. 1996. Ascorbic acid and human health-a review of recent data relevant to human requirements. Int. J. Vitam. Nutr. Res. 66:19-30.

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