Quantitative Determination of Fe-oxidation State by Electron Energy Loss Spectroscopy (EELS)

전자에너지 손실분광 분석법을 이용한 정량적 철산화수 측정

  • Received : 2012.03.29
  • Accepted : 2012.04.20
  • Published : 2012.04.28


The consequences of microbe-mineral interaction often resulted in the chemical, structural modification, or both in the biologically induced mineral. It is inevitable to utilize the high powered resolution of electron microscopy to investigate the mechanism of biogenic mineral transformation at nano-scale. The applications of transmission electron microscopy (TEM) capable of electron energy loss spectroscopy (EELS) to the study of microbe-mineral interaction were demonstrated for two examples: 1) biogenic illite formation associated with structural Fe(III) reduction in nontronite by Fereducing bacteria; 2) siderite phase formation induced by microbial Fe(III) reduction in magnetite. In particular, quantification of the changes in Fe-oxidation state at nanoscale is essential to understand the dynamic modification of minerals resulted from microbial Fe reduction. The procedure of EELS acquisition and advantages of EELS techniques were discussed.


TEM;EELS;Fe-reduction;Mineral transformation


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  1. Electron Energy Loss Spectroscopy (EELS) Application to Mineral Formation vol.29, pp.2, 2016,


Supported by : National Research Foundation of Korea (NRF)