Preparation of Nano-emulsion Containing Polygonum aviculare Extract for Enhanced Transdermal Delivery

피부 흡수 증진을 위한 마디풀 추출물 함유 나노에멀젼 제조에 관한 연구

  • Lim, Myoung Sun (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Min A (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D Center, Seoul National University of Science and Technology)
  • 임명선 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 박민아 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소)
  • Published : 2012.04.10

Abstract

In this study, nano-emulsion containing ethyl acetate fraction of Polygonum aviculare (P. aviculare) extract was prepared and skin permeability of the nano-emulsion was evaluated. Nano-emulsion was prepared using homogenizer and microfluidizer by the high-energy method. The droplet size and loading efficiency of nano-emulsion containing ethyl acetate fraction of P. aviculare extract were determined. The mean droplet size was 238 nm and the loading efficiency was more than 98%. The size distribution of nano-emulsion was a monodispersed form and nano-emulsion was more stable than that of using typical emulsion without using the microfluidizer. The in vitro skin permeation study of nano-emulsion containing ethyl acetate fraction of P. aviculare extract was carried out using Franz diffusion cells. Compared to 1,3-butylene glycol, nano-emulsion showed greater values of cumulative permeation of ethyl acetate fraction from P. aviculare extract. These results indicate that the stability and skin permeability of nano-emulsion containing ethyl acetate fraction of P. aviculare exerting anti-oxidative and anti-aging activities were enhanced.

References

  1. B. R. Won. M. K. Kang, and S. N. Park, J. Soc. Comet. Scientist, Korea., 35, 79 (2009).
  2. L. Houillot, J. Nicolas, M. Save, and B. Charleus, Langmuir, 21, 6726 (2005). https://doi.org/10.1021/la050495b
  3. A. Musyanovych, V. Mailander, and K. Landfester, Biomacromolecules, 6, 1824 (2005). https://doi.org/10.1021/bm050084+
  4. W. Seifriz, Science, 87, 212 (1938). https://doi.org/10.1126/science.87.2253.212
  5. M. Cajkovac, L. Oremovic, and V. Cajkovac, Pharmazie, 47, 39 (1992).
  6. M. Sznitowska, K. Zurowska-Pryczkowska, S. Janicki, and T. Jarvinen, Int. J. Pharm., 184, 115 (1999). https://doi.org/10.1016/S0378-5173(99)00106-4
  7. P. F. Yue, H. L. Yuan, W. F. Zhu, L. B. Cong, H. Xie, Z. G. Liu, L. J. Wang, and X. H. Xiao, Biol. Pharm. Bull., 31, 45 (2008). https://doi.org/10.1248/bpb.31.45
  8. R. Barnadas-Rodriguez and M. Sabes, Int. J. Pharm., 213, 175 (2001). https://doi.org/10.1016/S0378-5173(00)00661-X
  9. A. Towata, M. Sivakumar, K. Yasui, T. Tuziuti, T. Kozuka, and Y. Lida, Ultrason Sonochem, 14, 705 (2007). https://doi.org/10.1016/j.ultsonch.2007.01.001
  10. P. Izquierdo, J. Feng, J. Esquena, T. F. Tadros, J. C. Dederen, M. J. Garcia, M. Azemar, and C. Solans, J. Colloid Interface Sci., 285, 388 (2005). https://doi.org/10.1016/j.jcis.2004.10.047
  11. J. B. Tagne, S. Kakumanu, and R. J. Nicolosi, Mol. Pharm., 5, 1055 (2008). https://doi.org/10.1021/mp8000556
  12. S. B. Calderilla-Fajardo, J. Cazares-Delgadillo, R. Villalobos-Garcia, D. Quintanar-Guerrero, A. Ganem- Quintanar, and R. Robles, Drug Dev. Ind. Pharm., 32, 107 (2006). https://doi.org/10.1080/03639040500388540
  13. C. Solans, J. Esquena, A. Forgiarini, D. Morales, N. Uso'n, and P. Izquierdo, Nano emulsions: formation and properties: surfactants in solution: fundamentals and applications, Surfactant Science Series, eds. D. Shah, B. Moudgil, K. L. Mittal, Marcel Dekker, New York, 525 (2002).
  14. E. D. Sudol and M. S. El-Aasser, In emulsion polymerization and emulsion polymers, P. A. Lovell and M. S. El-Aasser, Eds., John Wiley & Sons, Ltd., Chichester, United Kingdom, 700 (1997).
  15. L. Marszall, In nonionic surfactants: surfactant science series, ed. M. J. Shick, Marcel Dekker, New York, 23, 493 (1987).
  16. K. Shinoda and H. J. Saito, Colloid Interface Sci., 26, 70 (1968). https://doi.org/10.1016/0021-9797(68)90273-7
  17. F. Q. Xu, H. B. Liu, J. G. Luo, J. L. Zhang, and H. S. Guan, Periodical of Ocean University of China, 3 (2010)
  18. F. Q. Xu, H. S. Guan, G. Q. Li, and H. B. Liu, Chromatograpia, 69, 1251 (2009). https://doi.org/10.1365/s10337-009-1088-x
  19. C. Y. Hsu, Biol Res., 39, 281 (2006).
  20. H. S. Kim, Kor. J. Mycol., 8, 1 (1980).
  21. S. N. Park, S. Y. Kim, M. J. Kim, G. N. Lim, M. S. Lim, H. J. Lee, M. A. Park, and J. E. Kim, Cellular Protective Effect of Polygonum aviculare Extract and Preparation of Liposome Containing It, IFSCC conference, Bangkok, (2011).
  22. W. G. Cho, J. Korean Oil Chemists' Soc., 24, 410 (2007).
  23. S. S. Baik. J. Soc. Comet. Scientist Korea., 19, 127 (1993).
  24. K. Y. Kyoung, U. K. Jee, and W. G. Cho, J. Kor. Pharm. Sci., 37, 85 (2007)
  25. H. S. Kang, S. S, Kwon, B. H. Kim, B. R. Lee, K. H. Kang, J. E. Hong, S. H. Han, and I. S. Chang, J. Ind. Eng. Chem., 8, 348 (2002).
  26. W. G. Cho, J. Soc. Comet. Scientist, Korea., 37, 1 (2011).