Organic Acid and Enzyme Pretreatment of Laminaria japonica for Bio-ethanol Production

유기산 및 효소적 전처리를 이용한 다시마에서 바이오 에탄올 생산

  • Lee, Sung-Mok (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
  • 이성목 (신라대학교 의생명과학대 생명공학과) ;
  • 이재화 (신라대학교 의생명과학대 생명공학과)
  • Published : 2012.04.10


We investigated for the production of biological bio-ethanol from Laminaria japonica using the hydrolysis reaction of enzymes and organic acids and the polysaccharide content was also analyzed. The composition of the polysaccharide was characterized as 65.99% alginate, 6.24% laminaran and 27.77% mannitol. The optimum concentration for reducing the sugar conversion by Laminaria japonica was found to be 1.874 g/L at an acetic acid concentration of 1.5%, $121^{\circ}C$ for 60 min, and for an ascorbic acid of 2.0%, 4.291 g/L was produced in the same condition. The enzyme hydrolysis such as alginate lyase and laminarinase contained the maximum 2.219 g/L reducing sugar. In the result of ethanol fermentation using hydrolysate of Laminaria japonica, the organic acid treatment showed a high of reducing sugar yield, but decreased the ethanol yield, and then the maximum ethanol production obtained was 1.26 g/L using the mixed treated of enzyme.


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