Characterization of Erwinia tasmaniensis Isolated from Nuruk Producing Alginate Lyase

누룩으로부터 분리한 알긴산 분해 효소 생산 균주인 Erwinia tasmaniensis의 특성

  • Kim, Hyun Ji (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Lee, Sung-Mok (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University) ;
  • Kim, Sung-Koo (Department of Biotechnology, Pukyong National University) ;
  • Lee, Jae-Hwa (Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University)
  • 김현지 (신라대학교 의생명과학대학 생명공학과) ;
  • 이성목 (신라대학교 의생명과학대학 생명공학과) ;
  • 김성구 (부경대학교 수산과학대학 생물공학과) ;
  • 이재화 (신라대학교 의생명과학대학 생명공학과)
  • Published : 2012.02.10

Abstract

Oligosaccharides production showed various biological activities in vivo like functional foods and industrial materials utilized available within many practical applications which have obtained from the degradation of alginate. Alginate is rich in the main component of seaweeds especially the brown algae. We investigated what degrading alginate from seaweeds to make alginate oligosaccharides can utilize in various fields using enzyme secreting Erwinia tasmaniensis. In this study, we observed an optimal culture condition of E. tasmaniensis, and characteristics of alginate lyase secreting E. tasmaniensis. These bacteria, E. tasmaniensis, were isolated from Nuruk. In this case, a suitable growth factor for E. tasmaniensis was culture it for 36 h in broth media on concentration of 1.0% (w/v) alginate. The enzyme showed the highest level of alginate lyase activity when cultured on broth media containing 1.0% (w/v) sodium alginate for 72 h. Optimal condition of pH, temperature and duration time for alginate lyase activity were found to be pH 6.0, $20^{\circ}C$ and 60 min, respectively.

Keywords

alginate;alginate lyase;Erwinia tasmaniensis;oligosaccharide

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