G Protein Mediated Hatching Regulation in the Mouse Embryo

  • Cheon, Yong-Pil (School of Biosciences and Chemistry, College of Natural Sciences, Sungshin Women's University)
  • Received : 2012.02.29
  • Accepted : 2012.03.21
  • Published : 2012.03.31

Abstract

Hatching occurred in the time dependent manners and strictly controlled. Although, the hatching processes are under the control of muti-embryotrophic factors and the expressed G proteins of cell generate integrated activation, the knowledge which GPCRs are expressed during hatching stage embryos are very limited. In the present study, which G proteins are involved was examined during blastocyst development to the hatching stage. The early-, expanded-, and lobe-stage blastocysts were treated with various $G_{\alpha}$ activators and H series inhibitors, and examined developmental patterns. Pertusis toxin (PTX) improved the hatching rate of the early-stage blastocyst and lobe-formed embryos. Cholera toxin (CTX) suppressed the hatching of the early-stage blastocyst and expanded embryos. The effects of toxins on hatching and embryo development were changed by the H7 and H8. These results mean that PTX mediated GPCRs activation is signaling generator in the nick or pore formation in the ZP. In addition, PTX mediated GPCR activation induces the locomotion of trophectoderm for the escaping. CTX mediate GPCRs activation is the cause of suppression of hatching processes. Based on these data, it is suggested that various GPCRs are expressed in the periimplantation stage embryos and the integration of the multiple signals decoding of various signals in a spatial and temporal manner regulate the hatching process.

Acknowledgement

Supported by : Sungshin Women's University

References

  1. Biggers JD, Whitten WK, Whittingham DB (1971) The culture of mouse embryos in vitro. In: Daniel JC Jr, ed. Methods in Mammalian Embryology, San Francisco, pp.86-116, Freeman.
  2. Cheon YP, Gye MC, Kim CH, Kang BM, Chang YS, Kim SR, Kim MK (1999) Role of actin filaments in the hatching process of mouse blastocyst. Zygote 7: 123- 129. https://doi.org/10.1017/S0967199499000477
  3. Cheon YP (2005) Concanavalin A mediated calcium changes on expansion and hatching of the mouse blastocyst. Kor J Fertil Steril 32:337-346.
  4. Cheon YP (2006) PAF regulate blastocyst development to hatching stage through PKC activity in the mouse. Reprod Dev Biol 30:75-79.
  5. Cheon YP (2010) Effects of EGF and PAF on the hatching and implantation of peri-implantation stage embryos. Dev Reprod 14:27-34.
  6. Engh RA, Girod A, Kinzel V, Huber R, Bossemeyer D (1996) Crystal structures of catalytic subunit of cAMPdependent protein kinase in complex with isoquinolinesulfonyl protein kinse inhibitors H7, H8, and H89. Structural implications for selectivity. J Biol Chem 271:26157-26164. https://doi.org/10.1074/jbc.271.42.26157
  7. Fujino H, Regan JW (2006) EP(4) prostanoid receptor coupling to a pertusis toxin-sensitive inhibitory G protein. Mol Pharmacol 69:5-10.
  8. Gonzales DS, Bavister BD, Mese SA (2001) In utero and in vitro proteinase activity during the Mesocricetus auratus embryo zona escape time window. Biol Reprod 64: 222-230. https://doi.org/10.1095/biolreprod64.1.222
  9. Hur EM, Kim KT (2002) G protein-coupled receptor signaling and cross-talk: achieving rapidity and specificity. Cell Signal 14:397-405. https://doi.org/10.1016/S0898-6568(01)00258-3
  10. Kane MT, Morgan PM, Coonan C (1997) Peptide growth factors and preimplantation development. Hum Reprod Update 3:137-157. https://doi.org/10.1093/humupd/3.2.137
  11. Kawagishi R, Tahara M, Sawada K, Ikebuchi Y, Morishige K, Sakata M, Tasaka K, Murata Y (2004a) Rho-kinase is involved in mouse blastocyst cavity formation. Biochem Biophys Res Commun 319:643-648. https://doi.org/10.1016/j.bbrc.2004.05.040
  12. Kawagishi R, Tahara M, Sawada K, Morishige K, Sakata M, Tasaka K, Murata Y (2004b) Na+/H+ exchanger-3 is involved in mouse blastocyst formation. J Exp Zoolog A Comp Exp Biol 301: 767-775.
  13. Kidder GM, Watson AJ (2005) Roles of Na,K-ATPase in early development and trophectoderm differentiation. Semin Nephrol 25: 352-355. https://doi.org/10.1016/j.semnephrol.2005.03.011
  14. Kurotaki Y, Hatta K, Nakao K, Nabeshima Y, Fujimori T (2007) Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape. Science 316:719-723. https://doi.org/10.1126/science.1138591
  15. Oda H, Naganuma T, Murayama T, Nomura Y (1999) Inhibition of noradrenalin release from PC12 cells by the long-term treatment with cholera toxin. Neurochem Int 34:157-165. https://doi.org/10.1016/S0197-0186(98)00084-9
  16. Petersen CG, Mauri AL, Baruffi RL, Oliveira JB, Massaro FC, Elder K, Franco Jr JG (2005) Implantation failures: success of assisted hatching with quarter-laser zona thinning. Reprod Biomed Online 10:224-229. https://doi.org/10.1016/S1472-6483(10)60944-3
  17. Prezeau L, Rives ML, Comps-Agrar L, Maurel D, Kniazeff J, Pin JP (2010) Functional crosstalk between GPCRs: with or without oligomerization. Curr Opin Pharmacol 10:6-13. https://doi.org/10.1016/j.coph.2009.10.009
  18. Pribenszky C, Cornea M, Jando T, Losonczi E, Lang Z (2011) 100 the dynamics of contractions of mouse blastocysts in vitro is typical for embryo quality and may predict hatching-a time-lapse-based analysis. Reprod Fertil Dev 24:162-163.
  19. Riley JK, Carayannopoulos MO, Wyman AH, Chi M, Ratajczak CK, Moley KH (2005) The PI3K/Akt pathway is present and functional in the preimplantation mouse embryo. Dev Biol 284:377-386. https://doi.org/10.1016/j.ydbio.2005.05.033
  20. Seshagiri PB, Roy SS, Sireesha G, Pao RP (2009) Cellular and molecular regulation of mammalian blastocyst hatching. J Reprod Immunol 83:79-84. https://doi.org/10.1016/j.jri.2009.06.264
  21. Seshagiri PB, Mishra A, Ramesh G, Rao RP (2002) Regulation of peri-attachment embryo development in the golden hamster: role of growth factors. J Reprod Immunol 53:203-213. https://doi.org/10.1016/S0165-0378(01)00086-9
  22. Sireesha GV, Mason RW, Hassanein M, Tonack S, Navarrete Santos A, Fischer B, Seshagiri PB (2008) Role of cathepsins in blastocyst hatching in the golden hamster. Mol Hum Reprod 14:337-346. https://doi.org/10.1093/molehr/gan026
  23. Strumpf Dm, Mao CA, Yamanaka Y, Ralston A, Chawengsaksophak K, Beck F, Rossant J (2005) Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development 132:2093-2102. https://doi.org/10.1242/dev.01801
  24. Tadevosyan A, Vaniotis G, Allen BG, H'ebert TE, Nattel S (2012). G protein-coupled receptor signaling in the cardiac nuclear membrane: evidence and possible roles in physiological and pathophysiological function. J Physiol 590:1313-1330. https://doi.org/10.1113/jphysiol.2011.222794
  25. Tsubaki M, Matsuoka H, Yamamoto C, Kato C, Ogaki M, Satou T, Itoh T, Kusunoki T, Tanimori Y, Nishida S (2007) The protein kinase C inhibitor, H7, inhibits tumor cell invasion and metastasis in mouse melanoma via suppression of ERT1/2. Clin Exp Metastasis 24: 431-438. https://doi.org/10.1007/s10585-007-9080-z
  26. Wassarman PM (2008) Zona pellucida glycoproteins. J Biol Chem 283:24285-24289. https://doi.org/10.1074/jbc.R800027200
  27. Watson AJ, Bacroft LC (2001) Regulation of blastocyst formation. Front Biosci 6:D708-D730. https://doi.org/10.2741/Watson