Eryngium foetidum Suppresses Inflammatory Mediators Produced by Macrophages

  • Mekhora, Chusana (Institute of Nutrition, Mahidol University) ;
  • Muangnoi, Channarong (Institute of Nutrition, Mahidol University) ;
  • Chingsuwanrote, Pimjai (Institute of Nutrition, Mahidol University) ;
  • Dawilai, Suwitcha (Institute of Nutrition, Mahidol University) ;
  • Svasti, Saovaros (Institute of Molecular Biosciences, Mahidol University) ;
  • Chasri, Kaimuk (Faculty of Allied Health Science, Thammasart University) ;
  • Tuntipopipat, Siriporn (Institute of Nutrition, Mahidol University)
  • Published : 2012.02.29


Objective: This study assessed anti-inflammatory and antioxidant activities of $E.$ $foetidum$ leaf extract on LPS-activated murine macrophages. Methods: RAW264.7 cells were pretreated with or without $E.$ $foetidum$ extract for 1 h prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to iNOS, COX-2, TNF-${\alpha}$ and IL-6 gene expression. In addition, NO and intracellular ROS generation were determined by Griess method and fluorescence intensity and activation of MAPKs and $I{\kappa}B$ by Western blotting. Results: Prior treatment with $E.$ $foetidum$ leaf extract inhibited elevation of IL-6, TNF-${\alpha}$, iNOS and COX-2, together with their cognate mRNAs in a dose-dependent manner. NO and intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of JNK and p38 as well as $I{\kappa}B$. $E.$ $foetidum$ ethanol extract were shown to contain lutein, ${\beta}$-carotene, chlorogenic acid, kaempferol and caffeic acid, compounds known to exert these bioactive properties. Conclusions: $E.$ $foetidum$ leaf extract possesses suppressive effects against pro-inflammatory mediators. Thus, $E.$ $foetidum$ has a high potential to be used as a food supplement to reduce risk of cancer associated with inflammation.


E. foetidum;iNOS;COX 2;TNF ${\alpha}$;IL 6;inflammatory mediators


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