DOI QR코드

DOI QR Code

Expression of Tiam1 in Lung Cancer and its Clinical Significance

  • Wang, Hong-Ming (The First Affiliated Hospital of Zhengzhou University) ;
  • Wang, Jing (The First Affiliated Hospital of Zhengzhou University)
  • Published : 2012.02.29

Abstract

The aim of this study was to ana1yze T-cell lymphoma invasion and metastasis-inducing factor 1 (Tiam1) expression in 1ung cancer patients. A total of 204 patients with lung cancer tissue lesions were enrolled in the present study, along with 40 cases of normal lung tissue and 40 of normal fetal lung tissue. Tiam1 protein expression level was determined using intensity quantitative analysis, for comparison in lung cancer, metastatic, normal lung, and fetal lung tissue. The positive unit (PU) of Tiam1 was $13.5{\pm}5.42$ in lung cancer,$5.67{\pm}1.56$ in norma1 epithelial cells, and $5.89{\pm}1.45$ in fetal lung epithelial cells. The value in the lung cancer tissue was significantly higher than that in the normal lung tissue and the fetal lung tissue (P<0.01). The Tiam1 PU values with lymph node metastasis and without 1ymph node metastasis were $15.2{\pm}4.34$ and $12.5{\pm}4.23$, respectively, and the difference was statistically significant (P<0.05). The Tiam1 PU values in different tumor, nodes, metastasis (TNM) stages, III-IV period, and I-II phase were $14.7{\pm}4.14$ and $11.0{\pm}5.34$ (P<0.05). A correlation was found between Tiam1 expression and the age of patient, tumor size, tumor type, and tumor differentiation. Tiam1 protein expression in the lung tumor tissue is significantly higher than that in the normal lung tissue and fetal lung tissue. Tiam1 expression may be closely related to lung cancer development and metastasis.

Keywords

Lung cancer;tissue microarray;cancer deve1opment;Tiam 1

References

  1. Adams HC, Chen R, Liu Z, et al (2010). Regulation of breast cancer cell motility by T-cell lymphoma invasion and metastasis-inducing protein. Breast Cancer Res, 12, R69. https://doi.org/10.1186/bcr2637
  2. Buchanan FG, Elliot CM, Gibbs M, et al (2000). Translocation of the Rac1 guanine nucleotide exchange factor Tiam1 induced by platelet-derived growth factor and lysophosphatidic acid. J Biol Chem, 275, 9742-8. https://doi.org/10.1074/jbc.275.13.9742
  3. Chen B, Ding Y, Liu F, et al (2012). Tiam1, overexpressed in most malignancies, is a novel tumor biomarker. Mol Med Report, 5, 48-53.
  4. Ding Y, Chen B, Wang S, et al (2009). Overexpression of Tiam1 in hepatocellular carcinomas predicts poor prognosis of HCC patients. Int J Cancer, 124, 653-8. https://doi.org/10.1002/ijc.23954
  5. Engers R, Mueller M, Walter A, et al (2006). Prognostic relevance of Tiam1 protein expression in prostate carcinomas. Br J Cancer, 95, 1081-6. https://doi.org/10.1038/sj.bjc.6603385
  6. Ferrandina G, Martinelli E, Petrillo M, et al (2009). CD133 antigen expression in ovarian cancer. BMC Cancer, 9, 221. https://doi.org/10.1186/1471-2407-9-221
  7. Fleming IN, Gray A, Downes CP (2000). Regulation of the Rac1-specific exchange factor Tiam1 involves both phosphoinositide 3-kinase-dependent and -independent components. Biochem J, 351, 173-82. https://doi.org/10.1042/0264-6021:3510173
  8. Fritz G, Brachetti C, Bahlmann F, et al (2002). Rho GTPases in human breast tumours: expression and mutation analyses and correlation with clinical parameters. Br J Cancer, 87, 635-44. https://doi.org/10.1038/sj.bjc.6600510
  9. Hou M, Tan L, Wang X, et al (2004). Antisense Tiam1 downregu1ates the invasiveness of 95 D ce11s in vitro. Acta Biochim Biophys Sin (Shanghai), 36, 537-40. https://doi.org/10.1093/abbs/36.8.537
  10. Hsueh C, Lin JD, Yang CF, et al (2011). Prognostic significance of Tiam1 expression in papillary thyroid carcinoma. Virchows Arch, 459, 587-93. https://doi.org/10.1007/s00428-011-1164-0
  11. Li YM, Qi WJ, Shen H (2011). Quantitative analysis of Tiam1 expression in lung cancer and its clinical significance. Nan Fang Yi Ke Da Xue Xue Bao, 31, 1774-7.
  12. Liu L, Zhang Q, Zhang Y, et al (2006). Lentivirus-mediated silencing of Tiam1 gene influences multiple functions of a human colorectal cancer cell line. Neoplasia, 8, 917-24. https://doi.org/10.1593/neo.06364
  13. McHenry PR, Vargo-Gogola T (2010). Pleiotropic functions of Rho GTPase signaling: a Trojan horse or Achilles' heel for breast cancer treatment? Curr Drug Targets, 11, 1043-58. https://doi.org/10.2174/138945010792006852
  14. Minard ME, Kim LS, Price JE, et al (2004). The role of the guanine nucleotide exchange factor Tiam1 in cellular migration, invasion, adhesion and tumor progression. Breast Cancer Res Treat, 84, 21-32. https://doi.org/10.1023/B:BREA.0000018421.31632.e6
  15. Minard ME, Kim LS, Price JE, et al (2004). The role of the guanine nucleotide exchange factor Tiam1 in cellular migration, invasion, adhesion and tumor progression. Breast Cancer Res Treat, 84, 21-32. https://doi.org/10.1023/B:BREA.0000018421.31632.e6
  16. Sonoda Y, Sasaki K (2008). Surface morphology of the central macrophages of erythroblastic islets in the spleen of aged and pregnant mice: an immunohistochemical light microscopic study. Arch Histol Cytol, 71, 155-61 https://doi.org/10.1679/aohc.71.155
  17. Stebel A, Brachetti C, Kunkel M, et al (2009). Progression of breast tumors is accompanied by a decrease in expression of the Rho guanine exchange factor Tiam1. Oncol Rep, 21, 217-22.
  18. Walch A, Seidl S, Hermannstädter C, et al (2008). Combined analysis of Rac1, IQGAP1, Tiam1 and E-cadherin expression in gastric cancer. Mod Pathol, 21, 544-52. https://doi.org/10.1038/modpathol.2008.3
  19. Yang W, Lv S, Liu X, et al (2010). Up-regulation of Tiam1 and Rac1 correlates with poor prognosis in hepatocellular carcinoma. Jpn J Clin Oncol, 40, 1053-9. https://doi.org/10.1093/jjco/hyq086
  20. Zhao L, Liu Y, Sun X, et al (2011). Overexpression of T lymphoma invasion and metastasis 1 predict renal cell carcinoma metastasis and overall patient survival. J Cancer Res Clin Oncol, 137, 393-8. https://doi.org/10.1007/s00432-010-0895-7

Cited by

  1. Targeting Tumor Metastasis by Regulating Nm23 Gene Expression vol.13, pp.8, 2012, https://doi.org/10.7314/APJCP.2012.13.8.3539
  2. TIAM2 Enhances Non-small Cell Lung Cancer Cell Invasion and Motility vol.14, pp.11, 2013, https://doi.org/10.7314/APJCP.2013.14.11.6305
  3. miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9 vol.29, pp.5, 2013, https://doi.org/10.3892/or.2013.2300
  4. MicroRNA-377 Suppresses Cell Proliferation and Invasion by Inhibiting TIAM1 Expression in Hepatocellular Carcinoma vol.10, pp.3, 2015, https://doi.org/10.1371/journal.pone.0117714