- Volume 13 Issue 2
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CpG Island Methylation Profile of Estrogen Receptor Alpha in Iranian Females with Triple Negative or Non-triple Negative Breast Cancer: New Marker of Poor Prognosis
- Ramezani, Fatemeh (Department of Clinical Biochemistry and Nutrition, Faculty of Medicine, Urmia University of Medical Sciences) ;
- Salami, Siamak (Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences) ;
- Omrani, Mir Davood (Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences) ;
- Maleki, Davood (Department of Hematology and Oncology, Faculty of Medicine, Urmia University of Medical Sciences)
- Published : 2012.02.29
One decade early onset of the breast cancer in Iranian females was reported but the basis of the observed difference has remained unclear and difference in gene silencing by epigenetic processes is suggested. Hence, this study was sought to map the methylation status of estrogen receptor (ER) gene CpG islands and its impact on clinicopathological factors of triple negative and non-triple negative ductal cell carcinoma of the breast in Iranian females. Surgically resected formalin-fixed paraffin-embedded breast tissues from sixty Iranian women with confirmed invasive ductal carcinoma were assessed by methylation-specific PCR using primer sets encompassing some of the 29 CpGs across the ER gene CpG island. The estrogen and progesterone receptors, Her-
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