DOI QR코드

DOI QR Code

Analysis of Antibacterial Activity against Food Spoilage and Food-borne Pathogens and Cytotoxicity on Human Cancer Cell Lines of Extracts from Pericarp and Seed of Vitis coignetiea

머루 과피와 종자 추출물의 식품 위해성 세균에 대한 항균성 및 인체 암세포주에 대한 cytotoxicity 분석

  • Published : 2012.04.30

Abstract

In this study, antibacterial activity and cytotoxicity of the extracts from pericarp and seed of $Vitis$ $coignetiea$, which were extracted with 0.1% HCl-60% ethanol, were analyzed. The antibacterial activity of the extracts was determined by paper disc diffusion method against food spoilage and food-borne pathogens. The pericarp extract showed high antibacterial activity against $Bacillus$ $cereus$, $Escherichia$ $coli$ O157:H7, and $Pseudomonas$ $aeruginosa$, and the seed extract represented the high antibacterial activity against $B.$ $cereus$, $E.$ $coli$ O157:H7, and $Staphylococcus$ $aureus$. The cytotoxicity of the $Vitis$ $coignetiea$ extract against human cancer cells was determined using the MTT assay and SRB assay. The pericarp extract represented strong growth-inhibition activity against G361 and Hep3B cells and the seed extract greatly inhibited the growth of HeLa and G361 cells in the MTT assay. In addition, the pericarp extract displayed a high inhibition activity against the growth of AGS cells and the seed extract greatly inhibited the growth of HeLa, Hep3B, and MCF7 cells in the SRB assay. Especially, the cytotoxicities of the seed extract against HeLa were significantly higher than those of the extract against other cancer cells at all test concentrations. This study demonstrates that the extract from pericarp and seed of $Vitis$ $coignetiea$ possess high antibacterial activity and cytotoxicity.

Keywords

antibacterial activity;cytotoxicity;Vitis coignetiea

References

  1. Ikigai H, Nakae T, Hara Y, Shimamura T. 1993. Bactericidal catechins damage the lipid bilayer. Biochim Biophys Acta 1147(1):132-136 https://doi.org/10.1016/0005-2736(93)90323-R
  2. Jang M, Cai L, Udeani G, Slowing KV, Thomas CF, Beecher CWW, Fong SSD, Farnsworth NR, Douglas Kinghorn A, Mehta RG, Moon RC, Pezzuto JM. 1997. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275:218-220 https://doi.org/10.1126/science.275.5297.218
  3. Jeon TW, Cho YS, Lee SH, Cho SM, Cho HM, Jang KS, Park HJ. 2005. Studies on the biological activities and physicochemical characteristics of pigments extracted from Korean purple-fleshed potato. Korean J Food Sci Technol 37(2):247-254
  4. Jeon YH. 2008. Physiological activity of extracts of Rubus coreanum and isolation and identification of its flavonoid. M.A. Thesis. Kyungpook National University. Korea
  5. Jeon YH, Kim MH, Kim MR. 2008. Antioxidative, antimutagenic, and cytotoxic activities of ethanol extracts from Cornus officianalis. J Korean Soc Food Sci Nut 37(1):1-7 https://doi.org/10.3746/jkfn.2008.37.1.1
  6. Jeong SW. 2005. Antitumor and antioxidant activities of anthocyanins isolated from the seeds of Liriope platyphylla. M.A. Thesis. Catholic University of Daegu. Korea
  7. Jin YM, Nam SL, Ahn WS. 2002. Growth inhibition and induction of apoptosis in cervical cancer cell lines by green tea polyphenols. Korean J Soc Obs Gyn 45(4):560-568
  8. Kang HH. 2009. Determination of biological activities of Korean berries and their anthocyanin identification. Ph.D Thesis. Gyeongsang National University. Korea
  9. Chan MY. 2002. Antimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin. Biochem Pharmacol 63(2):99-104 https://doi.org/10.1016/S0006-2952(01)00886-3
  10. Cheon KB. 1999. Screening of antioxidant from Vitis coignetae, Vitis vinifera L. and comparition of its antioxidant activity. M.A Thesis. Konkuk University. Korea
  11. Choi SY, Cho HS, Sung NJ. 2006. The antioxidative and nitrite scavenging ability of solvent extracts from wild grape (Vitis coignetiea) skin. Korean J Soc Food Sci Nutr 35(8): 61-966 https://doi.org/10.3746/jkfn.2006.35.8.961
  12. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res 47:936-942
  13. Chung HY, Park DK. 2003. Antimicrobial activity of grape seed ethanol extract. Korean J Soc Food Sci Nutr 32(1):109-114 https://doi.org/10.3746/jkfn.2003.32.1.109
  14. Conforti F, Statti GA, Menichini F. 2007. Chemical and biological variability of hot pepper fruits (Capsicum annuum var. acuminatum L.) in relation to maturity stage. Food Chem 102:1096-1104 https://doi.org/10.1016/j.foodchem.2006.06.047
  15. Doll R, Peto R. 1981. The causes of cancer: quantitative estimates of avoidable risks of cancer in the United States today. J Natl Cancer Inst 66(6):1191-1308
  16. Filipa V, Plockovaa, M, Smidrkala, J, Spickovaa, Z, Melzochb, K, Schmidtc S. 2003. Resveratrol and its antioxidant and antimicrobial effectiveness. Food Chem 83(4):585-593 https://doi.org/10.1016/S0308-8146(03)00157-2
  17. Ham YA, Choi HJ, Kim SH, Chung MJ, Ham SS. 2009. Antimutagenic and antitumor effect of Adenophora triphylla extracts. J Korean Soc Food Sci Nut 38(1):25-31 https://doi.org/10.3746/jkfn.2009.38.1.025
  18. Huang KS, Lin M, Yu LN, Kong M. 2000. Four novel oligostilbenes from the roots of Vitis amurensis. Tetrahedron 56:1321-1329 https://doi.org/10.1016/S0040-4020(99)01034-0
  19. Hwang JH. 2002. Antioxidative activities of anthocyanins from red leaf mustard (Brassica juncea Coss. var. integrifolia). Ph.D Thesis. Pusan National University. Korea
  20. Kim NY, Choi JH, Kim YG, Jang MY, Moon JH, Park GH, OH DH. 2006. Isolation and identification of an antioxidant substance from ethanol extract of wild grape (Vitis coignetiea) seed). Korean J Food Sci Technol 38(1):109-113
  21. Kim NY, Kim YK, Bae KJ, Choi JH, Moon JH, Park GH, Oh DH. 2005. Free radical scavenging effect and extraction condition of ethanol extracts and fractions of wild grape weed (Vitis coignetiea). Korean J Soc Food Sci Nutr 34(6):755-758 https://doi.org/10.3746/jkfn.2005.34.6.755
  22. Kim SA, Kim J, Woo MK, Kwak CS, MS Lee. 2005. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweed. J Korean Soc Food Sci Nut 34(4):451-459 https://doi.org/10.3746/jkfn.2005.34.4.451
  23. Kim SH. 2008. Optimal condition for deacidification fermentation of wild grape wine by mixed culture. Korean J Soc Appl Biol Chem 51(1):17-23 https://doi.org/10.3839/jabc.2008.004
  24. Kim YH. 2009. Optimum fermentation condition and functional quality characteristics of Meoru wine. Ph.D Thesis. Gyeongsang National University. Korea
  25. Kim YH, Kim DS, Woo JS, Kim HH, Lee YS, Kim HS, Ko KH, Lee SK. 2008. Antioxidant activity and cytotoxicity on human cancer cells of anthocyanin extracted from black soybean. Korean J Crop Sci 54(4):407-412
  26. Ko HS. 1998. Characteristics, and antimutagenic and in vitro anticancer effects of chungkookjang manufactured by various variety of soybean. M.A Thesis. Konkuk University. Korea
  27. Lee DH, Yu HE, Lee JS. 2004. Quality characteristics and physiological functionality of wild grape wine. Korean J Natural Sci 15(1):69-78
  28. Lee DW. 2003. Antioxidant and antimicrobial effects of citrus flavonoids and limonoids. Ph. D. Thesis. Yonsei University. Korea
  29. Lee HJ. 2009. Isolation and identification of anthocyanin in edible rose and determination of their biological activity. M.A. Thesis. Kangwon National University. Korea
  30. Lee HS, Son JY. 2002. Antioxidant and synergist effect of extract isolated from commercial green, oolong and black tea. Korean J Food Nutr 15(4):377-381
  31. Lee MC, Kim KP, Kim SH, Choung NH, Yim MH. 1997. Antimicrobial activity of extract from gall-nut and red-grape husk. Korean J Food Nutr 10(2):174-179
  32. Ludvisson J. 1993. Intervention at diagnosis of type I diabetes using either antioxidants or photopheresis. Diabetes Metab Rev 9(4):329-336 https://doi.org/10.1002/dmr.5610090414
  33. MeCord JM. 2000. The evolution of free radicals and oxidative stress. Am J Med 108:652-662 https://doi.org/10.1016/S0002-9343(00)00412-5
  34. Moreno MI, Isla MI, Sampietro AR, Vattuone MA. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J Ethnopharmacol 71:109-114 https://doi.org/10.1016/S0378-8741(99)00189-0
  35. Nilgun GB, Gulcan O, Osman S. 2004. Total phenolic contents and antibacterial activity of grape extract. Food Control 15:335-339 https://doi.org/10.1016/S0956-7135(03)00083-5
  36. Oh SH, Chung JH, Kim YH, Cho JH. 2006a. Study on antioxidative effects of polyphenols extracted from chestnut inner shell. Korean J Soc Medi Cor Sci 14(10):536-537
  37. Oh SH, Chung JH, Kim YH, Cho JH. 2006b. Study on antioxidative effects of polyphenols extracted from hop. Korean J Soc Cro Sci 51(1):534-535
  38. Park CS. 2005. Antioxidative and nitrite scavenging abilities of medicinal plant extracts. Korean J Food Preserv 12(6):631-636
  39. Park HS. 2010. Physicochemical property and antioxidant activity of wild grape (Vitis coignetiea) juice. Korean J Culinary Res 16(4):297-304
  40. Park HS. 2011. Antioxidant activity of solvent extracts from Vitis coignetiea skins. Korean J Culinary Res 17(1):208-217
  41. Park SJ. Lee HY, Oh DH. 2003 Free radical scavenging of seed and skin extracts from Campbell early grape (Vitis labruscana B.). J Korean Soc Food Sci Nutr 32:115-118 https://doi.org/10.3746/jkfn.2003.32.1.115
  42. Seo DH, Han DS. 2003. Anticancer effects of hexane soluble extract of Houttuynia cordata against cancer cell lines. J Wonkwang dental Research institute 12(1):263-285
  43. Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Visteca D, Warren JT, Bokesch H, Kenney S, Boyd MR. 1988. New colorimetric cytotoxicity assay for anticancer-drug screening. J Nat Cancer Inst 82(13):1107-1112
  44. Son RH, Chin HS, Ham AR, Mar WC, Nam KW. 2010. Antimicrobial activity of resveratrol oilgomaers and flavonoids from stems of Vitis coignetiae Pulliat and the seeds of Perilla rutescens (L.) Britton. Yakhak Hoeji. 54(1):22-26
  45. Suh JS. 2010. The study of Vitis coignetiae rice wine characteristics manufactured by traditional method. Ph.D Thesis. Yeungnam University. Korea
  46. Toda M, Okubo S, lkigai H, Shimamura T. 1990. Antibacterial and anti-hemolysin activities of tea catechins and their structural relatives. Nihon Saikingaku Zasshi. 45(2):561-566 https://doi.org/10.3412/jsb.45.561
  47. Won HR. 2007. Antioxidative activity and serum lipid composition of wild grape seed powder in rats fed hypercholesterolemic diet. Korean J Comm Living Sci 18(3):363-368
  48. Youn JH, Sang HY, Jeon SH, Park HS. 2003. Comparison of resveratrol contents between 'Gailiangmeru'(Vitis spp.) and 'Campbell Early' grape. Kor J Hort Sci Technol 21(Suppl. II):74

Cited by

  1. Hair Dyeing Properties Using Extracts of Wild Grape (Vitis coignetiae) vol.14, pp.3, 2016, https://doi.org/10.20402/ajbc.2016.0065
  2. Physiological Activities of Leaf and Twig Extracts from Lindera obtusiloba Blume vol.29, pp.5, 2013, https://doi.org/10.9724/kfcs.2013.29.5.573
  3. Analysis of Antioxidative and Antimutagenic Activities of Ethanol Extracts from Pericarp and Seeds of Wild Grape (Vitis coignetiea) vol.26, pp.2, 2016, https://doi.org/10.17495/easdl.2016.4.26.2.192