Efficacy of Electrolyzed Water and Aqueous Chlorine Dioxide for Reducing Pathogenic Microorganism on Chinese Cabbage

전기분해수 및 이산화염소수 처리에 따른 배추의 미생물 제어 효과

  • Received : 2011.11.22
  • Accepted : 2012.01.20
  • Published : 2012.04.30


This study evaluated the efficacy of strong acidic electrolyzed water (SAcEW), low alkaline electrolyzed water (LAlEW) and aqueous chlorine dioxide (ACD) for reducing pathogenic bacteria($Escherichia$ $coli$, $Bacillus$ $cereus$, $Salmonella$ Typhimurium, $Stapylococcus$ $aureus$) on Chinese cabbage. Artificially inoculated Chinese cabbage was immersed for 1, 5 and 10 min with TW, NaClO, EW and ACD. Generally, leaves showed more effective reduction than stems. Regarding the inhibitory effect, ACD treatment showed the highest effects rather than other treatments. When Chinese cabbage was immersed for 3 min in sterilized water, it was reduced to a minimum of 1.33 log CFU/g at LAlEW and a maximum of 4.70 log CFU/g at ACD. Compared to NaClO, ACD and LAlEW which showed a reduction of 3.2 log CFU/g ($Sal.$ Typhimurium) and 2.7 log CFU/g($B.$ $cereus$), respectively. Furthermore, the others had similar inhibitory effects compared to NaClO.


electrolyzed water;aqueous chlorine dioxide;pathogenic bacteria;Chinese cabbage;sterilization effect


  1. Bae YM, Hong YJ, Kang DH, Heu SG, Lee SY. Microbial and pathogenic contamination of ready-to-eat fresh vegetables in Korea. Korean J. Food Sci. Technol. 43: 161-168 (2011)
  2. Jo MJ, Jeong AR, Kim HJ, Lee NR, Oh SW, Kim YJ, Chun HS, Koo MS. Microbiogical quality of fresh-cut produce and organic vegetables. Korean J. Food Sci. Technol. 43: 91-97 (2011)
  3. Walls I, Scatt VN. Use of predictive microbiology in microbial food safety risk assessment. Int. J. Food. Microbiol. 36: 97-102 (1997)
  4. Koseki S, Yosida K, Kamitani Y, Itoh K. Efficacy of acidic electrolyzed water for microbial decontamination of cucumbers and strawberries. J. Food Protect. 67: 1247-1251 (2004)
  5. Kim C, Hung YC, Brackett RE, Lin CS. Efficacy of electrolyzed oxidizing water in inactivating Salmonella on alfalfa seeds and sprouts. J. Food Protect. 66: 208-214 (2003)
  6. Ryu SH. Effects of aqueous chlorine dioxide against Escherichia coli O157:H7 and Listeria monocytogenes on broccoli served in foodservice institutions. J. Korean Soc. Food Sci. Nutr. 36: 1622- 1627 (2007)
  7. Kim YJ, Choi KD, Shin IS. Bactericidal activity of strongly acidic electrolyzed water on various vegetables and kitchen apparatus. J. Korean Soc. Food Sci. Nutr. 39: 776-781 (2010)
  8. European Committee for Standardization. Chemical disinfectants and antiseptics (Phase2, step1). European committee for standardization. EN 1276. British Standards Institution. Winterthur, Switzaland (1997)
  9. Russell AD, Hammond SA, Morgan JR. Bacterial resistance to antiseptics and disinfectants. J. Hosp. Infect. 7: 213-224 (1986)
  10. Junli H, Li W, Namqi R, Fang M, Juli. Disinfection effect of chlorine dioxide on bacteria in water. Wat. Res. 31: 607-613 (1997)
  11. Kwon JY, Kim BS, Kim JH. Effect of washing methods and surface sterilization on quality fresh-cut chicory (Clchorium intybus L. var. foliosum). Korean J. Food Sci. Technol. 38: 28-34 (2006)
  12. Guentzel JL, Lam KL, Callan MA, Emmons SA, Dunham VL. Reduction of bacteria on spinach, lettuce, and surfaces in food service areas using neutral electrolyzed oxidizing water. Food Microbiol. 25: 36-41 (2008)
  13. Koo M. Bacillus cereusAn ambusher of food safety. Bull. Food Technol. 22: 587-600 (2009)
  14. EFSA. Opinion of the scientific panel on biological hazards on Bacillus cereus and other Bacillus spp. In foodstuffs. EFSA J. 175: 1-48 (2005)
  15. KFDA. Food Code.10-3-1-43. Korean Food & Drug Administration, Seoul, Korea (2009)
  16. Lee WJ, Lee CH, Yoo JY, Kim KY, Jang KI. Sterilization efficacy of washing method using based on microbubbles and electrolyzed water on various vegetables. J. Korean Soc. Food Sci. Nutr. 40: 912-917 (2011)
  17. Lang MM, Harris LJ, Beuchat LR. Survival and recovery of Escherichia coli O157:H7, Salmonella, and Listeria monocytogenes on lettuce and parsely as affected by method of innoculation, time between inoculation and analysis and treatment with chlorinated water. J. Food Protect. 67: 1092-1103 (2004)
  18. Singh N, Singh RK, Bhunia AK, Stroshine RL. Effect of inoculation and washing methods on the efficacy sanitizers against Escherichia coli O157:H7 on lettuce. Food Mocrobiol. 19: 183- 193 (2002)
  19. Beuchat LR, Adler BB, Lang MM. Efficacy of chlorine and a peroxyacetic acid sanitizer in killing Listeria monocytogenes on iceberg and romaine lettuce using simulated commercial processing conditions. J. Food Protect. 67: 1238-1242 (2004)
  20. Ku KH, Cho MH, Park WS. Characteristics analysis for the standardization of commercial kimchi. Korean J. Food Sci. Technol. 35: 316-319 (2003)
  21. Choi MR, Oh SW, Lee SY. Efficacy of chemical sanitizer in reducing levels of foodborne pathogens and formation of chemically injured cells on cabbage. J. Korean Soc. Food Sci. Nutr. 37: 1337-1342 (2008)
  22. Lee KH. Effect of ozone treatment for sanitation of Chinese cabbage and salted Chinese cabbage. J. Korean Soc. Food Sci. Nutr. 37: 90-96 (2008)
  23. Yoon HH, Lee SY. Quality characteristics of baechu kimchi salted with recycled wastebrine. Korean J. Soc. Food Cookery Sci. 19: 609-615 (2003)
  24. The guide of sanitary management in school food service, Ministry of Education, Science and Technology, Seoul, Korea. p.39 (2010)
  25. Park KJ, Lim JH, Kim BK, Kim JC, Jeong JW, Jeong SW. Effect of aqueous chlorine dioxide and citric acid on reduction of Salmonella Typhimurium on sprouting radish seeds. Korean J. Food Preserv. 15: 754-759 (2008)
  26. Kim MH, Jeong JW, Cho YJ. Comparison of characteristics on electrolyzed water manufactured by various electrolytic factors. Korean J. Food Sci. Technol. 36: 416-422 (2004)
  27. Koseki S, Yoshida K, Isobe S, Itoh K. Decontamination of lettuce using acidic electrolyzed water. J. Food Protect. 64: 652-658 (2001)
  28. Park CM, Hung YC, Doyle MP, Ezeike GOI, Kim C. Pathogen reduction and quality of lettuce treated with electrolyzed oxidizing and acidified chlorinated water. J. Food Sci. 66: 1368-1372 (2001)
  29. Bari ML, Sabina Y, Isobe S, Uemera T, Isshiki K. Effectiveness of electrolyzed acidic water in killing Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes on the surface of tomatoes. J. Food Protect. 66: 542-548 (2003)

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