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Evaluation of the Photoprotective Effect of Dongchongxiacao ($Paecilomyces$ $japonica$) Extract against Ultraviolet Radiation-induced Skin Wrinkling and Cancer

자외선 유도 피부주름 및 암발생에 대한 누에동충하초($Paecilomyces$ $japonica$)의 보호효과 평가

  • Lee, Hae-June (Korea Institute of Radiological & Medical Science) ;
  • Moon, Chang-Jong (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Jong-Choon (College of Veterinary Medicine, Chonnam National University) ;
  • Jung, U-Hee (Advanced Radiation Technology Institute) ;
  • Jo, Sung-Kee (Advanced Radiation Technology Institute) ;
  • Jang, Jong-Sik (Department of Animal Science, Kyungpook National University) ;
  • Kim, Sung-Ho (College of Veterinary Medicine, Chonnam National University)
  • Received : 2012.02.09
  • Accepted : 2012.03.05
  • Published : 2012.03.30

Abstract

To evaluate the ability of Dongchongxiacao ($Paecilomyces$ $japonica$) extract (PJE) to protect the skin from photodamage, the gross and microscopic changes in the skin of hairless mice and PJE-treated mice exposed chronically to ultraviolet (UV) were examined. The skin of the UV-irradiated mice showed characteristic signs of photoaging, such as deep wrinkles across the back. PJE-treated mice showed a significantly decreased wrinkling score. By the 22nd week, 88.9% (i.p. with saline) or 44.4% (topical administration with cream base) of the UV-irradiated mice developed at least one tumor. PJE delayed tumor onset significantly. PJE (i.p.) was also effective in reducing the occurrence of UV radiation-induced skin tumors and reduced the number of tumors per mouse. After 22 weeks of treatment, 80.0% (i.p.) and 75.0% (topical) of the mice treated with PJE were tumor-free. Tumor multiplicity was reduced by 96.2% (i.p.) in the PJE treated groups. It is noted that skin that is chronically exposed to UV is subject to photoaging and photocarcinogenesis and regular use of PJE would prevent these photodamaging effects of UV.

References

  1. Afaq F, Mukhtar H. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Exp. Dermatol. 15(9):678-684; 2006. https://doi.org/10.1111/j.1600-0625.2006.00466.x
  2. Afaq F, Adhami VM, Mukhtar H. Photochemoprevention of ultraviolet B signaling and photocarcinogenesis. Mutat. Res. 571(1-2): 153-173; 2005. https://doi.org/10.1016/j.mrfmmm.2004.07.019
  3. Halliday GM, Lyons JG. Inflammatory doses of UV may not be necessary for skin carcinogenesis. Photochem. Photobiol. 84(2):272-283; 2008. https://doi.org/10.1111/j.1751-1097.2007.00247.x
  4. Bickers DR, Athar M. Oxidative stress in the pathogenesis of skin disease. J. Invest. Dermatol. 126(12):2565-2575; 2006. https://doi.org/10.1038/sj.jid.5700340
  5. Timares L, Katiyar SK, Elmets CA. DNA damage, apoptosis and langerhans cells-Activators of UV-induced immune tolerance. Photochem. Photobiol. 84(2):422-36; 2008. https://doi.org/10.1111/j.1751-1097.2007.00284.x
  6. Matsumura Y, Ananthaswamy HN. Toxic effects of ultraviolet radiation on the skin. Toxicol. Appl. Pharmacol. 195:298-308; 2004. https://doi.org/10.1016/j.taap.2003.08.019
  7. Jemal A, Siegel R, Xu J, Ward E. Cancer statistics. 2010. CA Cancer J. Clin. 60(5):277-300; 2010. https://doi.org/10.3322/caac.20073
  8. Bao ZD, Wu ZG, Zheng F. Amelioration of aminoglycoside nephrotoxicity by Cordyceps sinensis in old patient. Chin. J. Integr. Med. 14:271-273; 1994.
  9. Zhu JS, Halpern GM, Jones K. The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis. Part I. J. Altern. Complement. Med. 4:289-303; 1998. https://doi.org/10.1089/acm.1998.4.3-289
  10. Zhu JS, Halpern GM, Jones K. The scientific rediscovery of an ancient Chinese herbal medicine: Cordyceps sinensis. Part II. J. Altern. Complement. Med. 4:429-457; 1998. https://doi.org/10.1089/acm.1998.4.429
  11. 한대석, 송효남, 김상희. 동충하초 : 새로운 기능성식품 소재. 식품과학과 산업 32:56-63; 1999.
  12. Manabe N, Sugimoto M, Azuma Y, Taketomo N, Yamashita A, Tsuboi H, Tsunoo A, Kinjo N, Nian-Lai H, Miyamoto H. Effects of the mycelial extract of cultured Cordyceps sinensis on in vivo hepatic energy metabolism in the mouse. Jpn. J. Pharmacol. 70:85-88; 1996. https://doi.org/10.1254/jjp.70.85
  13. Won SY, Park EH. Anti-inflammatory and related pharmacological activities of cultured mycelia and-fruiting bodies of Cordyceps militaris. J. Ethnopharmacol. 96:555-561; 2005. https://doi.org/10.1016/j.jep.2004.10.009
  14. Kuo YC, Tsai WJ, Shiao MS, Chen CF, Lin CY. Cordyceps sinensis as an immunomodulatory agent. Am. J. Chin. Med. 24:111-125; 1996. https://doi.org/10.1142/S0192415X96000165
  15. Kuo YC, Lin CY, Tsai WJ, Wu CL, Chen CF, Shiao MS. Growth inhibitors against tumor cells in Cordyceps sinensis other than cordycepin and polysaccharides. Cancer Invest. 12:611-615; 1994. https://doi.org/10.3109/07357909409023046
  16. Li SP, Su ZR, Dong TT, Tsim KW. The fruiting body and its caterpillar host of Cordyceps sinensis show close resemblance in main constituents and anti-oxidation activity. Phytomedicine 9:319-324; 2002. https://doi.org/10.1078/0944-7113-00134
  17. Kiho T, Yamane A, Hui J, Usui S, Ukai S. Polysaccharides in fungi. XXXVI. Hypoglycemic activity of a polysaccharide (CS-F30) from the cultural mycelium of Cordyceps sinensis and its effect on glucose metabolism in mouse liver. Biol. Pharm. Bull. 19:294-296; 1996. https://doi.org/10.1248/bpb.19.294
  18. Kiho T, Hui J, Yamane A, Ukai S. Polysaccharides in fungi. XXXII. Hypoglycemic activity and chemical properties of a polysaccharide from the cultural mycelium of Cordyceps sinensis. Biol. Pharm. Bull. 16:1291-1293; 1993. https://doi.org/10.1248/bpb.16.1291
  19. Shin KH. Screening and evaluation of pharmacologically active principles from cultivated fungus of Cordyceps sp. growing on the silkworm. The Final Repory of the Collaborative Research of National Sericulture and Entomology Research Institute, Rural Development Administration 69-171; 1998.
  20. Shin KH, Lim SS, Lee S, Lee YS, Jung SH, Cho SY. Anti-tumour and immuno-stimulating activities of the fruiting bodies of Paecilomyces japonica, a new type of Cordyceps spp. Phytother. Res. 17:830-833; 2003. https://doi.org/10.1002/ptr.1253
  21. Shin KH, Lim SS, Lee SH, Lee YS, Cho SY. Antioxidant and immunostimulating activities of the fruiting bodies of Paecilomyces japonica, a new type of Cordyceps sp. Ann. N. Y. Acad. Sci. 928:261-273; 2001.
  22. Kim SR, Jo SK, An MY, Shin DH, Kim SH. Antifatique effect and improvement of hematotoxicity by Dongchongxiacao (Paecilomyces japonica). J. Vet. Clin. 19:328-332; 2003.
  23. Kim SR, Oh H, Lee HJ, Shin DH, Kim JC, Park IC, Oh KS, Jo SK, Kim SH. Modification of radiation response in mice by Dongchongxiacao (Paecilomyces japonica). Korean J. Vet. Res. 43:181-188; 2003.
  24. Matsumura Y, Ananthaswamy HN. Toxic effects of ultraviolet radiation on the skin. Toxicol. Appl. Pharmacol. 195:298-308; 2004. https://doi.org/10.1016/j.taap.2003.08.019
  25. Bissett DL, Chatterjee R, Hannon DP. Photoprotective effect of topical anti-inflammatory agents against ultraviolet radiation-induced chronic skin damage in the hairless mouse. Photodermatol. Photoimmunol. Photomed. 7:153-158; 1990.
  26. Ichihashi M, Ueda M, Budiyanto A, Bito T, Oka M, Fukunaga M, Tsuru K, Horikawa T. UV-induced skin damage. Toxicology 189:21-39; 2003. https://doi.org/10.1016/S0300-483X(03)00150-1
  27. Black HS, deGruijl FR, Forbes PD, Cleaver JE, Ananthaswamy HN, deFabo EC, Ullrich SE, Tyrrell RM. Photocarcinogenesis: an overview. J. Photochem. Photobiol. 40(1):29-47; 1997. https://doi.org/10.1016/S1011-1344(97)00021-3
  28. Lee HJ, Kim SR, Kim JS, Kee SA, Kim JC, Bae CS, Jang JS, Jo SK, Kim SH. The effect of Dongchongxiacao (Paecilomyces japonica) on ultraviolet B-induced skin damages in mouse. Lab. Anim. Res. 22(3):203-207; 2006.

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