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감마선 처리에 의한 스프레이형 국화 화색변이체로부터 Flavonoid 3'-Hydroxylase(F3'H) 유전자의 분리 및 특성 구명

Isolation and Characterization of a Novel Flavonoid 3'-Hydroxylase (F3'H) Gene from a Chrysanthemum (Dendranthema grandiflorum) and Its Gamma-ray Irradiated Mutants

  • 정성진 (한국원자력연구원 정읍방사선과학연구소) ;
  • 이긍주 (충남대학교 원예학과) ;
  • 김진백 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김동섭 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김상훈 (한국원자력연구원 정읍방사선과학연구소) ;
  • 강시용 (한국원자력연구원 정읍방사선과학연구소)
  • Chung, Sung-Jin (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University) ;
  • Kim, Jin-Baek (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong-Sub (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang-Hoon (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 투고 : 2011.06.23
  • 심사 : 2011.10.11
  • 발행 : 2012.04.30

초록

스프레이 국화품종 'Argus'와 감마선 조사에 의해 화색변이가 일어난 돌연변이체의 꽃잎으로부터 안토시아닌 생합성 경로에서 중요한 역할을 담당하는 신규 $DgF3'H$의 전장 cDNA와 genomic DNA를 분리하였다. 전장 cDNA는 1,527bp(509 아미노산)의 ORF를 포함하고 있으며, 원품종 'Argus'와 화색변이체 사이의 염기서열 상동성은 97% 이상을 나타내었다. Genomic DNA의 크기는 야생형 'Argus'에서 3,831bp이었고, 3가지 화색 변이체에서는 3,828부터 3,838bp의 크기를 나타내었다. $DgF3'H$ 유전자는 세 개의 exon사이에 두개의 intron을 갖고 있는 구조이고, 3'과 5' UTR 부분을 제외한 intron의 크기는 야생형 'Argus'에서 2,157bp이지만 3가지 화색 변이체에서는 2,155부터 2,159bp의 크기를 갖고 있었다. 이것은 감마선 조사에 의해 intron 부분의 유전자가 결실 또는 삽입된 것으로 추정된다. Southern 분석 결과 국화의 genome 내에서는 복수의 F3'H 유전자를 갖는 것이 확인되었다. $DgF3'H$ 유전자의 발현 정도를 분석한 결과, 연분홍의 'Argus'와 두 개의 보라색 변이체(AM1 and AM3)에서 높게 발현되었으나 흰색 변이체(AM2)에서는 매우 약하게 발현되었으며, 염기서열 변이에 의한 F3'H 유전자의 구조적 차이가 화색의 변이에 관련된 것으로 추정되었다. 국화 'Argus' 및 화색 변이체를 이용하여 본 연구에서 분리한 신규 F3'H 유전자의 구조 및 유전자 발현 등을 포함하는 유전정보들은 화색 변이의 유전적 기작을 밝히는데 중요한 자료로 이용될 것으로 기대되나 향후 다른 유전적 발현요소들이 국화의 F3'H 유전자의 발현에 관여하는지에 관한 추가적인 연구가 필요하다고 하겠다.

과제정보

연구 과제 주관 기관 : 한국연구재단

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  1. Development of a New Coreopsis Variety 'Uridream Pink' by Gamma-ray Irradiation vol.32, pp.6, 2014, https://doi.org/10.7235/hort.2014.14074