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Isolation and Characterization of a Novel Flavonoid 3'-Hydroxylase (F3'H) Gene from a Chrysanthemum (Dendranthema grandiflorum) and Its Gamma-ray Irradiated Mutants

감마선 처리에 의한 스프레이형 국화 화색변이체로부터 Flavonoid 3'-Hydroxylase(F3'H) 유전자의 분리 및 특성 구명

  • Chung, Sung-Jin (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Geung-Joo (Department of Horticulture, Chungnam National University) ;
  • Kim, Jin-Baek (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Dong-Sub (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang-Hoon (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Radiation Breeding and Research Team, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 정성진 (한국원자력연구원 정읍방사선과학연구소) ;
  • 이긍주 (충남대학교 원예학과) ;
  • 김진백 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김동섭 (한국원자력연구원 정읍방사선과학연구소) ;
  • 김상훈 (한국원자력연구원 정읍방사선과학연구소) ;
  • 강시용 (한국원자력연구원 정읍방사선과학연구소)
  • Received : 2011.06.23
  • Accepted : 2011.10.11
  • Published : 2012.04.30

Abstract

The objectives of this study were to isolate and the sequence of novel $F3'H$ gene related to an anthocyanin pathway, and to confirm the expression patterns of the gene involved in the flower color variations of chrysanthemum mutants. In this study, we isolated the full-length cDNAs and the genomic DNAs of an $F3'H$ gene from a wild type (WT) chrysanthemum (cv. Argus) and its three color mutants. The sequence analysis revealed a putative open reading frame of 1,527 bp that encodes a polypeptide of 509 amino acids. Sequence homology ranged from 97% to 99% between 'Argus' and its three color mutants. The sequence analysis from the genomic DNA revealed that the chrysanthemum $DgF3'H$ gene consisted of three exons and two introns spanning a 3,830 bp length. The sizes of the gene for three mutants ranged from a shorter size of 3,828 bp to a longer size of 3,838 bp when compared to the size of WT. The total size of the two introns was 2,157 bp for WT, but those for three color mutants ranged from 2,154 bp to 2,159 bp. A result of an RT-PCR analysis indicated that the color variations of the mutants AM1 and AM2 can be partly explained by the structural modification derived from the sequencial changes in the gene caused by gamma ray. A Southern blot analysis revealed that the $DgF3'H$ gene existing as multiple copies in the chrysanthemum genome. A systemic study will be further needed to provide a genetic mechanism responsible for the color mutation and to uncover any involvement of genetic elements for the expression of the $DgF3'H$ gene for the color variation in chrysanthemum.

Acknowledgement

Supported by : 한국연구재단

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