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Effect of Amides as a Cryoprotectant on Quality of Frozen-thawed Sperm in Korean Jeju Black Bull

제주흑우 동결정액 제조 시 Amide 계열의 동결보호제가 동결 융해 후 정자의 성상에 미치는 영향

  • Oh, Shin-Ae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Choi, Sun-Ho (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Ko, Min-Hee (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Kang, Tae-Young (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Ko, Moon-Suck (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Oh, Young-Mi (Department of Veterinary Medicine, Jeju National University) ;
  • Cho, Won-Mo (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
  • 오신애 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 최선호 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 고민희 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 강태영 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 조상래 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 고문석 (농촌진흥청 국립축산과학원 난지축산시험장) ;
  • 오영미 (국립제주대학교 수의과대학) ;
  • 조원모 (농촌진흥청 국립축산과학원 난지축산시험장)
  • Received : 2012.04.10
  • Accepted : 2012.04.23
  • Published : 2012.04.30

Abstract

The objective of this study was to examine the effect of amides as a cryoprotectant for semen cryopreservation in Korean Jeju Black Bull. The semen was cryopreserved with extenders containing 5% dimethyl acetamide (DMA), 5% dimethyl formamide (DMF), 5% methyl formamide (MF) or 7% glycerol. Post-thawed sperm were evaluated for sperm motility, viability, acrosome integrity and membrane integrity. Post-thawed sperm motility was significantly higher (p<0.05) in glycerol and DMF ($64.00%{\pm}9.62$ and $59.00%{\pm}5.48$, respectively) than DMA and MF ($50.00%{\pm}3.24$ and $44.00%{\pm}4.18$, respectively). Sperm viability wassignificantly higher (p<0.05) in glycerol and DMF ($58.25%{\pm}7.35$ and $53.05%{\pm}3.77$, respectively) than others. However, for sperm motility and viability, there were no differences among glycerol and DMF. Also, swelling sperm ratio by hypo-osmetic selling test (HOST) was significantly increased (p<0.05) in glycerol and DMF treatments ($45.12%{\pm}25.08$ and $44.95%{\pm}8.58$, respectively). The percentage of capacitated sperm assessed by CTC staining, F pattern was lower (p<0.05) in DMF than others. B pattern was increased (p<0.05) in DMA, DMF and MF when compared with glycerol. AR pattern ratio was decreased (p<0.05) in glycerol and DMF when compared with DMA and MF. These results suggested that amides performed better and could be used as a cryoprotectant for semen freezing of Korean Jeju Black Bull.

Keywords

Korean Jeju Black Bull;Semen freezing;Cryoprotectants;Amide compound

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