The Estrus Manifestation according to the Follicle Size and the Growth Phase after $PGF_{2{\alpha}}$ Injection in Korean Native Cows

한우에서 $PGF_{2{\alpha}}$ 투여 후 난포 크기 및 발달 과정에 따른 발정 증상의 발현

  • Park, Chul-Ho (College of Veterinary Medicine, Chonnam National University) ;
  • Ryu, Jae-Sun (College of Veterinary Medicine, Chonnam National University) ;
  • Yu, Dae-Jung (Livestock Research Institute, JARES) ;
  • Park, In-Chul (School of Veterinary Medicine, Kangwon National University) ;
  • Kim, Jong-Taek (School of Veterinary Medicine, Kangwon National University) ;
  • Suh, Guk-Hyun (College of Veterinary Medicine, Chonnam National University) ;
  • Oh, Ki-Seok (College of Veterinary Medicine, Chonnam National University) ;
  • Son, Chang-Ho (College of Veterinary Medicine, Chonnam National University)
  • 박철호 (전남대학교 수의과대학) ;
  • 류재선 (전남대학교 수의과대학) ;
  • 유대중 (전라남도 농업기술원 축산연구소) ;
  • 박인철 (강원대학교 수의(학부)대학) ;
  • 김종택 (강원대학교 수의(학부)대학) ;
  • 서국현 (전남대학교 수의과대학) ;
  • 오기석 (전남대학교 수의과대학) ;
  • 손창호 (전남대학교 수의과대학)
  • Received : 2012.08.16
  • Accepted : 2012.08.30
  • Published : 2012.09.30

Abstract

This study was operated to establish induction using ultrasonography by estimating the relation of follicle size and estrus manifestation. Clinical estrus symptoms were observed 97.4% in cows and 87.5% in heifers when overall 55 cows were induced to estrus in a single dose of $PGF_{2{\alpha}}$ after verifying CL through ultrasonography, which means estrus hours among those 52 cows showing the clinical estrus symptoms were estimated 2.39 days on cows and for 2.37 days on heifers which showed no differences (p>0.05). The estrus manifestation hours according to the follicle size in cows didn't have any significance each other (p>0.05), though estrus hours was 54 hours (the shortest) with follicle size bigger than 10 mm and were made up within 69 hours. The estrus manifestation hours according to the follicle size in heifers didn't have any significance each other (p>0.05) and took around 42 hours (the shortest) with follicle size of 5mm (the smallest) and were made up within 66 hours. Follicles after $PGF_{2{\alpha}}$ injection were ovulated and assigned to many phases as follows; Group 1 (growing phase) - continuously growing into ovulation, Group 2 (growing and static phase) - delaying in growth after the growth of follicles, Group 3 (static and growing phase) - growing after growth delay, Group 4 (regressing and new growing phase) - the follicle is closed and a new follicle grows. In addition, the process of follicle development and estrus hours had no significance each other (p>0.05), though estrus manifestation hours in Group 1 and 2 was relatively short, and in Group 3 and 4 for a relatively long time. In the result of all above, the estrus manifestation hours after $PGF_{2{\alpha}}$ injection has no differences accoring to the follicle size in cows and heifers. Therefore, High pregnancy rate is obtained when practicing artificial insemination within 3 days in estrus or TAI in 72 to 80 hours after adminitrating $PGF_{2{\alpha}}$.

References

  1. Diskin MG, Austin EJ and Roche JF. 2002. Exogenous hormonal manipulation of varian activity in cattle. Domes. Anim. Endoc. 23:211-228. https://doi.org/10.1016/S0739-7240(02)00158-3
  2. Eddy RG. 1977. Cloprostenol as a treatment for no visible oestrus and cystic ovarian disease in dairy cows. Vet. Rec. 100:62-65. https://doi.org/10.1136/vr.100.4.62
  3. Edmondson AJ, Fissore RA, Rashen RL and Bondurant RH. 1986. The use of ultrasonography for the pathological ovarian structure. Anim. Reprod. Sci. 2:157-165.
  4. Edmonson AJ, Lean IJ, Weaver LD, Farver T and Webster G. 1989. A body condition scoring chart for Holstein dairy cows. J. Dairy Sci. 72:68-78. https://doi.org/10.3168/jds.S0022-0302(89)79081-0
  5. Fortune JE, Sirois J, Turzillo AM and Lavoir M. 1991. Follicle selection in domestic ruminants. J. Reprod. Fertil. Suppl. 43:187-198.
  6. Kastelic JP, Bergfelt DR and Ginther OJ. 1990a. Relationship between ultrasonic assessment of the corpus luteum and plasma progesterone concentration in heifers. Theriogenology 33:1269-1278. https://doi.org/10.1016/0093-691X(90)90045-U
  7. Kastelic JP, Knopf L and Ginther OJ. 1990b. Effect of day of prostaglandin $F_{2\alpha}$ treatment on selection and development of the ovulatory follicle in heifers. Anim. Reprod. Sci. 23: 169-180. https://doi.org/10.1016/0378-4320(90)90001-V
  8. Lopez-Gatius F, Yaniz JL, Santolaria P, Murugavel K, Guijarro R, Calvo R and Lopez-Bejar M. 2004. Reproductive performamce of lactating dairy cows treated with cloprostenol at the time of insemination. Theriogenology 62:677-689. https://doi.org/10.1016/j.theriogenology.2003.11.014
  9. Lucy MC, Savio JD, De Badinga L, La Sota RL and Thatcher WW. 1992. Factors that affect ovarian follicular dynamics in cattle. J. Anim. Sci. 70:3615-3620. https://doi.org/10.2527/1992.70113615x
  10. MacMillan KL and Henderson HV. 1983. Analyses of the variation in the interval from an injection of prostaglandin $F_{2\alpha}$ to oestrus as a method of studying patterns of follicle development during dioestrus in dairy cows. Anim. Reprod. Sci. 6:245-254.
  11. MacMillan KL, Day AM and Smith JF. 1980. Onset of oestrus and fertility in lactating dairy cows injected with an analogue of prostaglandin $F_{2\alpha}$, cloprostenol. Anim. Reprod. Sci. 3:171-180. https://doi.org/10.1016/0378-4320(80)90013-5
  12. Martin SW. 1987. The management of drug-induced manipulation of the estrous cycle in normal cows and heifers. Can. Vet. J. 28:366-373.
  13. Odd KG. 1990. A review of synchronization of estrus in postpartum cattle. J. Anim. Sci. 68:817-830. https://doi.org/10.2527/1990.683817x
  14. Pierson RA and Ginther OJ. 1987. Reliability of diagnostic ultrasonography for identification and measurement of follicles and detecting the corpus luteum in heifers. Theriogenology 28:929-936. https://doi.org/10.1016/0093-691X(87)90043-4
  15. Savio JD, Boland MP, Hynes N, Mattiacci MR and Roche JF. 1990. Will the first dominant follicle of the estrous cycle of heifers ovulate following luteolysis on day 7?. Theriogenology 33:677-687. https://doi.org/10.1016/0093-691X(90)90545-5
  16. Sirois I and Fortune JE. 1988. Ovarian follicular dynamics during the estrous cycle in heifers monitored by real time ultrasonography. Biol. Reprod. 39:308-317. https://doi.org/10.1095/biolreprod39.2.308
  17. Smith ST, Ward WR and Dobson H. 1998. Use of ultrasonography to help to predict observed oestrus in dairy cows after the administration of prostaglandin $F_{2}$ alpha. Vet. Rec. 142:271-274. https://doi.org/10.1136/vr.142.11.271
  18. Stevenson JS, Lamb GC, Cartmill JA, Hensley BA, El-Zarkouny SZ and Marple TJ. 1999. Synchronizing estrus in replacement beef heifers using GnRH, melengestrol acetate, and $PGF_{2\alpha}$. J. Anim. Sci. 77:225.
  19. Stevenson JS, Schmidt MK and Call EP. 1984. Stage of estrous cycle, time of insemination, and seasonal effects on estrus and fertility of Holstein heifers after prostaglandin $F_{2\alpha}$. J. Dairy Sci. 67:1798-1805. https://doi.org/10.3168/jds.S0022-0302(84)81507-6
  20. Tanabe TY and Hann RC. 1984. Synchronized estrus and subsequent conception in dairy heifers treated with prostaglandin $F_{2\alpha}$. Influence of stage of cycle at treatment. J. Anim. Sci. 58:805-811. https://doi.org/10.2527/jas1984.584805x
  21. Turnera TB, Petersona GA, Davisa ME, Wilsona GR, Irvina KM and Forrya JTT. 1987. Synchronization of estrus in beef cows and heifers with fenprostalene, cloprostenol sodium, and prostaglandin $F_{2}$alpha. Theriogenology 28:15-24. https://doi.org/10.1016/0093-691X(87)90181-6
  22. 강병규, 최한선, 손창호, 오기석, 강현구, 김삼주, 김혁진, 김남기. 1995. Progesterone 농도측정에 의한 유우의 번식효율증진에 관한 연구. V. 혈중 progesterone 농도측정에 의한 무발정의 감별진단 및$PGF_{2\alpha}$또는 GnRH 치료효과의 판정. 대한수의학회지 35:603-613.
  23. 손창호, 강병규, 최한선. 1995. 젖소에서 발정주기중 초음파 진단장치로 측정된 황체의 크기와 progesterone 농도와의 관계. 대한수의학회지 35:833-841.