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Preliminary Study of Protective Effects of Flavonoids against Radiation-induced Lung Injury in Mice

  • Wang, Juan (Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences) ;
  • Xu, Heng-Wei (Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences) ;
  • Li, Bao-Sheng (Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences) ;
  • Zhang, Jian (Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences) ;
  • Cheng, Jian (Department of Radiation Oncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences)
  • Published : 2012.12.31

Abstract

Background: Radiation therapy plays an important role in lung carcinoma treatment. However, the incidence of symptomatic radiation-induced lung injury is high. This study aimed to evaluate radioprotective effects of flavonoids extracted from Astragalus complanatus and mechanisms of action against radiation damage. Methods: Alteration in antioxidant status and levles of several cytokines were investigated in BABL/C mice treated with 4 mg/kg b.wt. flavonoids after exposure to 10Gy thoracic radiation. Results: Serum levels of SOD in the flavonoids+radiation group were significantly higher compared to the radiation control group, while TGF-${\beta}1$ and IL-6 were lower. Mice in the radiation control group displayed more severe lung damage compared with the flavonoids+radiation group. The expression of TGF-${\beta}1$ and TNF-${\alpha}$ in the radiation control group was markedly increased in alveolar epithelial cells and macrophages of the alveolar septum. Conclusions: From the results of the present study, flavonoids could be excellent candidates as protective agents against radiation-induced lung injury.

Keywords

Radiation-induced lung injury;flavonoids of Astragalus complanatus;ELISA;immunohistochemistry

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