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Saliva Supernatant miR-21: a Novel Potential Biomarker for Esophageal Cancer Detection

  • Xie, Zi-Jun (Department of Gastroenterology, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Chen, Gang (Department of Thoracic Surgery, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Zhang, Xu-Chao (Guangdong Lung Cancer Institute, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Li, Dong-Feng (Research Center of Medical Sciences, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Huang, Jian (Physical Examination Center of East Ward, Guangdong Academy of Medical Sciences, Guangdong General Hospital) ;
  • Li, Zi-Jun (Department of Gastroenterology, Guangdong Academy of Medical Sciences, Guangdong General Hospital)
  • Published : 2012.12.31

Abstract

Objective: To identify whether saliva supernatant miR-21 can serve as a novel potential biomarker in patients with esophageal cancer (EC). Methods: 32 patients with EC and 16 healthy controls were recruited in this study. Total RNA was extracted from saliva supernatant samples for measurement of miR-21 levels using RT-qPCR and relationships between miR-21 levels and clinical characteristics of EC patients were analyzed. Results: miR-21 was significantly higher in the EC than control groups. The sensitivity and specificity were 84.4% and 62.5% respectively. Supernatant miR-21 levels showed no significant correlation with cancer stage, differentiation and nodal metastasis. Conclusions: Saliva supernatant miR-21 may be a novel biomarker for EC.

Keywords

Esophageal cancer;microRNA;diagnosis;biomarker

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