Human Papilloma Virus 18 Detection in Oral Squamous Cell Carcinoma and Potentially Malignant Lesions Using Saliva Samples

  • Goot-Heah, Khor (Centre of Studies for Preclinical Science, Faculty of Dentistry, Universiti Teknologi MARA) ;
  • Kwai-Lin, Thong (Institute of Biological Science, Faculty of Science, University of Malaya) ;
  • Froemming, Gabriele Ruth Anisah (Institute for Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA) ;
  • Abraham, Mannil Thomas (Tengku Ampuan Rahimah Hospital, Department of Oral and Maxillofacial Surgery, Ministry of Health Malaysia) ;
  • Rosdy, Nik Mohd Mazuan Nik Mohd (Centre of Studies for Oral and Maxillofacial Diagnostic and Medicine, Faculty of Dentistry, Universiti Teknologi MARA) ;
  • Zain, Rosnah Binti (Oral Cancer Research and Coordinating Centre (UM-OCRCC), Faculty of Dentistry, University of Malaya)
  • Published : 2012.12.31


Background: Oral cancer has become one of the most prevalent cancers worldwide and human Papillomavirus is one of the risk factors for developing oral cancer. For this study HPV18 was chosen as it is one of the high risk HPV types and may lead to carcinogenesis. However, prevalence of HPV18 infection in Oral Squamous Cell Carcinoma in Malaysia remains unclear. Objective: This study aimed to investigate the viral load of HPV18 DNA in OSCC and potentially malignant lesions using saliva samples. Materials and Methods: Genomic DNAs of thirty saliva samples of normal subjects and thirty saliva samples compromised of 16 samples from potentially malignant lesions and 14 of OSCC patients were amplified for HPV18 DNA using a nested polymerase chain reaction analysis. All PCR products were then analyzed using the Bioanalyzer to confirm presence of HPV18 DNA. Result: From thirty patients examined, only one of 30 (3.3%) cases was found to be positive for HPV18 in this study. Conclusion: The finding of this study revealed that there is a low viral detection of HPV18 in Malaysian OSCC by using saliva samples, suggesting that prevalence of HPV18 may not be important in this group of Malaysian OSCC.


HPV 18;oral squamous cell carcinoma;nested PCR;Malaysia


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