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Screening for in vitro Cytotoxic Activity of Seaweed, Sargassum sp. Against Hep-2 and MCF-7 Cancer Cell Lines

  • Mary, J. Stella (Post Graduate Department of Immunology and Microbiology, The American College) ;
  • Vinotha, P. (Post Graduate Department of Immunology and Microbiology, The American College) ;
  • Pradeep, Andrew M. (Post Graduate Department of Immunology and Microbiology, The American College)
  • Published : 2012.12.31

Abstract

Discovery of anticancer drugs that kill or disable tumor cells in the presence of normal cells without undue toxicity is a potential challenge for therapeutic care. Several papers in the literature have emphasized the potential implications of marine products such as seaweeds which exhibit antitumor activity. Study attempts to screen the antitumor effect of Sargassum sp, against chosen cell lines such as MCF-7 (Breast cancer) and Hep-2 (Liver Cancer). Ethanol extract of Sargassum sp. was concentrated using a Soxhlet apparatus and dissolved in DMSO. In vitro cytotoxic activity of Sargassum sp at various concentrations ($100{\mu}g/ml-300{\mu}g/ml$) screened for antitumor effect against the chosen cell lines using MTT assay (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, a yellow tetrazole). The study documented that the percentage of cell viability has been reduced with increased concentration, as evidenced by cell death. Sargassum sp extract shows potential cytotoxic activity ($P{\leq}0.05$) with $IC_{50}$ of $200{\mu}g/ml$ and $250{\mu}g/ml$ against Hep-2 and MCF-7 cell lines respectively. The ethanol fraction of Sargassum sp induced cell shrinkage, cell membrane blebbing and formation of apoptotic bodies with evidence of bioactive components as profound influencing factors for anti-tumor effects. Further research need to be explored for the successful application of Sargassum sp as a potent therapeutic tool against cancer.

Keywords

MTT;Sargassum sp.;apoptosis;HEP 2;MCF 7

References

  1. Zandi K, Tajbakhsh S, Nabipour I, et al (2010). In vitro antitumor activity of Gracilaria corticata (a red alga) against jurkat and molt-4 human cancer cell lines. African J Biotechnology, 9, 6787-90.
  2. Berlinck RG, Ogawa CA, Almeida AM, et al (1996). Chemical and pharmacological characterization of halitoxin from Amphimedon viridis (Porifera) from the southeastern Brazilian coast. Comp Biochem Physiol Clin Pharmacol Toxicol Endocrinol, 115, 155-63. https://doi.org/10.1016/S0742-8413(96)00107-7
  3. Blunden G (1993). Marine algae as sources of biologically active compounds. Interdiscip Sci Rev, 18, 73-80. https://doi.org/10.1179/030801893789767158
  4. Booth E (1964). Trace elements and seaweeds. In: De Virville, A.D., Feldmann, J. (Eds.), Proceeding of the 4th International seaweed symposium. Macmillan, London, pp. 385-393.
  5. Carte BK (1996). Biomedical potential of marine natural products. Bioscience, 46, 271-86. https://doi.org/10.2307/1312834
  6. Dharallear VK, Kavleekas D (2004). Seaweed - a field manual national insititute of oceanography Dunapoula Goa.
  7. Gerwick WH, Bernart MW (1993). In: Marine Biotechnology. (Eds.): D. Attaway and O. Zaborsky. Plenum Press, New York. pp. 101-152.
  8. Hameed S, Sultana V, Ara J, Ehteshamul-Haque S, Athar M (2009). Toxicity of fusarium solani strains on brine shrimp (Artemia salina). Zoological Res, 30, 468-72. https://doi.org/10.3724/SP.J.1141.2009.04468
  9. Harada H, Noro T, Kamei Y (1997). Selective antitumor activity in vitro from marine algae from Japan coast. Biol Pharm Bull, 20, 541-6. https://doi.org/10.1248/bpb.20.541
  10. Hu X, Jiang X, Hwang H, Liu S, Guan H (2004). Antitumour activities of alginate-derived oligosaccharides and their sulphated substitution derivatives. Eur J Phycol, 39, 67-71. https://doi.org/10.1080/09670260310001636695
  11. Ly BM, Buu NQ, Nhut ND, et al (2005). Studies on fucoidan and its production from Vietnamese brown sea weeds. AJSTD, 22, 371-80.
  12. Manilal A, Sujith S, Kiran GS, Selvin J, Shikar C (2009). Cytotoxic potentials of red alga, laurencia brandenii collected from the Indian Coast. Global J Pharmacol, 3, 90-4.
  13. Sambarmurty AVSS (2005). A text book of algae I.K International pvt Ltd, New delhi, 97-65.
  14. Smit AJ (2004). Medicinal and pharmaceutical uses of seaweed natural products: a review. J Appl Phycol, 16, 245-62. https://doi.org/10.1023/B:JAPH.0000047783.36600.ef
  15. Song L, Ren S, Yu R, et al (2008). Purification, characterization and in vitro anti-tumor activity of proteins from arca subcrenata lischke. Marine Drugs, 6, 418-30. https://doi.org/10.3390/md6030418
  16. Synytsya A, Kim WJ, Kim SM, et al (2010). Structure and antitumor activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida. Carbohydrate Polymers, 81, 41-8. https://doi.org/10.1016/j.carbpol.2010.01.052
  17. Tang HF, Yang-Hua Y, Yao XS, et al (2002). Bioactive steroids from the brown alga Sargassum carpophyllum. J Asian Nat Prod Res, 4, 95-105. https://doi.org/10.1080/10286020290027362
  18. Xu N, Fan X, Yan X, Tseng C (2004). Screening marine algae from China for their antitumor activities. J Appl Phycol, 16, 451-6. https://doi.org/10.1007/s10811-004-5508-x
  19. Xu H, Yao L, Sung H, Wu L (2009). Chemical composition and antitumor activity of different polysaccharides from the roots Actinidia eriantha. Carbohydr Pol, 78, 316-22. https://doi.org/10.1016/j.carbpol.2009.04.007
  20. Albano RM, Pavao MS, Mourao PA, Mulloy B (1990). Structural studies of a sulfated L-galactan from Styela plicata (Tunicate): analysis of the smith-degraded polysaccharide. Carbohydr. Res, 208, 163-74. https://doi.org/10.1016/0008-6215(90)80096-L
  21. Ayesha, Hira, Sultana V, Ara J, Ehteshamul-Haque S (2010). In vitro cytotoxicity of seaweeds from Karachi coast on brine shrimp. Pak J Bot, 42, 3555-60.

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