Resveratrol Affects Protein Kinase C Activity and Promotes Apoptosis in Human Colon Carcinoma Cells

  • Fang, Jie-Yu (Department of Anesthesia, Sun Yat-sen University) ;
  • Li, Zhi-Hua (Department of Gynecology&Obstetrics, The Third Affiliated Hospital, Guangzhou Medical College) ;
  • Li, Qiang (Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University) ;
  • Huang, Wen-Sheng (Department of Surgery, The First Affiliated Hospital, Sun Yat-sen University) ;
  • Kang, Liang (Department of Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University) ;
  • Wang, Jian-Ping (Department of Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University)
  • Published : 2012.12.31


Background: Resveratrol has been reported to have potential chemopreventive and apoptosis-inducing properties in a variety of tumor cell lines. Objective: In this study, to investigate the effects of resveratrol on protein kinase C (PKC) activity and apoptosis in human colon carcinoma cells, we used HT-29 cells and examined the $PKC{\alpha}$ and ERK1/2 signaling pathways. Methods: To test the effects of resveratrol on the growth of HT-29 cells, the cells were exposed to varying concentrations and assessed with the the MTT cell-viability assay. Fluorescence-activated cell sorter (FACS) analysis was applieded to determine the effects of resveratrol on cell apoptosis. Western blotting was performed to determine the protein levels of $PKC{\alpha}$ and ERK1/2. In inhibition experiments, HT-29 cells were treated with G$\ddot{o}$6976 or PD98059 for 30 min, followed by exposure to $200{\mu}M$ resveratrol for 72 h. Results: Resveratrol had a significant inhibitory effect on HT-29 cell growth. FACS revealed that resveratrol induced apoptosis. Western blotting showed that e phosphorylation of $PKC{\alpha}$ and ERK1/2 was significantly increased in response to resveratrol treatment. Pre-treatment with $PKC{\alpha}$ and ERK1/2 inhibitors (G$\ddot{o}$6976 and PD98059) promoted apoptosis. Conclusion: Resveratrol has significant anti-proliferative effects on the colon cancer cell line HT-29. The PKC-ERK1/2 signaling pathway can partially mediate resveratrol-induced apoptosis of HT-29 cells.


Resveratrol;HT;29 cells;apoptosis;PKC;ERK1/2 signaling pathway


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