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Up-Regulation of NAG-1 and p21 Genes by Sulforaphane

브로콜리 유래 sulforaphane에 의한 NAG-1과 p21 유전자의 발현 조절

  • Received : 2011.11.17
  • Accepted : 2012.02.17
  • Published : 2012.03.30

Abstract

We investigated the anti-proliferative activity of sulforaphane and expression changes of NAG-1 and p21 genes in response to sulforaphane treatment in human colorectal HCT116 cells. The results showed that sulforaphane decreased cell viabilities in a dose-dependent manner and induced expression of NAG-1 and p21 proteins in a dose-dependent and time-dependent manner. In addition, we found that NAG-1 expression by sulforaphane was not dependent on the presence of p53, whereas p21 expression was dependent on p53 presence. The results indicated that up-regulation of NAG-1 was not related with the activity of a dietary histone deacetylase inhibitor of sulforaphane. ATF3 induction was detected from 2 hr after sulforaphane treatment, indicating that ATF3 could be a transcription factor to up-regulate NAG-1 expression. The results of this study may help to increase our understanding of the molecular mechanism of anti-cancer activity mediated by sulforaphane in human colorectal cancer cells.

Keywords

Sulforaphane;anti-proliferative activity;NAG-1;p21;colorectal cancer cell

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