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Phloroglucinol Inhibits the in vitro Differentiation Potential of CD34 Positive Cells into Endothelial Progenitor Cells

  • Kwon, Yi-Hong (Department of Biomedical Science, Laboratory for Functional Foods & Nutrigenomics, Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Jun-Hee (Laboratory for Vascular Medicine & Stem Cell Biology, Research Institute of Convergence of Biomedical Science and Technology, Department of Physiology, Pusan National University Yangsan Hospital) ;
  • Jung, Seok-Yun (Laboratory for Vascular Medicine & Stem Cell Biology, Research Institute of Convergence of Biomedical Science and Technology, Department of Physiology, Pusan National University Yangsan Hospital) ;
  • Kim, Jae-Won (Department of Biomedical Science, Laboratory for Functional Foods & Nutrigenomics, Department of Food Science and Biotechnology, CHA University) ;
  • Lee, Sang-Hun (Laboratory for Vascular Medicine & Stem Cell Biology, Research Institute of Convergence of Biomedical Science and Technology, Department of Physiology, Pusan National University Yangsan Hospital) ;
  • Lee, Dong-Hyung (Department of Obstetrics and Gynecology, Pusan National University College of Medicine and Medical Research Institute, Pusan National University) ;
  • Lee, Kyu-Sup (Department of Obstetrics and Gynecology, Pusan National University College of Medicine and Medical Research Institute, Pusan National University) ;
  • Lee, Boo-Yong (Department of Biomedical Science, Laboratory for Functional Foods & Nutrigenomics, Department of Food Science and Biotechnology, CHA University) ;
  • Kwon, Sang-Mo (Laboratory for Vascular Medicine & Stem Cell Biology, Research Institute of Convergence of Biomedical Science and Technology, Department of Physiology, Pusan National University Yangsan Hospital)
  • Received : 2011.10.12
  • Accepted : 2011.11.23
  • Published : 2012.03.31

Abstract

Inhibiting the bioactivities of circulating endothelial progenitor cells (EPCs) results in significant inhibition of neovessel formation during tumor angiogenesis. To investigate the potential effect of phloroglucinol as an EPC inhibitor, we performed several in vitro functional assays using $CD34^+$ cells isolated from human umbilical cord blood (HUCB). Although a high treatment dose of phloroglucinol did not show any cell toxicity, it specifically induced the cell death of EPCs under serum free conditions through apoptosis. In the EPC colony-forming assay (EPC-CFA), we observed a significant decreased in the small EPC-CFUs for the phloroglucinol group, implying that phloroglucinol inhibited the early stage of EPC commitment. In addition, in the in vitro expansion assay using $CD34^+$ cells, treatment with phloroglucinol was shown to inhibit endothelial lineage commitment, as demonstrated by the decrease in endothelial surface markers of EPCs including $CD34^+$, $CD34^+/CD133^+$, $CD34^+/CD31^+$ and $CD34^+/CXCR4^+$. This is the first report to demonstrate that phloroglucinol can inhibit the functional bioactivities of EPCs, indicating that phloroglucinol may be used as an EPC inhibitor in the development of biosafe anti-tumor drugs that target tumor angiogenesis.

Acknowledgement

Supported by : National Research Foundation

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