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Isolation and Characterization of Duck Feather-Degrading Microorganism for Treatment of Recalcitrant Keratinous Waste

난분해성 케라틴 폐기물 처리를 위한 우모 분해 미생물의 분리 및 특성

  • Go, Tae-Hun (Department of Life Science and Environmental Biochemistry, Pusan National University) ;
  • Jeong, Jin-Ha (Department of Life Science and Environmental Biochemistry, Pusan National University) ;
  • Lee, Na-Ri (Department of Life Science and Environmental Biochemistry, Pusan National University) ;
  • Jeong, Seong-Yun (Department of Medical Life Science, Catholic University of Daegu) ;
  • Park, Geun-Tae (Research & University-Industry Cooperation, Pusan National University) ;
  • Son, Hong-Joo (Department of Life Science and Environmental Biochemistry, Pusan National University)
  • 고태훈 (부산대학교 생명환경화학과) ;
  • 정진하 (부산대학교 생명환경화학과) ;
  • 이나리 (부산대학교 생명환경화학과) ;
  • 정성윤 (대구가톨릭대학교 의생명과학과) ;
  • 박근태 (부산대학교 산학협력단) ;
  • 손홍주 (부산대학교 생명환경화학과)
  • Received : 2011.12.05
  • Accepted : 2012.01.20
  • Published : 2012.02.29

Abstract

We isolated and characterized novel duck feather-degrading bacteria producing keratinase. Twelve strains were isolated from soil and faces at poultry farm, and decayed feathers. They were identified as Bacillus methylotrophicus, Pseudomonas geniculata, Pseudomonas hibiscicola, Exiquobacterium profundum, Bacillus pumilus, Bacillus amyloliquefaciens, Chryseobacterium indologenes, Bacillus thuringiensis, Thermomonas koreensis, respectively, by phenotypic characters and 16S rRNA gene analysis. Generally, the level of keratinase production was not proportional to feather degradation rate. The highest keratinolytic activity was observed in the culture inoculated with Chryseobacterium indologenes D27. Although all strains did not degrade human hair, strains tested effectively degraded chicken feather(53.8-91.4%), wool(40.4-93.0%) and human nail (51.0-82.9%). These results suggest that strains isolated could be not only used to improve the nutritional value of recalcitrant feather waste but also is a potential candidate for biotechnological processes of keratin hydrolysis.

Keywords

Feather degradation;Keratin;Keratinase;Wool

Acknowledgement

Supported by : 한국연구재단

References

  1. Bradford, M. M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  2. Brandelli, A., 2008, Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond, Food Bioprocess Technol., 1, 105-116. https://doi.org/10.1007/s11947-007-0025-y
  3. Gerhardt, P., Murray, R. G. E., Costilow, R. N., Wester, E. W., 1981, Manual of methods for general bacteriology, American Society for Microbiology, New York.
  4. Gupta, R., Beg, Q. K., Khan, S., Chauhan, B., 2002, An overview on fermentation, downstream processing and properties of microbial alkaline proteases, Appl. Microbiol. Biotechnol., 60, 381-395. https://doi.org/10.1007/s00253-002-1142-1
  5. Gupta, R., Ramnani, P., 2006, Microbial keratinases and their prospective applications: an overview, Appl. Microbiol. Biotechnol., 70, 21-33. https://doi.org/10.1007/s00253-005-0239-8
  6. Infante, I., Morel, M. A., Ubalde, M. C., Martínez- Rosales, C., Belvisi, S., Castro-Sowinski, S., 2010, Wool-degrading Bacillus isolates: extracellular protease production for microbial processing of fabrics, World J. Microbiol. Biotechnol., 26, 1047-1052. https://doi.org/10.1007/s11274-009-0268-z
  7. Jeong, J. H., Lee, N. R., Kim, J. D., Jeon, Y. D., Park, K. H., Oh, D. J., Lee, C. Y., Son, H. J., 2010, Isolation and characterization of feather keratindegrading bacteria and plant growth-promoting activity of feather hydrolysate, J. Environ. Sci., 19, 1307-1314. https://doi.org/10.5322/JES.2010.19.10.1307
  8. Kang, S. M., Cha, M. K., Kim, S. J., Kwon, Y. J., 2006, The effect of quality improvement for wool and silk treated with protease produced by B. subtilis K-54, J. Kor. Soc. Cloth. Ind., 8, 239-244.
  9. Karthikeyan, R., Balaji, S., Sehgal, P. K., 2007, Industrial applications of keratins- a review, J. Sci. Ind. Res., 66, 710-715.
  10. Kaul, S., Sumbali, G., 1999, Production of extracellular keratinase by keratinophilic fungal species inhabiting feathers of living poultry birds (Gallus domesticus): A comparison, Mycopathologia, 146, 19-24. https://doi.org/10.1023/A:1007086720237
  11. Kornillowicz-Kowalska, T., Bohacz, J., 2011, Biodegradation of keratin waste: theory and practical aspects, Waste Management, 31, 1689-1701. https://doi.org/10.1016/j.wasman.2011.03.024
  12. Lane, D. J., 1991, 16S/23S rRNA sequencing, in: Stackebrandt, E., Goodfellow, M. (eds.), Nucleic acid techniques in bacterial systematics, John Wiley and Sons, New York, 115-175.
  13. Langeveld, J. P. M., Wang, J. J., Van de Wiel, D. F. M., Shih, G. C., Garssen, G. J., Bossers, A., Shih, J. C. H., 2003, Enzymatic degradation of prion protein in brain stem from infected cattle and sheep, J. Infect. Dis., 188, 1782-1789. https://doi.org/10.1086/379664
  14. Lin, X., Lee, C. C., Csale, E. S., Shih, J. C. H., 1992, Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain, Appl. Environ. Microbiol., 58, 3271-3275.
  15. Onifade, A. A., Al-Sane, N. A., Al-Musallam, A. A., Al-Zarban, S., 1998, Potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources, Biores. Technol., 66, 1-11. https://doi.org/10.1016/S0960-8524(98)00033-9
  16. Papadoulos, M. C., El Boushy, A. R., Roodbeen, A. E., Ketelaars, E. H., 1986, Effects of processing time and moisture content on amino acid composition and nitrogen characteristics of feather meal, Anim. Feed Sci. Technol., 14, 279-290. https://doi.org/10.1016/0377-8401(86)90100-8
  17. Park, G. T., Son, H. J., 2009, Keratinolytic activity of Bacillus megaterium F7-1, a feather-degrading mesophilic bacterium, Microbiol. Res., 164, 478-485. https://doi.org/10.1016/j.micres.2007.02.004
  18. Rao, M. B., Tanksale, A. M., Ghatge, M. S., Deshpande, V. V., 1998, Molecular and biotechnological aspects of microbial proteases, Microbiol. Mol. Biol. Rev., 62, 597-635.
  19. Singh, C. J., 2002, Optimization of an extracellular protease of Chrysosporium keratinophilum and its potential in bioremediation of keratinic wastes, Mycophathologia, 156, 151-156.
  20. Son, H. J., Park, G. T., Kim, Y. G., 2004, Production of a keratinolytic protease by a feather-degrading bacterium, Bacillus megaterium F7-1, Kor. J. Microbiol., 40, 43-48.
  21. Wawrzkiewicz, K., Lobarzewski, J., Wolski, T., 1987, Intracellular keratinase of Trichophton gallinae, J. Med. Vet. Mycol., 25, 261-268. https://doi.org/10.1080/02681218780000601

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