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Effect of Radiation Dose for Radiotherapy on Ovarian Follicle Atresia in Rat

치료 방사선량이 쥐의 난포 퇴축에 미치는 영향

  • Lee, Won-Jeong (Occupational Lung Diseases Institute, KCOMWEL) ;
  • Seon, Jong-Ryul (Department of Radiological Technology, Daejeon Health Science College) ;
  • Yoo, Se-Jong (Department of Diagnostic Radiology, Konyang University Hospital) ;
  • Ahn, Bong-Seon (Department of Radiological Technology, Daejeon Health Science College)
  • 이원정 (근로복지공단 직업성폐질환연구소) ;
  • 선종률 (대전보건대학교 방사선과) ;
  • 유세종 (건양대학교병원 영상의학과) ;
  • 안봉선 (대전보건대학교 방사선과)
  • Received : 2012.11.08
  • Accepted : 2012.12.12
  • Published : 2012.12.31

Abstract

In previous studies, ovarian follicle in rat has been used a higher radiation dose than that for cancer radiotherapy in clinical practice. The aim of this study was to evaluate the effect of radiation dose used for cancer radiotherapy on ovarian follicle atresia in rat. Mice of 4-week-old female were whole body irradiated with 2 cGy or 2 Gy (Mevatron 67, Siemens, Germany) and sacrificed by cervical dislocation. Ovaries were collected at 24 hours after irradiation to observe the degree of follicular atresia. Ovaries were fixed in neutral formaldehyde solution for 24 hours and embedded with paraffin. Cutted in $5{\mu}m$ thickness with microtome and stained with hematoxylin and eosin (H&E) and TUNEL immunohistochemical stain, and examined histologically under a light microscope. All data were presented as mean ${\pm}SD$, calculating the ratio of normal or atretic follicles to total ovarian follicles. Statistical analysis was performed by the Mann Whitney test using the SPSS ver 19.0. Ratio of atretic to total follicles of 2 Gy group was significantly higher than control or 2 cGy groups (p<0.05). Ratio of normal to total follicles of 2 Gy group was significantly lower than control group in preantral follicle (64.0 vs. 87.7, p=0.027). Ratio of normal to total follicles of 2 cGy group was significantly increased more than control or 2 Gy groups in antral follicle, and there were no significant difference between control and 2 Gy groups (p=0.522). Radiation dose of 2 Gy for cancer radiotherapy have a significant effect on ovarian follicle atresia in rat.

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