Fibrinolytic Activity and Chemical Properties of Cordycepin-Enriched Cordyceps militaris JLM 0636

Cordycepin 고함유 동충하초 수용성 추출물의 이화학적 특성과 혈전 용해활성

  • Received : 2011.12.16
  • Accepted : 2012.02.10
  • Published : 2012.02.28


The effect of cordycepin-enriched Cordyceps militaris JLM 0636 ($CM{\alpha}$) and Cordyceps militaris (CM) on fibrinolytic activity was investigated. The bioactive compounds and nutritional materials such as polyphenolic compounds, flavonoids, glutathione, minerals, and fatty acids were also measured. Concentrations of polyphenol compounds, flavonoids, and glutathione were higher in $CM{\alpha}$ than that in CM. The major minerals of both materials were K, Ca, Mg, and Na. The major fatty acids of both materials were linolenic acid, linoleic acid, oleic acid, and palmitic acid. Fibrinolytic activity was higher in $CM{\alpha}$ than that in CM. These results may provide the basic data to understand the fibrinolytic activity and bioactive compounds of $CM{\alpha}$.


Cordyceps militaris;cordycepin;fibrinolytic activity;glutathione


  1. A.O.A.C. 1975. Official methods of analysis. 12th eds., Association of official analytical chemists. Washington, D.C., U.S.A.
  2. Astrup, T. and S. Mullertz. 1991. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40, 346-351.
  3. Butenas, S. and S. Mullertz. 2002. Blood coagulation. Biochemistry (Moscow) 67, 3-12.
  4. Cha, J. Y. and Y. S. Cho. 1999. Effect of potato polyphenolics on lipid peroxidation in rats. J. Korean Soc. Food Sci. Nutr. 28, 1131-1136.
  5. Cha, J. Y., B. K. Park, K. E. Eom, H. Y. Ahn, and Y. S. Cho. 2009. Effect of Glutathione-Enriched Saccharomyces cerevisiae FF-8 on Tissues Lipid Peroxidation in Orotic Acid-Induced Fatty Liver Model Rats. J. Life Sci. 19, 322-326.
  6. Cha, J. Y., H. Y. Ahn, S. J. Heo, M. J. Kang, J. H. Lee, S. H. Park, Y. K. Jeong, and Y. S. Cho. 2011. Improvement effect of cordycepin-enriched Cordyceps militaris JLM 0636 powder against orotic acid-induced fatty liver in rats. J. Life Sci. 21, 1274-1280.
  7. Cha, J. Y., H. I. Moon, H. Y. Ahn, S. J. Heo, and Y. S. Cho. 2011. Comparative effects of Paecilomyces japonic, Cordyceps militaris, cordycepin3 enriched Cordyceps militaris against antioxidant and hypolipidemia in alcohol feeding Sprague-Dawley rats. Immunopharmacology & Immunotoxicology 2011 in press.
  8. Cho, H. J., J. Y. Cho, M. H. Rhee, H. S. Kim, H. S. Lee, and H. J. Park. 2007. Inhibitory effects of cordycepin (3'-deoxyadenosine), a component of Cordyceps militaris, on human platelet aggregation induced by thapsigargin. J. Microbiol. Biotechnol. 17, 1134-1138.
  9. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42.
  10. Eikelboom, J. W., S. L. Zelenkofske, and C. P. Rusconi. 2010. Coagulation factor IXa as a target for treatment and prophylaxis of venous thromboembolism. Arterioscler. Thromb. Vasc. Biol. 30, 382-387.
  11. Fahey, R. C. and G. I. Newton. 1983. Eutamoeba historlytica: A Eukaryote without glutathione metabolism. Science 224, 70-72.
  12. Guo, P., Q. Kai, J. Gao, Z. Q. Lian, C. M. Wu, C. A. Wu, and H. B. Zhu. 2010. Cordycepin prevents hyperlipidemia in hamsters fed a high-fat diet via activation of AMP-activated protein kinase. J. Pharmacol. Sci. 113, 395-403.
  13. Han, E. K., Y. X. Jin, Y. S. Yoo, E. J. Jung, J. Y. Lee, and C. K. Chung. 2009. Effect of Artemisia capillaris and Paecilomyces japonica on the Reduction of Hepatotoxicity and Lipid Metabolism Induced by Ethanol. J. Korean Soc. Food Sci. Nutr. 38, 1016-1023.
  14. Ji, D. B, J. Ye, C. L. Li, Y. H. Wang, J. Zhao, and S. Q. Cai. 2009. Antiaging effect of Cordyceps sinensis extract. Phytother. Res. 23, 116-122.
  15. Jia, Z., M. Tang, and J. Wu. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem. 64, 555-559.
  16. Kenzo, Y., T. Hirashi, and H. Yoshiteru. 1985. Glutathione no seizouho. Japan Patent 60-160894.
  17. Kim, H. K., Y. J. Choi, and K. H. Kim. 2002. Functional activities of microwave-assisted extracts from Flammulina velutipes. Korean J. Food Sci. Technol. 34, 1013-1017.
  18. Kim, H. K., Y. J. Choi, S. W. Jeong, K. H. Kim. 2002. Functional activities of microwave-assisted extracts from Lyophyllum ulmarium. Korean J. Food Preserv 9, 385-390.
  19. Kim, E. Y., I. H. Baik, J. H. Kim, S. R. Kim, and M. R. Rhyu. 2004. Screening of the antioxidant activity of some medicinal plants. Korean J. Food Sci. Technol. 36, 333-338.
  20. Li, S. P., G. H. Zhang, Q. Zeng, Z. G. Huang, Y. T. Wang, T. T. Dong, and K. W. Tsim. 2006. Hypoglycemic activity of polysaccharide, with antioxidation, isolated from cultured Cordyceps mycelia. Phytomedicine 13, 428-433.
  21. Lie, C. H., M. T. Huang, and P. C. Huang. 1995. Sources of triglyceride accumulation in liver of rats fed a cholesterol-supplemented diet. Lipids 30, 527-532.
  22. Muller, W. E., B. E. Weiler, R. Charubala, W. Pfleiderer, L. Leserman, R. W. Sobol, R. J. Suhadolnik, and H. C. Schroder. 1991. Cordycepin analogues of 2', 5'-oligo-adenylate inhibition of reverse transcriptase. Biochemistry 30, 2027-2033.
  23. Murata, K. and A. Kimura. 1986. Relationship between glutathione contents and generation times in Saccaromyces cerevisiae. Agric. Biol. Chem. 50, 1055-1056.
  24. Ok, M. and Y. S. Cho. 2005. Screening of fibrinolytic enzyme producing from microorganism in Korean fermented soybean paste and optimum conditions of enzyme production. Korean J. Food Preserv 12, 643-649.
  25. Owens, C. W. I. and R. V. Belcher. 1965. A colorimetric micro-method for the determination of glutathione. Biochem. J. 94, 705-711.
  26. Sohn, H. Y., Y. S. Kwon, Y. S. Kim, H. Y. Kwon, G. S. Kwon, G. J. Kim, C. S. Kwon, and K. H. Son. 2004. Screening of thrombin inhibitors from medicinal and wild plants. Korean J. Pharmacogn. 35, 52-61.
  27. Sugimura, Y. and K. Yamamoto. 1998. The protective effect of glutathione-enriched yeast extract on acetaminophen-induced liver damage in rats. J. Jpn. Soc. Nutr. Food Sci. 51, 189-193.
  28. Swain, T., W. E. Hillis and M. Oritega. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agric. 10, 83-88.
  29. Voet, D. and J. G. Voet. 1990. Biochemistry, John Wiley & Sons, New York. 9, 1087-1095.
  30. Zhang, H. X., W. Wu, W. Chen, X. H. Gao, and L. S. Tang. 2005. Analysis of cordycepin and adenosine contents in fermentation supernatant of Cordyceps millitaris by HPLC. Acta Agriculture Shanghai 21, 53-56.

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