Effects of Replacement of Soybean Meal by Fermented Cottonseed Meal on Growth Performance, Serum Biochemical Parameters and Immune Function of Yellow-feathered Broilers

  • Tang, J.W. (Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Sciences) ;
  • Sun, H. (College of Animal Sciences, Zhejiang University) ;
  • Yao, X.H. (Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Sciences) ;
  • Wu, Y.F. (Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Sciences) ;
  • Wang, X. (Institute of Plant Protection and Microbiology, Zhejiang Academy of Agriculture Sciences) ;
  • Feng, J. (College of Animal Sciences, Zhejiang University)
  • Received : 2011.10.21
  • Accepted : 2011.12.27
  • Published : 2012.03.01


The study was conducted to examine the effects of partially replacing soybean meal (SBM) by solid-state fermented cottonseed meal (FCSM) on growth performance, serum biochemical parameters and immune function of broilers. After inoculated with Bacillus subtilis BJ-1 for 48 h, the content of free gossypol in cottonseed meal was decreased from 0.82 to 0.21 g/kg. A total of 600, day-old male yellow-feathered broilers were randomly divided into four groups with three replicates of 50 chicks each. A corn-SBM based control diet was formulated and the experimental diets included 4, 8 or 12% FCSM, replacing SBM. Throughout the experiment, broilers fed 8% FCSM had higher (p<0.05) body weight gain than those fed 0, 4 and 12% FCSM. The feed intake in 8% FCSM group was superior (p<0.05) to other treatments from d 21 to 42. On d 21, the concentration of serum immunoglobin M in the 4% and 8% FCSM groups, as well as the content of complements (C3, C4) in 8% FCSM group were greater (p<0.05) than those in the SBM group. Besides, birds fed 8% FCSM had increased (p<0.05) serum immunoglobin M, immunoglobulin G and complement C4 levels on d 42 compared with bird fed control diet. No differences (p>0.05) were found between treatments regarding the serum biochemical parameters and the relative weights of immune organs. In conclusion, FCSM can be used in broiler diets at up to 12% of the total diet and an appropriate replacement of SBM with FCSM may improve growth performance and immunity in broilers.


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