The Signaling Mechanism of TGF-β1 Induced Bovine Mammary Epithelial Cell Apoptosis

  • Di, He-Shuang (Department of Animal Science and Technology, Nanjing Agricultural University) ;
  • Wang, Li-Gang (Jiangsu Animal Husbandry and Veterinary College) ;
  • Wang, Gen-Lin (Department of Animal Science and Technology, Nanjing Agricultural University) ;
  • Zhou, Lei (Department of Animal Science and Technology, Nanjing Agricultural University) ;
  • Yang, Yuan-Yuan (Department of Animal Science and Technology, Nanjing Agricultural University)
  • Received : 2011.07.28
  • Accepted : 2011.11.13
  • Published : 2012.03.01


The present study showed that Transforming growth factor beta 1 (TGF-${\beta}_1$) can induce apoptosis of bovine mammary epithelial cells. This apoptosis was also observed with phosphorylation of Smad2/3 within 0.5-2 h. Afterwards the signal transferred into the nucleus. Moreover, intracellular free $Ca^{2+}$ concentration was significantly elevated as well as Caspase-3 activated and DNA lysised, thereby inducing the programmed cell death. This signaling pathway of TGF-${\beta}_1$ was blocked by SB-431542 ($10^{-2}{\mu}M$) via inhibiting ALK-5 kinase activity, which thus reversed the anti-proliferation and apoptosis effect of TGF-${\beta}_1$ in mammary epithelial cells. These results indicated that TGF-${\beta}_1$ induced apoptosis of bovine mammary epithelial cells through the ALK-5-Smad2/3 pathway, which plays an important role in inhibiting survival of mammary epithelial cells. Moreover, intracellular $Ca^{2+}$ also played a critical role in TGF-${\beta}_1$-induced cell apoptosis.


TGF-${\beta}_1$;ALK-5;p-Smad2/3;[$Ca^{2+}$]i;Mammary Epithelial Cell


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