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Detection Method for Identification of Pueraria mirifica (Thai kudzu) in Processed Foods

가공식품 중 태국칡(Pueraria mirifica) 혼입 판별법 개발

  • Park, Yong-Chjun (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Jin, Sang-Wook (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Kim, Mi-Ra (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Kim, Kyu-Heon (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Lee, Jae-Hwang (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Cho, Tae-Yong (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Lee, Hwa-Jung (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Lee, Sang-Jae (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration) ;
  • Han, Sang-Bae (Scientific Food Investigation Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Food & Drug Administration)
  • 박용춘 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 진상욱 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 김미라 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 김규헌 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 이재황 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 조태용 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 이화정 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 이상재 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀) ;
  • 한상배 (식품의약품안전청 식품의약품안전평가원 식품위해평가부 식품감시과학팀)
  • Received : 2012.05.03
  • Accepted : 2012.12.03
  • Published : 2012.12.31

Abstract

In this study, ribulose bisphosphate carboxylase (rbcL), RNApolymeraseC (rpoC1), intergenic spacer (psbA-trnH), and second internal transcribed spacer (ITS2) as identification markers for discrimination of P. mirifica in foods were selected. To be primer design, we obtained 719 bp, 520 bp, 348 bp, and 507 bp amplicon using universal primers from selected regions of P. mirifica. The regions of rbcL, rpoC1, and psbA-trnH were not proper for design primers because of high homology about P. mirifica, P. lobata, and B. superba. But, we had designed 4 pairs of oligonucleotide primers from ITS2 gene. Predicted amplicon from P. mirifica were obtained 137 bp and 216 bp using finally designed primers SFI12-miri-6F/SFI12-miri-7R and SFI12-miri-6F/SFI12-miri-8R, respectively. The species-specific primers distinguished P. mirifica from related species were able to apply food materials and processed foods. The developed PCR method would be applicable to food safety management for illegally distributed products in markets and internet shopping malls.

Keywords

PCR (Polymerase Chain Reaction);Pueraria mirifica;species-specific primer

Acknowledgement

Supported by : 식품의약품안전평가원

References

  1. Kim, J.S., Kim, D.K., Jang, D.S., and Kim, J.H.: Genetic variation and phylogenetic relationship of and pueraria lobata ohwi (fabaceae) and related taxa by RPAD markers. Korean J. Plant Res, 22, 446-453 (2009).
  2. Koichi, T. and Hideji, I.: Isoflavonoids and the other constituents in callus tissues of pueraria lobata. Chem. Pharm. Bull, 30, 1496-1499 (1982). https://doi.org/10.1248/cpb.30.1496
  3. Junei, K., Juncihi, F., Junko, B., Takashi, T. and Masaki, Y.: Studies on the constituents of pueraria lobata. Chem. Pharm. Bull, 35, 4846-4850 (1987). https://doi.org/10.1248/cpb.35.4846
  4. Takashi, S., Akihiko, K., Akira, T., Yoshio, T. and Tatsuzo, F.: Mechanism of fantioxidants action of pueraria glycoside (PG)- 1 (an isoflavonoids) and mangiferin (a Zanthonoid). Chem. Pharm. Bull, 40, 721-724 (1992). https://doi.org/10.1248/cpb.40.721
  5. Chandeying, V. and Lamlertkittikul, S.: Challenges in the conduct of thai herbal scientific study: efficacy and safety of phytoestrogen, pueraria mirifica(kwao keur kao), phase1, in the alleviation of climacteric symptoms in perimenopausal women. J. Med. Assoc. Thai, 90, 1274-1280 (2007).
  6. Lee, Y.S., Park, J.S., Co, S.D., Son, J.K., Cherdshewasart, W. and Kang, K.S.: Requirement of Metabolic activation for estrogenic activity of pueraria mirifica. J. Vet. Sci, 3, 273-277 (2002).
  7. Virojchaiwong, P., Suvithayasiri, V. and Itharat, A.: Comparison of pueraria mirifica 25 and 50 mg for menopausal symptoms. Arch. Gynecol. Obstet, 284, 411-419 (2011). https://doi.org/10.1007/s00404-010-1689-5
  8. Jaroenporn, S., Malaivijitnond, S., Wattanasiirmkit, K., Trisomboon, H., Watanabe, G., Taya, K. and Cherdshewasart, W.: Effects of pueraria mirifica, an herb containing phytoestrogens, on reproductive organs and fertility of adult male mice. Endocr. J, 30, 93-101 (2006). https://doi.org/10.1385/ENDO:30:1:93
  9. Manonai, J., Chittacharoen, A., Theppisai, U. and Theppisai, H.: Effect of pueraria mirifica on vaginal health. Menopause, 14, 919-924 (2007). https://doi.org/10.1097/gme.0b013e3180399486
  10. 식품의약품안전청: 식품공전, 별표 3 (2011).
  11. Padovan, G.J., Jong, D.D., Rodrigues, L.P. and Marchini, J.S.: Detection of adulteration of commercial honey samples by the 13C/12C isotope ratio. Food Chem, 82, 633-636 (2003). https://doi.org/10.1016/S0308-8146(02)00504-6
  12. Schellenberg, A., Chmielus, S., Schlicht, C., Camin, F., Perini, M., Bontempo, L., Heinrich, K., Kelly, S.D., Rossmann, A., Thomas, J. and Horacek, M.: Multielement stable isotope ratio(H, C, N, S) of honey from different european regions. Food Chem, 121, 770-777 (2010). https://doi.org/10.1016/j.foodchem.2009.12.082
  13. Williams, J.G.K., Kubelik, A.R., Livark, K.J., Rafalski, J.A. and Tingey, S.V.: DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic. Acids. Res, 18, 6531-6535 (1990). https://doi.org/10.1093/nar/18.22.6531
  14. Bunmanop, S., Sakuanrungsirikul, S. and Manakasem, Y.: White kwao krua variety classification by botanical characteristics and ISSR-Touchdown PCR technique. Russ. J. Genet, 47, 819-828 (2011). https://doi.org/10.1134/S1022795411070039
  15. Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R.: DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotech, 3, 294-299 (1994).
  16. Herbert, P.D.N., Retnasingham, S. and deWaard, J.R.: Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proc. R. Soc. Lond. B, 270, S96-S99 (2003). https://doi.org/10.1098/rsbl.2003.0025
  17. Hebert, P.D.N. Cywinska, A., Shelley L.B. and deWaard, J.R.: Biological identifications through DNA Barcodes. Proc. R. Soc. Lond. B, 270, 313-321 (2003). https://doi.org/10.1098/rspb.2002.2218
  18. Chen, S. and Song, J.: Validation of the ITS2 region as a Novel DNA barcode for identifying medicinal plant species. PLoS One, 5, e8613. dol:10.1371 (2010). https://doi.org/10.1371/journal.pone.0008613
  19. Chen, S., Yao, H., Han, J., Liu, C., Song, J., Shi, L. and Zhu, Y.: Validation of the ITS2 region as a Novel DNA barcode for identifying medicinal plant species. PLoS one. 5, e8613-8620 (2010). https://doi.org/10.1371/journal.pone.0008613
  20. Hsieh, H.M., Chiang, H.L., Tsai, L.C., Lai, S.Y., Huang, N.E., Linacre, A. and Lee, J.C.: Cytochrome b gene for species identification of the conservation animals. Forensic Sci. Int, 122, 7-18 (2001). https://doi.org/10.1016/S0379-0738(01)00403-0
  21. Wolstenholme, D.R.: Animal mitochondrial DNA: structure and evolution. Int. Rev. Cytol, 141, 173-216 (1992). https://doi.org/10.1016/S0074-7696(08)62066-5
  22. Chen, S. and Song, J.: Validation of the ITS2 region as a Novel DNA barcode for identifying medicinal plant species. PLos One, 5, e8613. dol:10.1371 (2010). https://doi.org/10.1371/journal.pone.0008613
  23. Fay, M.F., Bayer, C., Alverson, W.S., deBruijin, A.Y. and Chase, M.W.: Plastid rbcL sequence data indicate a close affinity between Diegodendron and Bixa. Taxon, 47, 43-50 (1998). https://doi.org/10.2307/1224017
  24. Fazekas, A.J., Burgess, K.S., Kesankurti, P.R., Graham, S.W., Newmaster, S.G. and Husband, B.C.: Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PloS ONE, 3, e2802 (2008). https://doi.org/10.1371/journal.pone.0002802
  25. Newmaster, S.G. and Ragupathy, S.: Testing plant barcoding in a sister species complex of pantropical Acacia (Mimosoidea, Fabaceae). Mol. Ecol. Res, 9, 172-180 (2009). https://doi.org/10.1111/j.1755-0998.2009.02642.x
  26. Gray, M.W., Cedergren, R.Y. and Sankoff, A.D.: On the evolutionary origin of the plant mitochondrion and its genome. Proc. Natl. Acad. Sci, 86, 2267-2271 (1989). https://doi.org/10.1073/pnas.86.7.2267
  27. Chung, J.W., Kim, G.Y., Ha, M.G., Lee, T.H. and Lee, J.D.: Phylogenetic analysis of the genus phellinus by comparing the sequences of internal transcribed spacers and 5.8S ribosomal DNA. Korean J. Mycol, 27, 124-131 (1999).
  28. Park, D.S., Go, S.J. and Ryu, J.C.: Phylogenetic relationships of coprinoid taxa and an agaric-like gastrois taxon based on the sequence of internal transcribed spacer (ITS) regions. Korean J. Mycol, 27, 406-411 (1999).

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