DOI QR코드

DOI QR Code

Pretreatment Effect of Running Exercise on HSP70 and DOX-Induced Cardiotoxicity

Shirinbayan, Vahid;Roshan, Valiollah Dabidi

  • Published : 2012.11.30

Abstract

Objective: The purpose of this study was to determine pretreatment effects of moderate-term endurance training before the various dosages (10 and $20{_{mg.kg}}^{-1}$) of DOX on a heat shock protein ($HSP_{70kda}$) and cardiotoxicity in heart tissue. Methods: Forty-eight male rats were randomly assigned to nontraining (NT) and training (T) groups and three subgroups; $DOX{_{10mg.kg}}^{-1}$ and $DOX{_{20mg.kg}}^{-1}$ and saline treatment. The training program included treadmill running between 25-39 min/day and 15-17 m/min, 5 days/wk for 3 wk. Result: DOX administration, in particularly with $20{_{mg.kg}}^{-1}$, caused up-regulation of oxidants and cardiac damage (MDA, CK, CPK-MB and CK/CPK-MB) and down-regulation of cardioprotection ($HSP_{70}$, SOD) markers, as compared to NT+saline group. Pretreatment effect of treadmill running endurance exercise in the presence of DOX with $10{_{mg.kg}}^{-1}$ caused a significant increase in $HSP_{70}$, SOD and a significant decrease in MDA and insignificant decrease in CK, CPK-MB and CK/CPK-MB, in comparison $T+DOX_{10}$ with $NT+DOX_{10}$ group. However, there was no significant difference between $T+DOX{_{10mg.kg}}^{-1}$ and $T+DOX{_{20mg.kg}}^{-1}$ in the aforesaid markers. Conclusion: Dox-induced cardiotoxicity is related to oxidative stress. Our study suggests that pretreatment with endurance exercise may be considered as a potentially useful strategy to improve myocardial tolerance against single dose DOX-induced oxidative damage.

Keywords

Doxorubicin;cancer;cardiotoxicity;endurance exercise

References

  1. Ammar EL-SM, Said SA, Suddek GM, et al (2011). Amelioration of doxorubicin-induced cardiotoxicity by deferiprone in rats. Can J Physiol Pharmacol, 89, 269-76. https://doi.org/10.1139/y11-020
  2. Ascensao A, Magalhaes J, Soares JM, et al (2005). Neuparth, FM, Paulo J. Oliveira, and Duarte J A. Moderate endurance training prevents doxorubicin-induced in vivo mitochondriopathy and reduces the development of cardiac apoptosis. Am J Physiol Heart Circ Physiol, 289, 722-31. https://doi.org/10.1152/ajpheart.01249.2004
  3. Ascensao A, Magalhaes J, Soares J, et al (2005). Endurance training attenuates doxorubicin induced cardiac oxidative damage in mice. Int J Cardiol, 100, 451-60. https://doi.org/10.1016/j.ijcard.2004.11.004
  4. Ascensao A, Oliveira PJ, Magalhaes J (2011). Exercise as a beneficial adjunct therapy during Doxorubicin treatment-Role of mitochondria in cardioprotection. Int J Cardiology, 156, 4-10.
  5. Babaei R, Ejtemaeemehr S, Tavangar S, et al (2008). Morphine is protective against doxorubicin-induced cardiotoxicity in rat. Toxicology, 243, 96-10. https://doi.org/10.1016/j.tox.2007.09.026
  6. Bayer ML, Paulsen G, Ugelstad I, et al (2006). Relocalization and upregulation of HSP70 after muscle damaging eccentric exercise. 11th annual congress of the European College of Sport Science, Lausanne/Switzerland, July 5th-8th.
  7. Bulduk E, Ergene N, Baltaci A, et al (2011). Plasma antioxidant responses and oxidative stress following a 20 meter shuttle run test in female volleyball players. Int J Human Sci, 8, 510-26.
  8. Chicco A, Hydock D, Schneider, et al (2006). Low-intensity exercise training during doxorubicin treatment protects against cardiotoxicity. J Appl Physiol, 100, 519-27. https://doi.org/10.1152/japplphysiol.00148.2005
  9. Chicco AJ, Schneider CM, Hayward R (2005). Voluntary exercise protects against acute doxorubicin cardiotoxicity in the isolated perfused rat heart. Am J Physiol Regul Integr Comp Physiol, 289, 424-43. https://doi.org/10.1152/ajpregu.00636.2004
  10. Chicco AJ, Schneider CM, Hayward R (2006). Exercise training attenuates acute doxorubicin-induced cardiac dysfunction. J Cardiovasc Pharmacol, 47, 182-9. https://doi.org/10.1097/01.fjc.0000199682.43448.2d
  11. Dabidi Roshan Va, Assali M, Hajizadeh Moghaddam A, et al (2011). Exercise training and antioxidants: effects on rat heart tissue exposed to lead acetate. Int J Toxicology, 30, 190-6. https://doi.org/10.1177/1091581810392809
  12. Dabidi Roshan Vb, Ranjbar S, Hosseinzadeh M, et al (2011). Left ventricular oxidant and antioxidant markers induced by lifestyle modification in rats exposed to lead acetate. Eur J Sport Sci, 1-6,.
  13. Douglas K Bowles (2008). Curing the cure: utilizing exercise to limit cardiotoxicity. Med Sci Sports Exerc, 40, 806-7. https://doi.org/10.1249/MSS.0b013e3181684d03
  14. Feairheller D, Diaz K, Sturgeon K, et al (2011). Racial Differences in the Time-Course Oxidative Stress Responses to Acute Exercise. J Exercise Physiology, 14, 49-59.
  15. Fei Xin Y, Wan L, Peng J, et al (2011). Alleviation of the acute doxorubicin-induced cardiotoxicity by Lycium barbarum polysaccharides through the suppression of oxidative stress. Food and Chemical Toxicology, 49, 259-64. https://doi.org/10.1016/j.fct.2010.10.028
  16. Hydock DS, Lien CY, Jensen BT, et al (2011). Exercise preconditioning provides long-term protection against early chronic doxorubicin cardiotoxicity. Integr Cancer Ther, 10, 47-57.
  17. Hydock DS, Lien CY, Schneider CM, et al (2008). Exercise preconditioning protects against doxorubicin induced cardiac dysfunction. Med Sci Sports Exerc, 40, 808-17. https://doi.org/10.1249/MSS.0b013e318163744a
  18. Jakovljevic V, Zlatkovic M, Cubrilo D, et al (2011). The effects of progressive exercise on cardiovascular function in elite athletes: Focus on oxidative stress. Acta Physiologica Hungarica, 1, 51-8.
  19. Jovanka K, Mira P, Momir M, et al (2009). Protective Effects of Celery Juice in Treatments with Doxorubicin. Molecules, 14, 1627-38. https://doi.org/10.3390/molecules14041627
  20. Kavazis A, Smuder A, Min K, et al (2010). Powers. Short-term exercise training protects against doxorubicin-induced cardiac mitochondrial damage independent of HSP72. Am J Physiol Heart Circ Physiol, 299, 1515-24. https://doi.org/10.1152/ajpheart.00585.2010
  21. Kavazis AN (2009). Exercise preconditioning of the myocardium. Sports Med, 39, 923-35. https://doi.org/10.2165/11317870-000000000-00000
  22. Kruk J (2011). Physical exercise and oxidative stress. Med Sport, 15, 30-40. https://doi.org/10.2478/v10036-011-0004-2
  23. Menna P, Salvatorelli E, Minotti G (2010). Anthracycline degradation in cardiomyocytes: a journey to oxidative survival. Chem Res Toxicol, 23, 6-10. https://doi.org/10.1021/tx9003424
  24. Ogawa K, Seta R, Shimizu T, et al (2011). Plasma adenosine triphosphate and heat shock protein 72 concentrations after aerobic and eccentric exercise. Tokyo Metropolitan Institute of Gerontology EIR, 17, 136-49.
  25. Powers SK, Locke AM, Demirel HA (2001). Exercise, heat shock proteins, and myocardial protection from I-R injury. Med Sci Sports Exerc, 33, 386-92. https://doi.org/10.1097/00005768-200103000-00009
  26. Rade I, Martina P, Natasa O, et al (2008). Potential hepatoprotective effects of fullerenol C60 (OH) 24 in doxorubicin-induced hepatotoxicity in rats with mammary carcinomas. Biomaterials, 29, 3451-60. https://doi.org/10.1016/j.biomaterials.2008.04.048
  27. Raskovic A, Stilinovic N, Kolarovic J, et al (2011). The protective effects of silymarin against doxorubicin-induced cardiotoxicity and hepatotoxicity in rats. Molecules, 16, 8601-13. https://doi.org/10.3390/molecules16108601
  28. Rios-Doria J, Carie A, Costich T, et al (2012). A versatile polymer micelle drug delivery system for encapsulation and in vivo stabilization of hydrophobic anticancer drugs. J Drug Deliv, 951741.
  29. Trapp D, Knez W, Sinclair W (2010). Could a vegetarian diet reduce exercise-induced oxidative stress? A review of the literature. J Sports Sci, 28, 1261-8. https://doi.org/10.1080/02640414.2010.507676
  30. Visw Anatha A, Sumeet G, Thippeswamy A, et al (2012). Cardioprotective effect of curcumin against doxorubicininduced myocardial toxicity in albino rats. Indian J Pharmacology, 44, 73-7. https://doi.org/10.4103/0253-7613.91871
  31. Wonders KY, Hydock DS, Schneider CM, et al (2008). Acute exercise protects against doxorubicin cardiotoxicity. Integr Cancer Ther, 7, 147-54. https://doi.org/10.1177/1534735408322848
  32. Wouters KA, Kremer LCM, Miller TL, et al (2005). Protecting against anthracycline-induced myocardial damage: a review of the most promising strategies. Bri J Haematology, 131, 561-78. https://doi.org/10.1111/j.1365-2141.2005.05759.x
  33. Zong-Yan C, Cheng-Chen H, Mei-Chich H, et al (2011). The effect of moderate endurance training on cardioprotective molecule adaptations. Hlth MED, 5, 1912-40.

Cited by

  1. Pretreatment Hepatoprotective Effect of Regular Aerobic Training Against Hepatic Toxicity Induced by Doxorubicin In Rats vol.14, pp.5, 2013, https://doi.org/10.7314/APJCP.2013.14.5.2931
  2. Aerobic exercise in anthracycline-induced cardiotoxicity: a systematic review of current evidence and future directions vol.312, pp.2, 2017, https://doi.org/10.1152/ajpheart.00646.2016
  3. Aerobic Exercise During Early Murine Doxorubicin Exposure Mitigates Cardiac Toxicity vol.40, pp.3, 2018, https://doi.org/10.1097/MPH.0000000000001112
  4. Aerobic exercise and cardiopulmonary fitness in childhood cancer survivors treated with a cardiotoxic agent: a meta-analysis vol.26, pp.7, 2018, https://doi.org/10.1007/s00520-018-4208-z