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Growth Inhibition of Plant Pathogenic Fungi by a Chitinase of Coprinellus congregatus

먹물버섯의 키틴질 분해효소에 의한 식물병원균류의 생장억제

  • Kang, Yuri (Department of Biochemistry, Kangwon National University) ;
  • Choi, Hyoung T. (Department of Biochemistry, Kangwon National University)
  • Received : 2012.12.03
  • Accepted : 2012.12.17
  • Published : 2012.12.31

Abstract

Experiments for growth inhibition of two different plant pathogenic fungal species, Alternaria alternata (KCTC26781) and Fusarium graminearum ZO3639 (type culture) and ASR1R1 (isolate from rice), by a chitinase (Chi2) expressed in the autolysing tissue of Coprinellus congregatus were carried out. In liquid media, Chi2 ($50{\mu}g/ml$) inhibited more than 90% of germination of A. alternata spore, and the growth of each fungal strain was totally inhibited by the addition of Chi2 at the concentrations of $70{\mu}g/ml$. When $6{\mu}g$ of Chi2 was added twice a day at the hyphal tip zone, both strains of F. graminearum showed growth inhibition as well as decreased hyphal branching.

Keywords

Alternaria alternata;Coprinellus congregatus;Fusarium graminearum chitinase

Acknowledgement

Supported by : 한국연구재단

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