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Molecular Divergences of 16S rRNA and rpoB Gene in Marine Isolates of the Order Oscillatoriales (Cyanobacteria)

남조세균 흔들말목(Cyanobacteria, Oscillatoriales) 해양 균주의 16S rRNA와 rpoB 유전자 변이

  • Cheon, Ju-Yong (Department of Green Life Science, Sangmyung University) ;
  • Lee, Min-Ah (Department of Green Life Science, Sangmyung University) ;
  • Ki, Jang-Seu (Department of Green Life Science, Sangmyung University)
  • 천주용 (상명대학교 그린생명과학과) ;
  • 이민아 (상명대학교 그린생명과학과) ;
  • 기장서 (상명대학교 그린생명과학과)
  • Received : 2012.11.28
  • Accepted : 2012.12.14
  • Published : 2012.12.31

Abstract

In this study, we investigated molecular divergences and phylogenetic characteristics of the 16S ribosomal RNA (rRNA) and RNA polymerase beta subunit (rpoB) gene sequences from the order Oscillatoriales (Cyanobacteria). The rpoB of Oscillatoriales showed higher genetic divergence when compared with those of 16S rRNA (p-distance: rpoB=0.270, 16S=0.109), and these differences were statistically significant (Student t-test, p<0.001). Phylogenetic trees of 16S rRNA and rpoB were generally compatible; however, rpoB tree clearly separated the compared Oscillatoriales taxa, with higher phylogenetic resolution. In addition, parsimony analyses showed that rpoB gene evolved 2.40-fold faster than 16S rRNA. These results suggest that the rpoB is a useful gene for the molecular phylogenetics and species discrimination in the order Oscillatoriales.

Keywords

Oscillatoriales;rpoB gene;Cyanobacteria;DNA similarity;genetic distance

Acknowledgement

Supported by : 상명대학교

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