- Volume 48 Issue 4
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Virus Inactivation during the Manufacture of a Collagen Type I from Bovine Hides
소 가죽 유래 Type I Collagen 생산 공정에서 바이러스 불활화
- Bae, Jung Eun (Department of Biological Sciences and Biotechnology, Center for Biopharmaceuticals Safety Validation, Hannam University) ;
- Kim, Chan Kyung (Department of Biological Sciences and Biotechnology, Center for Biopharmaceuticals Safety Validation, Hannam University) ;
- Kim, Sungpo (R&D Center, Bioland Co. Ltd.) ;
- Yang, Eun Kyung (R&D Center, Bioland Co. Ltd.) ;
- Kim, In Seop (Department of Biological Sciences and Biotechnology, Center for Biopharmaceuticals Safety Validation, Hannam University)
- 배정은 (한남대학교 생명.나노과학대학 생명시스템과학과 & 바이오의약품안전성검증센터) ;
- 김찬경 (한남대학교 생명.나노과학대학 생명시스템과학과 & 바이오의약품안전성검증센터) ;
- 김성포 ((주)바이오랜드 조직공학연구소) ;
- 양은경 ((주)바이오랜드 조직공학연구소) ;
- 김인섭 (한남대학교 생명.나노과학대학 생명시스템과학과 & 바이오의약품안전성검증센터)
- Received : 2012.10.29
- Accepted : 2012.12.12
- Published : 2012.12.31
Most types of collagen used for biomedical applications, such as cell therapy and tissue engineering, are derived from animal tissues. Therefore, special precautions must be taken during the production of these proteins in order to assure against the possibility of the products transmitting infectious diseases to the recipients. The ability to remove and/or inactivate known and potential viral contaminants during the manufacturing process is an ever-increasingly important parameter in assessing the safety of biomedical products. The purpose of this study was to evaluate the efficacies of the 70% ethanol treatment and pepsin treatment at pH 2.0 for the inactivation of bovine viruses during the manufacture of collagen type I from bovine hides. A variety of experimental model viruses for bovine viruses including bovine herpes virus (BHV), bovine viral diarrhea virus (BVDV), bovine parainfluenza 3 virus (BPIV-3), and bovine parvovirus (BPV), were chosen for the evaluation of viral inactivation efficacy. BHV, BVDV, BPIV-3, and BPV were effectively inactivated to undetectable levels within 1 h of 70% ethanol treatment for 24 h, with log reduction factors of
bovine collagen;ethanol treatment;pepsin treatment;virus safety
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