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Characterization of a Chitinase Gene and Screening of Cold Active Chitinase from Polar Microorganisms

극지유래 저온활성 Chitinase 생산균주의 스크리닝과 Chitinase 유전자 클로닝

  • Park, Yu Kyung (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Kim, Jung Eun (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Lee, Hyoungseok (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Kim, Ji Hyun (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Park, Ha Ju (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Kim, Dockyu (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Park, Mira (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Yim, Joung Han (Division of Life Sciences, Korea Polar Research Institute (KOPRI)) ;
  • Kim, Il-Chan (Division of Life Sciences, Korea Polar Research Institute (KOPRI))
  • 박유경 (극지연구소 생명과학연구부) ;
  • 김정은 (극지연구소 생명과학연구부) ;
  • 이형석 (극지연구소 생명과학연구부) ;
  • 김지현 (극지연구소 생명과학연구부) ;
  • 박하주 (극지연구소 생명과학연구부) ;
  • 김덕규 (극지연구소 생명과학연구부) ;
  • 박미라 (극지연구소 생명과학연구부) ;
  • 임정한 (극지연구소 생명과학연구부) ;
  • 김일찬 (극지연구소 생명과학연구부)
  • Received : 2012.07.27
  • Accepted : 2012.11.05
  • Published : 2012.12.31

Abstract

Of the 169 strains of microorganisms stored in Polar and Alpine Microbial Collection of Korea Polar Research Institute, 27 strains were selected for their chitinase activity on ZoBell plates supplemented with 0.4% colloidal chitin. Among them, PAMC 21693 strain have shown the highest chitinolytic enzyme activity toward pNP-$(GlcNAc)_1$ at low temperature and the highest growth rate at $4^{\circ}C$. We cloned a full-length chitinase gene of 2,857 bp which contains an open reading frame of 2,169 bp encoding 872-amino acid polypeptide. Recombinant chitinase protein was expressed in E. coli and its molecular weight was confirmed 96 kDa. In this paper, we suggest the potential use of cold-active chitinase from polar microorganisms in the field of biotechnology.

Keywords

chitinase;cold-active chitinase;iPCR;polar microorganisms;screening

Acknowledgement

Supported by : 극지연구소

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