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Isolation and Characterization of Streptomyces spp. from Soil Showing Broad Spectrum Antibiotic Activity

광범위한 항균활성을 보이는 토양 유래 Streptomyces 속 방선균의 분리 및 특성 연구

  • Park, Sewook (Department of Microbiology and Molecular Biology, Chungnam National University) ;
  • Bae, Taeok (School of Medicine, Indiana University) ;
  • Kim, Seung Bum (Department of Microbiology and Molecular Biology, Chungnam National University)
  • Received : 2012.11.16
  • Accepted : 2012.12.12
  • Published : 2012.12.31

Abstract

Three actinobacterial strains exhibiting broad spectrum antibiotic activities were isolated from soil, and characterized. Through the comparative analysis of 16S rRNA genes, the three isolates could be assigned to the genus Streptomyces, as S. tanashiensis, S. nashivillensis, and S. rubiginosohelvolus were found to be the mostly related species, but the strains formed independent phylogenetic lineage. Each strain exhibited different antimicrobial profile against Gram-positive bacteria Bacillus subtilis and Staphylococcus aureus, Gram-negative bacteria Salmonella typhi, Enterobacter cloacae, Serratia marcescens, and Pseudomonas aeruginosa, and also fungi Candida tropicalis and Candida krusei. In addition to the antimicrobial profile, the strains also differed in API ZYM test results, which implies that the three strains might produce difference antimicrobial substances.

Keywords

Streptomyces;antimicrobial activity

Acknowledgement

Supported by : 한국연구재단

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