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Molecular Diversity of Rhizobacteria in Ginseng Soil and Their Plant Benefiting Attributes

인삼토양 내 근권세균의 다양성 및 식물에의 유용 특성

  • Hong, Eun Hye (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Lee, Sun Hee (Department of Microbiology & Molecular Biology, Chungnam National University) ;
  • Vendan, Regupathy Thamizh (Agricultural College & Research Institute, Tamil Nadu Agricultural University) ;
  • Rhee, Young Ha (Department of Microbiology & Molecular Biology, Chungnam National University)
  • 홍은혜 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 이선희 (충남대학교 생명시스템과학대학 미생물.분자생명과학과) ;
  • 알 타미즈 벤단 (인도 타밀나두 농업대학교) ;
  • 이영하 (충남대학교 생명시스템과학대학 미생물.분자생명과학과)
  • Received : 2012.10.02
  • Accepted : 2012.12.14
  • Published : 2012.12.31

Abstract

The purpose of this study was to investigate the molecular diversity of rhizobacteria associated with ginseng of varying age levels and their plant benefiting attributes. A total of 143 different isolates belonging to 15 different bacterial genera were recovered. Although variation was found in the rhizobacterial community due to age of the plant, majority of bacteria belong to Firmicutes (58%). In which, Bacillus was found to be the predominant genus irrespective of age of the ginseng. To assess the plant benefiting attributes, 30 representative isolates were selected. The results indicated that some of the isolates could exhibit multiple plant growth promoting traits like secretion of cell wall degrading enzymes, production of indole-3-acetic acid, synthesis of siderophores, solubilization of phosphates and soil pathogens inhibition. It can be suggested that strains of B. subtilis, B. amyloliquefaciens, B. velezensis, and B. licheniformis were positive for all the above traits, which have potential to be used as plant growth promoting inoculants to improve ginseng crop in the future.

Keywords

diversity;ginseng;plant growth promoting rhizobacteria (PGPR);rhizobacteria

References

  1. Bai, Y., Frederic, D.A., Donald, L.S., and Brian, T.D. 2002. Isolation of plant-growth-promoting Bacillus strains from soybean root nodules. Can. J. Microbiol. 48, 230-238. https://doi.org/10.1139/w02-014
  2. Bashan, Y., Holguin, G., and de-Bashan, L.E. 2004. Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances. Can. J. Microbiol. 50, 521-577. https://doi.org/10.1139/w04-035
  3. Banchio, E., Bogino, P.C., Zygadlo, J., and Giordano, W. 2008. Plant growth promoting rhizobacteria improve growth and essential oil yield in Origanum majorana L. Biochem. Syst. Ecol. 36, 766-771. https://doi.org/10.1016/j.bse.2008.08.006
  4. Calvo, P., Orrillo, E.O., Romero, E.M., and Zuniga, D. 2010. Characterization of Bacillus isolates of potato rhizosphere from Andean soils of Peru and their potential PGPR characteristics. Braz. J. Microbiol. 41, 899-906. https://doi.org/10.1590/S1517-83822010000400008
  5. Chaiharn, M. and Lumyong, S. 2009. Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Northern Thailand. World J. Microbiol. Biotechnol. 25, 305-314. https://doi.org/10.1007/s11274-008-9892-2
  6. Chang, W.T., Chen, Y.C., and Jao, C.L. 2007. Antifungal activity and enhancement of plant growth by Bacillus cereus grown on shellfish chitin wastes. Bioresour. Technol. 98, 1224-1230. https://doi.org/10.1016/j.biortech.2006.05.005
  7. Chatli, A.S., Beri, V., and Sidhu, B.S. 2008. Isolation and characterisation of phosphate solubilising microorganisms from the cold desert habitat of Salix alba Linn. in trans Himalayan region of Himachal Pradesh. Indian J. Microbiol. 48, 267-273. https://doi.org/10.1007/s12088-008-0037-y
  8. Goldstein, A.H. 1986. Bacterial solubilization of mineral phosphates: historical perspective and future prospects. Am. J. Alter. Agric. 1, 51-57. https://doi.org/10.1017/S0889189300000886
  9. Han, J., Xia, D., Li, L., Sun, L., Yang, K., and Zhang, L. 2009. Diversity of culturable bacteria isolated from root domains of Moso Bamboo (Phyllostachys edulis). Microb. Ecol. 58, 363-373. https://doi.org/10.1007/s00248-009-9491-2
  10. Hartmann, A., Singh, M., and Klingmueller, W. 1983. Isolation and characterization of Azospirillum mutants excreting high amounts of indole acetic acid. Can. J. Microbiol. 29, 916-923. https://doi.org/10.1139/m83-147
  11. Hynes, R.K., Leung, G.C., Hirkala, D.L., and Nelson, L.M. 2008. Isolation, selection, and characterization of beneficial rhizobacteria from pea, lentil, and chickpea grown in western Canada. Can. J. Microbiol. 54, 248-258. https://doi.org/10.1139/W08-008
  12. Joshi, P. and Bhatt, A.B. 2011. Diversity and function of plant growth promoting rhizobacteria associated with wheat rhizosphere in North Himalayan region. Int. J. Environ. Sci. 1, 1135-1143.
  13. Joshi, P., Tyagi, V., and Bhatt, A.B. 2011. Characterization of rhizobacteria diversity isolated from Oryza sativa cultivated at different altitude in North Himalaya. Adv. Appl. Sci. Res. 4, 208-216.
  14. Lee, C.S., Kim, K.D., Hyun, J.W., and Jeun, Y.C. 2003. Isolation of rhizobacteria in Jeju island showing anti-fungal effect against fungal plant pathogens. Mycobiol. 31, 251-254. https://doi.org/10.4489/MYCO.2003.31.4.251
  15. Lee, S., Ka, J.-O., and Song, H.-G. 2012. Growth promotion of Xanthium italicum by application of rhizobacterial isolates of Bacillus aryabhattai in microcosm soil. J. Microbiol. 50, 45-49. https://doi.org/10.1007/s12275-012-1415-z
  16. Lugtenberg, B. and Kamilova, F. 2009. Plant-growth-promoting rhizobacteria. Annu. Rev. Microbiol. 63, 541-556. https://doi.org/10.1146/annurev.micro.62.081307.162918
  17. McGinnis, S. and Madden, T.L. 2004. BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Res. 32, 20-25.
  18. Nautiyal, C.S. 1999. An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett. 170, 265-270. https://doi.org/10.1111/j.1574-6968.1999.tb13383.x
  19. Noura, R., Ameur, C., Abdellatif, B., and Daniele, D. 2008. Screening of plant growth promoting traits of Bacillus thuringiensis. Ann. Microbiol. 58, 47-52. https://doi.org/10.1007/BF03179444
  20. Pereira, P., Ibanez, F., Rosenblueth, M., Etcheverry, M., and Martinez- Romero, E. 2011. Analysis of the bacterial diversity associated with the roots of Maize (Zea mays L.) through culture-dependent and culture-independent methods. ISRN Ecology, Volume 2011, Article ID 938546, 10 pages, doi:10.5402/2011/938546. https://doi.org/10.5402/2011/938546
  21. Ramos, B., Pozuelo, J.M., Acero, N., and Gutierrez Manero, F.J. 1998. Seasonal variation of Bacillus isolates from the rhizosphere of Elaeagnus angustifolia L. Orsis. 13, 7-16.
  22. Rroco, E., Kosegarten, H., Harizaj, F., Imani, J., and Mengel, K. 2003. The importance of soil microbial activity for the supply of iron to sorghum and rape. Europ. J. Agron. 19, 487-493. https://doi.org/10.1016/S1161-0301(02)00185-5
  23. Sadfi, N., Cherif, M., Hajlaoui, M.R., Boudabbous, A., and Belanger, R. 2002. Isolation and partial purification of antifungal metabolites produced by Bacillus cereus. Ann. Microbiol. 52, 323-337.
  24. Schwyn, B. and Neilands, J.B. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160, 47 -56. https://doi.org/10.1016/0003-2697(87)90612-9
  25. Selvadurai, E.L., Brown, A.E., and Hamilton, J.T.G. 1991. Production of indole-3-acetic acid analogues by strains of Bacillus cereus in relation to their influence on seedling development. Soil Biol. Biochem. 23, 401-403. https://doi.org/10.1016/0038-0717(91)90198-S
  26. Sharma, A., Johri, B.N., Sharma, A.K., and Glick, B.R. 2003. Plant growth promoting bacterium Pseudomonas sp., strain GRP3 influences iron acquisition in mung bean (Vigna radiate L. Wilzeck). Soil Biol. Biochem. 35, 887-894. https://doi.org/10.1016/S0038-0717(03)00119-6
  27. Tamura, K., Dudley, J., Nei, M., and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596-1599. https://doi.org/10.1093/molbev/msm092
  28. Tompson, J.D., Higgins, D.G., and Gibson, T.J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22, 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  29. Vendan, R.T., Yu, Y.J., Lee, S.H., and Rhee, Y.H. 2010. Diversity of endophytic bacteria in ginseng and their potential for plant growth promotion. J. Microbiol. 48, 559-565. https://doi.org/10.1007/s12275-010-0082-1
  30. Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571-586. https://doi.org/10.1023/A:1026037216893
  31. Yang, J., Kloepper, J.W., and Ryu, C.M. 2009. Rhizosphere bacteria help plants tolerate abiotic stress. Trends Plant Sci. 14, 1-4. https://doi.org/10.1016/j.tplants.2008.10.004
  32. Yu, W.J., Lee, B.J., Nam, S.Y., Yang, D.C., and Yun, Y.W. 2003. Modulating effects of Korean ginseng saponins on ovarian function immature rats. Biol. Pharm. Bull. 26, 2574-2580.

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