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The Chloroform Fraction of Carpinus tschonoskii Leaves Inhibits the Production of Inflammatory Mediators in HaCaT Keratinocytes and RAW264.7 Macrophages

  • Kang, Gyeoung-Jin ;
  • Kang, Na-Jin ;
  • Han, Sang-Chul ;
  • Koo, Dong-Hwan ;
  • Kang, Hee-Kyoung ;
  • Yoo, Byoung-Sam ;
  • Yoo, Eun-Sook
  • Received : 2012.09.18
  • Accepted : 2012.12.14
  • Published : 2012.12.31

Abstract

Inflammation is the immune system's response to infection and injury-related disorders, and is related to pro-inflammatory factors (NO, $PGE_2$, cytokines, etc.) produced by inflammatory cells. Atopic dermatitis (AD) is a representative inflammatory skin disease that is characterized by increasing serum levels of inflammatory chemokines, including macrophage-derived chemokine (MDC). Carpinus tschonoskii is a member of the genus Carpinus. We investigated the anti-inflammatory activity of C. tschonoskii by studying the effects of various solvent fractions prepared from its leaves on inflammatory mediators in HaCaT and RAW264.7 cells. We found that the chloroform fraction of C. tschonoskii inhibited MDC at both the protein and mRNA levels in HaCaT cells, acting via the inhibition of STAT1 in the IFN-${\gamma}$ signaling pathway. In addition, the chloroform fraction significantly suppressed the expression of inflammatory factors induced by lipopolysaccharide stimulation, except COX-2 and TNF-${\alpha}$. These results suggest that the chloroform fraction of C. tschonoskii leaves may include a component with potential anti-inflammatory activity.

Keywords

Carpinus tschonoskii;Inflammation mediators;HaCaT keratinocytes;RAW264.7 macrophages

References

  1. Coleman, J.W. (2001). Nitric oxide in immunity and inflammation. Int. Immunopharmacol., 1, 1397-1406. https://doi.org/10.1016/S1567-5769(01)00086-8
  2. Damte, D., Reza, M.A., Lee, S.J., Jo, W.S. and Park, S.C. (2011). Anti-inflammatory activity of dichloromethane extract of auricularia auricula-judae in RAW264.7 cells. Toxicol. Res., 27, 11-14. https://doi.org/10.5487/TR.2011.27.1.011
  3. Duffield, J.S. (2003). The inflammatory macrophage: a story of Jekyll and Hyde. Clin. Sci. (Lond), 104, 27-38. https://doi.org/10.1042/CS20020240
  4. Gough, D.J., Levy, D.E., Johnstone, R.W. and Clarke, C.J. (2008). IFNgamma signaling-does it mean JAK-STAT? Cytokine Growth Factor Rev., 19, 383-394. https://doi.org/10.1016/j.cytogfr.2008.08.004
  5. Hammer, K.D., Yum, M.Y., Dixon, P.M. and Birt, D.F. (2010). Identification of JAK-STAT pathways as important for the antiinflammatory activity of a Hypericum perforatum fraction and bioactive constituents in RAW 264.7 mouse macrophages. Phytochemistry, 71, 716-725. https://doi.org/10.1016/j.phytochem.2010.02.006
  6. Hongqin, T., Xinyu, L., Heng, G., Lanfang, X., Yongfang, W. and Shasha, S. (2011). Triptolide inhibits IFN-gamma signaling via the Jak/STAT pathway in HaCaT keratinocytes. Phytother. Res., 25, 1678-1685. https://doi.org/10.1002/ptr.3471
  7. Hu, X., Chen, J., Wang, L. and Ivashkiv, L.B. (2007). Crosstalk among Jak-STAT, Toll-like receptor, and ITAM-dependent pathways in macrophage activation. J. Leukocyte Biol., 82, 237-243. https://doi.org/10.1189/jlb.1206763
  8. Jeon, J.I. and Chang, C.S. (2000). Foliar flavonoids of genus carpinus in eastern Asia. Kor. J. Plant Tax., 30, 139-153.
  9. Jeong, S.I., Choi, B.M. and Jang, S.I. (2010). Sulforaphane suppresses TARC/CCL17 and MDC/CCL22 expression through heme oxygenase-1 and NF-kappaB in human keratinocytes. Arch. Pharm. Res., 33, 1867-1876. https://doi.org/10.1007/s12272-010-1120-6
  10. Ju, S.M., Song, H.Y., Lee, S.J., Seo, W.Y., Sin, D.H., Goh, A.R., Kang, Y.H., Kang, I.J., Won, M.H., Yi, J.S., Kwon, D.J., Bae, Y.S., Choi, S.Y. and Park, J. (2009). Suppression of thymusand activation-regulated chemokine (TARC/CCL17) production by 1,2,3,4,6-penta-O-galloyl-beta-D-glucose via blockade of NF-kappaB and STAT1 activation in the HaCaT cells. Biochem. Biophys. Res. Commun., 387, 115-120. https://doi.org/10.1016/j.bbrc.2009.06.137
  11. Kakinuma, T., Nakamura, K., Wakugawa, M., Mitsui, H., Tada, Y., Saeki, H., Torii, H., Komine, M., Asahina, A. and Tamaki, K. (2002). Serum macrophage-derived chemokine (MDC) levels are closely related with the disease activity of atopic dermatitis. Clin. Exp. Immunol., 127, 270-273. https://doi.org/10.1046/j.1365-2249.2002.01727.x
  12. Kang, G.J., Han, S.C., Yi, E.J., Kang, H.K. and Yoo, E.S. (2011). The inhibitory effect of premature Citrus unshiu extract on atopic dermatitis in vitro and in vivo. Toxicol. Res., 27, 173-180. https://doi.org/10.5487/TR.2011.27.3.173
  13. Kaplan, A.P. (2001). Chemokines, chemokine receptors and allergy. Int. Arch. Allergy Immunol., 124, 423-431. https://doi.org/10.1159/000053777
  14. Koo, J.E., Hong, H.J., Mathema, V.B., Kang, H.K., Hyun, J.W., Kim, G.Y., Kim, Y.R., Maeng, Y.H., Hyun, C.L., Chang, W.Y. and Koh, Y.S. (2012). Inhibitory effects of Carpinus tschonoskii leaves extract on CpG-stimulated pro-inflammatory cytokine production in murine bone marrow-derived macrophages and dendritic cells. In Vitro Cell. Dev. Biol. Anim., 48, 197-202. https://doi.org/10.1007/s11626-012-9495-y
  15. Lee, H.J., Dang, H.T., Kang, G.J., Yang, E.J., Park, S.S., Yoon, W.J., Jung, J.H., Kang, H.K. and Yoo, E.S. (2009). Two enone fatty acids isolated from Gracilaria verrucosa suppress the production of inflammatory mediators by down-regulating NFkappaB and STAT1 activity in lipopolysaccharide-stimulated RAW 264.7 cells. Arch. Pharm. Res., 32, 453-462. https://doi.org/10.1007/s12272-009-1320-0
  16. Leung, T.F., Ma, K.C., Hon, K.L., Lam, C.W., Wan, H., Li, C.Y. and Chan, I.H. (2003). Serum concentration of macrophagederived chemokine may be a useful inflammatory marker for assessing severity of atopic dermatitis in infants and young children. Pediatr. Allergy Immunol., 14, 296-301. https://doi.org/10.1034/j.1399-3038.2003.00052.x
  17. Li, C.C., Hsiang, C.Y., Lo, H.Y., Pai, F.T., Wu, S.L. and Ho, T.Y. (2012). Genipin inhibits lipopolysaccharide-induced acute systemic inflammation in mice as evidenced by nuclear factor-kappaB bioluminescent imaging-guided transcriptomic analysis. Food Chem. Toxicol., 50, 2978-2986. https://doi.org/10.1016/j.fct.2012.05.054
  18. Luster, A.D. (2001). Antichemokine immunotherapy for allergic diseases. Curr. Opin. Allergy Clin. Immunol., 1, 561-567. https://doi.org/10.1097/00130832-200112000-00012
  19. MacMicking, J.D., Nathan, C., Hom, G., Chartrain, N., Fletcher, D.S., Trumbauer, M., Stevens, K., Xie, Q.W., Sokol, K. and Hutchinson, N. (1995). Altered responses to bacterial infection and endotoxic shock in mice lacking inducible nitric oxide synthase. Cell., 81, 641-650. https://doi.org/10.1016/0092-8674(95)90085-3
  20. Mantovani, A., Gray, P.A., Van Damme, J. and Sozzani, S. (2000). Macrophage-derived chemokine (MDC). J. Leukocyte Biol., 68, 400-404.
  21. Marcuzzi, A., Secchiero, P., Crovella, S. and Zauli, G. (2012). TRAIL administration down-modulated the acute systemic inflammatory response induced in a mouse model by muramyldipeptide or lipopolysaccharide. Cytokine, 60, 43-46. https://doi.org/10.1016/j.cyto.2012.06.001
  22. Maruotti, N., Cantatore, F.P., Crivellato, E., Vacca, A. and Ribatti, D. (2007). Macrophages in rheumatoid arthritis. Histol. Histopathol., 22, 581-586.
  23. Michelsen, K.S., Doherty, T.M., Shah, P.K. and Arditi, M. (2004). TLR signaling: an emerging bridge from innate immunity to atherogenesis. J. Immunol., 173, 5901-5907. https://doi.org/10.4049/jimmunol.173.10.5901
  24. Palsson-McDermott, E.M. and O'Neill, L.A. (2004). Signal transduction by the lipopolysaccharide receptor, Toll-like receptor-4. Immunol., 113, 153-162. https://doi.org/10.1111/j.1365-2567.2004.01976.x
  25. Pease, J.E. and Williams, T.J. (2006). Chemokines and their receptors in allergic disease. J. Allergy Clin. Immunol., 118, 305-318. https://doi.org/10.1016/j.jaci.2006.06.010
  26. Ricciotti, E. and FitzGerald, G.A. (2011). Prostaglandins and inflammation. Arterioscler. Thromb. Vasc. Biol., 31, 986-1000. https://doi.org/10.1161/ATVBAHA.110.207449
  27. Stempelj, M., Kedinger, M., Augenlicht, L. and Klampfer, L. (2007). Essential role of the JAK/STAT1 signaling pathway in the expression of inducible nitric-oxide synthase in intestinal epithelial cells and its regulation by butyrate. J. Biol. Chem., 282, 9797-9804. https://doi.org/10.1074/jbc.M609426200
  28. Takeshita, F., Gursel, I., Ishii, K.J., Suzuki, K., Gursel, M. and Klinman, D.M. (2004). Signal transduction pathways mediated by the interaction of CpG DNA with Toll-like receptor 9. Semin. Immunol., 16, 17-22. https://doi.org/10.1016/j.smim.2003.10.009
  29. Ulevitch, R.J., Mathison, J.C. and da Silva Correia, J. (2004). Innate immune responses during infection. Vaccine, 22 Suppl 1, S25-S30. https://doi.org/10.1016/j.vaccine.2004.08.013
  30. van Boxel-Dezaire, A.H. and Stark, G.R. (2007). Cell type-specific signaling in response to interferon-gamma. Curr. Top. Microbiol. Immunol., 316, 119-154. https://doi.org/10.1007/978-3-540-71329-6_7
  31. Yamada, P., Ono, T., Shigemori, H., Han, J. and Isoda, H. (2012). Inhibitory effect of tannins from galls of Carpinus tschonoskii on the degranulation of RBL-2H3 Cells. Cytotechnology, 64, 349-356. https://doi.org/10.1007/s10616-012-9457-y
  32. Zhang, R., Kang, K.A., Piao, M.J., Park, J.W., Shin, T., Yoo, B.S., Yang, Y.T. and Hyun, J.W. (2007). Cytoprotective activity of Carpinus tschonoskii against $H_{2}O_{2}$ induced oxidative stress. Nat. Prod. Sci., 13, 118-122.

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