Visualization of Artificially Deposited Submicron-sized Aerosol Particles on the Surfaces of Leaves and Needles in Trees

  • Yamane, Kenichi ;
  • Nakaba, Satoshi ;
  • Yamaguchi, Masahiro ;
  • Kuroda, Katsushi ;
  • Sano, Yuzou ;
  • Lenggoro, I. Wuled ;
  • Izuta, Takeshi ;
  • Funada, Ryo
  • Received : 2012.06.22
  • Accepted : 2012.10.18
  • Published : 2012.12.31


To understand the effect of aerosols on the growth and physiological conditions of trees in forests, it is important to know the state of aerosols that are deposited on the surface of the leaves or needles. In this study, we developed methods of visualization of submicron-sized aerosols that were artificially deposited from the gas-phase or liquid phase onto tree leaves or needles in trees. Firstly, we used field-emission scanning electron microscopy (FE-SEM) to observe black carbon (BC) particles that were artificially sprayed onto the leaves or needles. The distribution of BC particles deposited on the leaves and needles were distinguished based on the size and morphological features of the particles. The distribution and agglomerates size of BC particles differed between two spraying methods of BC particles employed. Secondly, we tried to visualize gold (Au) particles that were artificially sprayed onto the leaves using energy dispersive X-ray spectrometry (EDX) coupled to FE-SEM. We detected the Au particles based on the characteristic X-ray spectrum, which was secondarily generated from the Au particles. In contrast to the case of BC particles, the Au particles did not form agglomerates and were uniformly distributed on the leaf surfaces. The present results show that our methods provide useful information of adsorption and/or behavior of fine particles at the submicron level on the surface of the leaves.


Forest tree;Black carbon (BC) particle;Gold (Au) particle;Field-emission scanning electron microscopy (FE-SEM);Energy dispersive X-ray spectrometry (EDX)


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Cited by

  1. Canopy, leaf surface structure and tree phenology: Arboreal factors influencing aerosol deposition in forests vol.71, pp.3, 2015,
  2. Effect of epicuticular wax crystals on the localization of artificially deposited sub-micron carbon-based aerosols on needles of Cryptomeria japonica vol.129, pp.5, 2016,


Supported by : Japan Society for the Promotion of Science