Oleuropein Induces Anti-metastatic Effects in Breast Cancer

  • Hassan, Zeinab K. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Elamin, Maha H. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Daghestani, Maha H. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Omer, Sawsan A. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Al-Olayan, Ebtesam M. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Elobeid, Mai A. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Virk, Promy (Department of Zoology, College of Science, King Saud University, University Centre for Women Students) ;
  • Mohammed, Osama B. (Department of Zoology, College of Science, King Saud University, University Centre for Women Students)
  • Published : 2012.09.30


Breast cancer causes death due to distant metastases in which tumor cells produce matrix metalloproteinase (MMP) enzymes which facilitate invasion. Oleuropein, the main olive oil polyphenol, has anti-proliferative effects. This study aimed to investigate the effect of oleuropein on the metastatic and anti-metastatic gene expression in the MDA human breast cancer cell line. We evaluated the MMPs and TIMPs gene expression by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) in treated and untreated cells. This study demonstrated that OL may induce anti-metastatic effects on human breast cancer cells. We found that TIMP1,-3, and -4 were over-expressed after all periods of incubation in treated cancer cells compared to untreated cells, while MMP2 and MMP9 genes were down-regulated, at least initially. Treatment of breast cancer cells with oleuropein could help in prevention of cancer metastasis by increasing the TIMPs and suppressing the MMPs gene expressions.


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