- Volume 13 Issue 9
DOI QR Code
Up-regulation of NICE-3 as a Novel EDC Gene Could Contribute to Human Hepatocellular Carcinoma
- Wei, Yuan-Jiang (Department of Hepatobiliary Surgery, Wuxi Municipal People's Hospital of Nanjing Medical University) ;
- Hu, Qin-Qin (Deaprtment of Pain Treatment, The Affiliated Hospital of Jiujiang University) ;
- Gu, Cheng-Yu (Department of Hepatobiliary Surgery, Wuxi Municipal People's Hospital of Nanjing Medical University) ;
- Wang, Yu-Ping (Chinese National Human Genome Center, Rui-Jin Hospital, Shanghai Jiaotong University School of Medicine) ;
- Han, Ze-Guang (Chinese National Human Genome Center, Rui-Jin Hospital, Shanghai Jiaotong University School of Medicine) ;
- Cai, Bing (Department of Hepatobiliary Surgery, Wuxi Municipal People's Hospital of Nanjing Medical University)
- Published : 2012.09.30
The epidermal differentiation complex (EDC) contains a large number of gene products which are crucial for the maturation of the human epidermis and can contribute to skin diseases, even carcinogenesis. It is generally accepted that activation of oncogenes and/or inactivation of tumor suppressor genes play pivotal roles in the process of carcinogenesis. Here, NICE-3, a novel EDC gene, was found to be up-regulated in human hepatocellular carcinoma (HCC) by quantitative real-time RT-PCR. Furthermore, overexpression of exogenous NICE-3 by recombinant plasmids could significantly promote cell proliferation, colony formation and soft agar colony formation in Focus and WRL-68 HCC cell lines. Reversely, NICE-3 silencing by RNA interference could markedly inhibit these malignant phenotypes in YY-8103 and MHCC-97H cells. Moreover, cell cycle analysis of MHCC-97H transfected with siRNA by flow cytometry showed that NICE-3 knockdown may inhibit cell growth via arrest in G0/G1 phase and hindering entry of cells into S phase. All data of our findings indicate that NICE-3 may contribute to human hepatocellular carcinoma by promoting cell proliferation.
Andrew M, Jamel M, Mark H (2010). Hepatitis C virus NS5A protein interacts with
$\beta$-catenin and stimulates its transcriptional activity in a phosphoinositide-3 kinasedependent fashion. Journal of General Virology, 91, 373-81. https://doi.org/10.1099/vir.0.015305-0
- Chung YL, Sheu ML, Yen SH (2003). Hepatitis C Virus NS5A as a potential viral Bcl-2 homologue interacts with Bax and inhibits apoptosis in hepatocellular carcinoma. Int J Cancer, 107, 65-73. https://doi.org/10.1002/ijc.11303
- Deng Q, Wang Q, Zong WY, et al (2010). E2F8 Contributes to Human Hepatocellular Carcinoma via Regulating Cell Proliferation. Cancer Res, 70, 782-91. https://doi.org/10.1158/0008-5472.CAN-09-3082
- Elin K, et al (2008). Gene expression variation to predict 10- year survival in lymph-node-negative breast cancer. BMC Cancer, 8, 254-67 https://doi.org/10.1186/1471-2407-8-254
- Gimenez-Barcons M, Wang C, Chen M, et al (2005). The oncogenic potential of hepatitis C virus NS5A sequence variants is associated with PKR regulation. J Interferon Cytokine Res, 2005,152-64.
- Huang J, Lin Y, Li L, Qing D. et al (2009). ARHI, as a novel suppressor of cell growth and downregulated in human hepatocellular carcinoma, could contribute to hepatocarcinogenesis. Mol Carcinog, 48, 130-40. https://doi.org/10.1002/mc.20461
- Huang J, Zhang X, Zhang M, et al (2007). Up-regulation of DLKl as an imprinted gene could contribute to hurnan hepatocellular carcinoma. Carcinogenesis, 28,1094-03.
- Huang J, Zhang YL, Teng XM, et al (2007). Down-regulation of SFRPl as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma. BMC Cancer, 7, 126-40. https://doi.org/10.1186/1471-2407-7-126
- Huang J, Zheng DL, Qin FS, et al (2010). Genetic and epigenetic silencing of SCARA5 may contribute to human hepatocellular carcinoma by activating FAK signaling. J Clin Invest, 120, 223-41. https://doi.org/10.1172/JCI38012
- Ingo M, Maja Z, David F, et al (2001). Identification of Human Epidermal Differentiation Complex (EDC)-Encoded Genes by Subtractive Hybridization of Entire YACs to a Gridded Keratinocyte cDNA Library. Genome Res, 11,341-55. https://doi.org/10.1101/gr.114801
- Kato N, Lan KH, Ono-Nita SK, et al (1997). Hepatitis C virus nonstructural region 5A protein is a potent transcriptional activator. J Virol, 71, 8856-9.
- Keng-Hsin L, Meei-Ling S, Shinn-Jang H, et al (2002). HCV NS5A interacts with p53 and inhibits p53-mediated apoptosis. Oncogene, 21, 4801-11. https://doi.org/10.1038/sj.onc.1205589
- Leung TH, Wong N, Lai PB, et al (2002). Identification of four distinct regions of allelic imbalances on chromosome 1 by the combined comparative genomic hybridization and microsatellite analysis on hepatocellular carcinoma. Mod Pathol, 15, 1213-20. https://doi.org/10.1097/01.MP.0000036347.66943.3C
- Maelandsmo GM, Florenes VA, Mellingsaeter T, et al (1997) Differential expression patterns of S100A2, S100A4 and S100A6 during progression of human malignant melanoma . Int. J Cancer, 74, 464-9. https://doi.org/10.1002/(SICI)1097-0215(19970822)74:4<464::AID-IJC19>3.0.CO;2-9
- Marenholz I, et al (2011). Association screening in the Epidermal Differentiation Complex (EDC) identifies an SPRR3 repeat number variant as a risk factor for eczema. J Invest Dermatol, 131,1644-9. https://doi.org/10.1038/jid.2011.90
- Martin CA, et al (2004). Transcriptomic changes inhuman breast cancer progression as determined by serial analysis of gene expression. Breast Cancer Res, 6, 499-13. https://doi.org/10.1186/bcr899
- Moubayed N, et al (2007). Psoriasin (S100A7) is significantly up-regulated in human epithelial skin tumours. J Cancer Res Clin Oncol, 133, 253-61. https://doi.org/10.1007/s00432-006-0164-y
- Nasser MW, et al (2012). S100A7 enhances mammary tumorigenesis through upregulation of inflammatory pathways. Cancer Res, 72, 604-15. https://doi.org/10.1158/0008-5472.CAN-11-0669
- Perz JF, Armstrong GL, Farrington LA, et al (2006). The contributions of hepatitis B virus and hepatitis C virus infections to cirrhosis and primary liver cancer worldwide. J Hepatol, 45, 529-38 https://doi.org/10.1016/j.jhep.2006.05.013
- Rajesh K, et al (2007). Fibrolamellar carcinomas show overexpression of genes in the RAS, MAPK, PHG, and xenobiotic degradation pathways. Human Pathology, 38, 639-44. https://doi.org/10.1016/j.humpath.2006.07.019
- Ruisheng Y, et al (2007). Expression of S100 protein family members in the pathogenesis of bladder tumors. Anticancer Res, 27, 3051-8
- Tripathi SC, Matta A, Kaur J, et al (2010). Nuclear S100A7 is associated with poor prognosis in head and neck cancer. PLoS ONE,5, e11939. https://doi.org/10.1371/journal.pone.0011939
- Wang HX, Li YD, Wang YP, et al (2012). C9orf100, a new member of the Dbl-family guanine nucleotide exchange factors, promotes cell proliferation and migration in hepatocellular carcinoma. Mol Med Report, 5, 1169-74
- Yang Q, Liu Y, Cheng J, et al (2003). Cloning and identification of gene 4 transactivated by non-structural protein 5A of hepatitis C virus. Chin J Gastro Hepa, 12, 248-50.
- Zhang QH, Ye M, Wu XY, et al (2000). Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res, 10, 1546-60. https://doi.org/10.1101/gr.140200
- Targeting SHCBP1 Inhibits Cell Proliferation in Human Hepatocellular Carcinoma Cells vol.14, pp.10, 2013, https://doi.org/10.7314/APJCP.2013.14.10.5645