Serum Oncofetal Fibronectin (onfFN) mRNA in Differentiated Thyroid Carcinoma (DTC): Large Overlap between Disease-Free and Metastatic Patients

  • Sritara, Chanika (Department of Diagnostic and Therapeutic Radiology, Faculty of Medicine Ramathibodi Hospital) ;
  • Charoenphun, Putthiporn (Department of Diagnostic and Therapeutic Radiology, Faculty of Medicine Ramathibodi Hospital) ;
  • Ponglikitmongkol, Mathurose (Department of Biochemistry, Faculty of Science, Mahidol University) ;
  • Musikarat, Suchawadee (Department of Diagnostic and Therapeutic Radiology, Faculty of Medicine Ramathibodi Hospital) ;
  • Utamakul, Chirawat (Department of Diagnostic and Therapeutic Radiology, Faculty of Medicine Ramathibodi Hospital) ;
  • Chokesuwattanasakul, Payap (Department of Diagnostic and Therapeutic Radiology, Faculty of Medicine Ramathibodi Hospital) ;
  • Thakkinstian, Ammarin (Section for Clinical Epidemiology and Biostatistics, Faculty of Medicine Ramathibodi Hospital)
  • Published : 2012.08.31


Aim: This study assessed if onfFN mRNA in the peripheral blood of patients with DTC can identify individuals with metastatic disease. Methods: Comparison of onfFN mRNA was made among 3 groups: disease-free, lymph node metastasis, and distant metastasis using real-time RT-PCR on 5 ml blood samples from each DTC patient. Results: Fifty-one patients were included: 30 (59%) were disease-free; 7 (13.7%) had lymph node metastasis; and 14 (27.5%) had distant metastasis. OnfFN mRNA levels in the 3 groups were significantly different (P=0.001) but with a large overlap and the expression being highest in the disease-free group. Subgroup analysis of the metastatic groups did not show any effect of age, cell type, and serum TSH, Tg, and antiTg on onfFN mRNA. The within-run and between-run root mean square coefficients of variations were <2%. Conclusion: OnfFN mRNA in patients with DTC cannot identify those with metastatic disease.


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