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Green Tea Polyphenol Protection Against 4-Nitroquinoline 1-Oxide-Induced Bone Marrow Lipid Peroxidation and Genotoxicity in Wistar Rats

  • Published : 2012.08.31

Abstract

4-Nitroquinoline 1-oxide (4-NQO) a potent oral carcinogen, widely used for induction of oral carcinogenesis, has been found to induce lipid peroxidation in vivo and in vitro. Green tea contains a high content of polyphenols, which are potent antioxidants. Thus green tea polyphenols (GTP) might be expected play a protective role against 4-NQO induced lipid peroxidation and bone marrow toxicity. In the present study, a dose of 200 mg of GTP/kg b.wt/day was given orally for a week, simultaneously animals received 0.2 ml of 0.5% 4-NQO in propylene glycol (5 mg/ml) injected intramuscularly for three times/week. Oxidants and antioxidants such as malendialdehyde (MDA) and thiols, glutathione peroxidase (GPx), glutathione reductase (GR), superoxide dismutase (SOD) and catalase (CAT) were significantly decreased in 4-NQO induced animals except MDA, and these parameters were brought back to near normalcy on treatment with GTP. The results suggest that GTP treatment offers significant protection against 4-NQO induced lipid peroxidation and bone marrow toxicity and might be a promising potential candidate for prevention of mutations leading to cancer.

References

  1. Aceto A, Di Ilio C, Lo BM, et al (1990). Differential activity of human, rat, mouse and bacteria glutathione transferase isoenzymes towards 4-nitroquinoline 1-oxide. Carcinogenesis, 11, 2267-9. https://doi.org/10.1093/carcin/11.12.2267
  2. Anderson RF, Amarasinghe C, Fisher LJ, et al (2000). Reduction in free-radical-induced DNA strand breaks and base damage through fast chemical repair by flavonoids. Free Radic Res, 33, 91-103. https://doi.org/10.1080/10715760000300651
  3. Bailleul B, Daubersies P, Galiegue-Zouitina S, Loucheux- Lefebvre MH (1989). Molecular basis of 4-nitroquinoline 1-oxide carcinogenesis. Jpn J Cancer Res, 80, 691-7. https://doi.org/10.1111/j.1349-7006.1989.tb01698.x
  4. Bellomo G, Mirabelli F, Richelmi P, Orrenius S (1983). Critical role of sulphydryl groups in ATP- dependent $Ca^{2+}$ sequestration by the plasma membrane fraction from rat liver. FEBS Lett, 163, 136-9. https://doi.org/10.1016/0014-5793(83)81180-6
  5. Benson AM (1993). Conversion of 4-nitroquinoline 1-oxide (4NQO) to 4-hydroxyaminoquinoline 1-oxide by a dicumarol resistant hepatic 4NQO nitroreductase in rats and mice. Biochem Pharmacol, 46, 1217-21. https://doi.org/10.1016/0006-2952(93)90470-H
  6. Booth DR (1990). A relationship found between intra-oral sites of 4NQO reductase activity and chemical carcinogenesis. Cell Tissue Kinet, 23, 331-40.
  7. Casini AF, Maellaro E, Pompella A, et al (1987). Lipid peroxidation, protein thiols and calcium homeostasis in bromobenzene induced liver damage. Biochem Pharmacol, 36, 3689. https://doi.org/10.1016/0006-2952(87)90021-9
  8. Cederbaum AI, Cohen G (1984). In: Methods in enzymology. Packer L. editor Academic press, San Diego, 105, 516-22.
  9. Cerutti PA (1994). Oxyradicals and cancer. Lancet, 344, 862-8. https://doi.org/10.1016/S0140-6736(94)92832-0
  10. Creighton TE (1983). Biochemical, physiological, toxicological and clinical aspects, In: A. Larsson, S. Orrenins, A. Holmgren, B Mannervik (Ed.), Function of glutathione, Raven press, New York, pp. 205-213.
  11. Cochrane CG (1991). Cellular injury by oxidants. Am J Med, 91, 23. https://doi.org/10.1016/0002-9343(91)90069-A
  12. Eveson JW (1981). Animal models of intra-oral chemical carcinogenesis: a review. J Oral Pathol, 10, 129-46. https://doi.org/10.1111/j.1600-0714.1981.tb01259.x
  13. Fann YC, Metosh-Dickey CA, Winston GW, (1999). Enzymatic and non-enzymatic production of free radicals from the carcinogens 4-nitroquinoline N-oxide and 4-hydroxylaminoquinoline N-oxide. Chem Res Toxicol, 12, 450-8. https://doi.org/10.1021/tx980238p
  14. Feron VJ, Tie HP, De Vrjer F, et al (1991). Aldehydes: occurrence, carcinogenic potential mechanism of action and risk assessment. Mutat Res, 259, 362-6.
  15. Fields WR, Li Y, Townsend AJ (1994). Protection by transfected glutathione S-transferase isozymes against carcinogen induced alkylation of cellular macromolecules in human MCF-7 cells. Carcinogenesis, 15, 1155-60. https://doi.org/10.1093/carcin/15.6.1155
  16. Fisker AV (1990). Experimental oral carcinogenesis. A basic rat model for the study of oral carcinogenesis using the carcinogen 4-nitroquinoline1-oxide. Dan Med Bull, 37, 433-42.
  17. Goth-Goldstein R, Tincknell BP, Hughes M (1984). Toxicity of 4-nitroquinoline 1-oxide in Chinese hamster ovary cells: influence of cell density and of position in the cell cycle. Mutat Res, 140, 209-13. https://doi.org/10.1016/0165-7992(84)90079-4
  18. Griffith OW (1980). Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem, 106, 207-12. https://doi.org/10.1016/0003-2697(80)90139-6
  19. Gupta SK, Halder N, Srivastava S, et al (2002). Green tea (Camellia sinensis) protects against selenite-induced oxidative stress in experimental cataractogenesis. Ophthalmic Res, 34, 258-63. https://doi.org/10.1159/000063881
  20. Habig WH, Pabst MJ, Jacoby BW (1974). Glutathione S transferase. The first enzymatic step in mercapturic acid formation. J Biol Chem, 249,1730-7.
  21. Halliwell B, Murcia MA, Chirico S, Aruoma OI (1995). Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr, 35, 7-20. https://doi.org/10.1080/10408399509527682
  22. Hendler FJ, Yuan B, Oechlsi MN (1996). 4-NQO oral carcinogenesis, a murine model of human head and neck cancer. Head Neck Cancer, ?, 79-87.
  23. Irshad M, Chaudhuri PS (2002). Oxidant-antioxidant system: Role and significance in human body. Indian J Exp Biol 40, 1233-1239.
  24. Isbrucker RA, Bausch J, Edwards JA, Wolz E (2006). Safety studies on epigallocatechin gallate (EGCG) preparations. Part 1: Genotoxicity. Food Chem Toxicol, 44, 626-35. https://doi.org/10.1016/j.fct.2005.07.005
  25. Ito N (1981). In vivo carcinogenesis of 4-nitroquinoline 1-oxide and related compounds. Carcinog Compr Surv, 6, 117-53
  26. Jankun J, Selman SH, Swiercz R, Skrzypczak-Jankun E (1997). Why drinking green tea could prevent cancer. Nature, 387, 561. https://doi.org/10.1038/42381
  27. Katiyar SK, Mukhtar H (1996). Tea in chemoprevention of cancer; epidemiological and experimental studies. Int J Oncol, 8, 221-38.
  28. Koch CJ, Stobbe CC, Hettiaratchi P (1989). Combined radiation protective and radiation sanitizing agents: IV. Measurement of intracellular protector concentrations. Int J Radiat Oncol Biol Phys, 16, 1025-7. https://doi.org/10.1016/0360-3016(89)90908-5
  29. Misra HP, Fridovich I (1972). The role of superoxide anion in the auto oxidation of epinephrine and a simple assay of superoxide dismutase. J Biol Chem, 247, 3170-5.
  30. Moron MS, Depierre JW, Mannervik B (1979). Levels of glutathione, glutathione reductase and glutathione-Stransferase activities in rat lung and liver. Biochim Biophys Acta, 582, 67-78. https://doi.org/10.1016/0304-4165(79)90289-7
  31. Nunoshiba T, Demple B (1993). Potent intracellular oxidative stress exerted by the carcinogen 4-nitroquinoline-N-oxide. Cancer Res, 53, 3250-2.
  32. Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-8. https://doi.org/10.1016/0003-2697(79)90738-3
  33. Ramotar D, Belanger E, Brodeur I, et al (1998). A yeast homologue of the human phosphotyrosyl phosphatase activator PTPA is implicated in protection against oxidative DNA damage induced by the model carcinogen 4-nitroquinoline 1-oxide. J Biol Chem, 273, 21489-96. https://doi.org/10.1074/jbc.273.34.21489
  34. Ray G, Husian SA (2002). Oxidant, antioxidants and carcinogenesis, Indian J Exp Biol, 40, 1213-2.
  35. Rotruck JT, Pope AL, Ganther HE, et al (1973). Selenium : biochemical rote as a component of Glutathione peroxidase. Science, 179, 588-90. https://doi.org/10.1126/science.179.4073.588
  36. Sedlak J, Lindsay RH (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem, 25, 192-205. https://doi.org/10.1016/0003-2697(68)90092-4
  37. Srinivasan P, Sabitha KE, Shyamaladevi CS (2004). Therapeutic efficacy of green tea polyphenols on cellular thiols in 4-Nitroquinoline 1-oxide induced oral carcinogenesis. Chem Biol Inter, 149, 81-7. https://doi.org/10.1016/j.cbi.2004.06.006
  38. Srinivasan P, Sabitha KE, Shyamaladevi CS (2006). Modulatory efficacy of green tea polyphenols on glycoconjugates and immunological markers in 4-Nitroquinoline 1-oxide-induced oral carcinogenesis-A therapeutic approach. Chem Biol Interact, 162, 149-56 https://doi.org/10.1016/j.cbi.2006.05.021
  39. Srinivasan P, Sabitha KE, Shyamaladevi CS (2007). Attenuation of 4-Nitroquinoline 1-oxide induced in vitro lipid peroxidation by green tea polyphenols. Life Sci, 12, 1080-6.
  40. Staal GEJ, Visser J, Veeger C (1969). Purification and properties of glutathione reductase of human erythrocytes. Biochim Biophys Acta, 185, 39-48. https://doi.org/10.1016/0005-2744(69)90280-0
  41. Takahara S, Hamilton BH, Nell JV, et al (1960). Hypocatalasemia, a new genetic carrier state. J Clin Invest, 29, 610-9.
  42. Tsuda H (1990). Role of DT diaphorase the cytotoxicity of menadione and 4-nitroquinoline-1-oxide in cultured mammalian fibroblastic cells. Cancer Lett, 55, 195-9. https://doi.org/10.1016/0304-3835(90)90119-I
  43. Upsani CD, Khera A, Balaraman R (2001). Effect of Lead and vitamin E, C or spiruline on malondialdehyde, conjucated dienes and hydroperoxides in rats. Ind J Exp Biol, 39, 70-4.
  44. Vaca CE, Wilhelm J, Harms-Rihsdash M (1998). Interaction of lipid peroxidation product with DNA. Mutat Res Rev Genet Toxicol, 195, 137.
  45. Varnes ME, Biaglow JE (1979). Interactions of the carcinogen 4-nitroquinoline 1-oxide with the non-protein thiols of mammalian cells. Cancer Res, 39, 2960-5.
  46. Walker IG, Sridhar R (1976). The formation and repair of singlestrand breaks in DNA of cultured mammalian cells treated with UV-light, methylating agents or 4-nitroquinoline-1- oxide. Chem Biol Interact, 12, 229-39. https://doi.org/10.1016/0009-2797(76)90039-9
  47. Yamamoto K, Inoue S, Kawanishi S (1993). Site-specific DNA damage and 8-hydroxydeoxyguanosine formation by hydroxylamine and 4-hydroxyaminoquinoline 1-oxide in the presence of Cu(II): role of active oxygen species. Carcinogenesis, 14, 1397-401. https://doi.org/10.1093/carcin/14.7.1397
  48. Yu R, Jiao JJ, Duh JL, et al (1997). Activation of mitogenactivated protein kinases by green tea polyphenols: potential signaling pathways in the regulation of antioxidantresponsive element-mediated phase II enzyme gene expression. Carcinogenesis, 18, 451-6. https://doi.org/10.1093/carcin/18.2.451
  49. Zhang Y, Chen SY, Hsu T, Santella RM (2002). Immunohistochemical detection of malondialdehyde-DNA adducts in human oral mucosa cells. Carcinogenesis, 23, 207-11. https://doi.org/10.1093/carcin/23.1.207

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