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Anti-Proliferation Effects of Benzimidazole Derivatives on HCT-116 Colon Cancer and MCF-7 Breast Cancer Cell Lines

  • Al-Douh, Mohammed Hadi (Chemistry Department, Faculty of Science, Hadhramout University of Science and Technology (HUST)) ;
  • Sahib, Hayder B. (Pharmacology Department, College of Pharmacy, Karbala University) ;
  • Osman, Hasnah (School of Chemical Sciences, Universiti Sains Malaysia (USM)) ;
  • Hamid, Shafida Abd (Kulliyyah of Science, International Islamic University Malaysia (IIUM)) ;
  • Salhimi, Salizawati M. (School of Pharmaceutical Sciences, Universiti Sains Malaysia (USM))
  • Published : 2012.08.31

Abstract

Benzimidazoles 1-4 were obtained using modified synthesis methods and studied for their ability to inhibit cell proliferation of colon cancer cell HCT-116 and breast cancer cell MCF-7 using MTT assays. In the HCT-116 cell line, benzimidazole 2 was found to have an $IC_{50}$ value of $16.2{\pm}3.85{\mu}g/mL$ and benzimidazole 1 a value of $28.5{\pm}2.91{\mu}g/mL$, while that for benzimidazole 4 was $24.08{\pm}0.31{\mu}g/mL$. In the MCF-7 cell line, benzimidazole 4 had an $IC_{50}$ value of $8.86{\pm}1.10{\mu}g/mL$, benzimidazole 2 a value of $30.29{\pm}6.39{\mu}g/mL$, and benzimidazole 1 a value of $31.2{\pm}4.49{\mu}g/mL$. Benzimidazole 3 exerted no cytotoxity in either of the cell lines, with $IC_{50}$ values $>50{\mu}g/mL$. The results suggest that benzimidazoles derivatives may have chemotherapeutic potential for treatment of both colon and breast cancers.

Keywords

Benzimidazole;hydroxyl group;benzyloxy group;anti-proliferation;HCT-116;MCF-7;MTT assay

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