Is Short-term Exercise a Therapeutic Tool for Improvement of Cardioprotection Against DOX-induced Cardiotoxicity? An Experimental Controlled Protocol in Rats

  • Ashrafi, Javad (Department of Sport Physiology, College of Physical Education and Sport Sciences, University of Mazandaran) ;
  • Roshan, Valiollah Dabidi (Department of Sport Physiology, College of Physical Education and Sport Sciences, University of Mazandaran)
  • Published : 2012.08.31


Background and Objective: Cardiotoxicity and oxidative stress is a life-threatening side effect of doxorubicin (DOX). We investigate the effects of short-term exercise as therapeutic tool for improvement of cardioprotection against DOX-induced cardiotoxicity in the rat. Methods: Wistar males (weighing $257{\pm}28g$) were divided into six groups: (1) control+placebo (2) control+DOX $^{-1}$ (3) control+DOX $^{-1}$ (4) training+placebo (5) training+ DOX$^{-1}$ (6) training+DOX $^{-1}$. Cardiotoxicity was induced by DOX (10 and $^{-1}$). The rats in groups 4, 5 and 6 experienced treadmill running of 25 to $^{-1}$ and 15 to $17m.min^{-1}$, 5 days/wk for 3 wk. At the end of the endurance training program, rats in the 1 and 4 groups, in the 2 and 5 groups and in the 3 and 6 groups received saline solution, DOX $^{-1}$ and DOX $^{-1}$, respectively. Result: DOX administration (10 and $^{-1}$) caused significant increase in MDA and Apelin, an insignificant increase in NO and a significant decrease in SOD, as compared to the C+P group. Three weeks of the pretreatment endurance exercise resulted in a significant increase of Apelin and SOD, an insignificant increase of NO and an insignificant decrease of MDA, as compared to the C+P group. Furthermore, after three weeks of endurance training and DOX treatment with $^{-1}$ and $^{-1}$, a significant increase in apelin and SOD, and a significant decrease in MDA were detected in comparison to C+DOX10 and/or C+DOX20 groups. There was a significant difference between DOX$^{-1}$ and DOX$^{-1}$ treatments in MDA levels only. Conclusion: Pretreatment exercise may improve myocardial tolerance to DOX-induced cardiotoxicity by inhibition of oxidative stress and up-regulation of antioxidants in heart tissue.


Cardiac toxicity;doxorubicin;oxidative stress;aerobic training;rat model


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