Effect of Dietary Grape Pomace Fermented by Saccharomyces boulardii on the Growth Performance, Nutrient Digestibility and Meat Quality in Finishing Pigs

  • Yan, L. (Department of Animal Resource and Science, Dankook University) ;
  • Kim, I.H. (Department of Animal Resource and Science, Dankook University)
  • Received : 2011.06.20
  • Accepted : 2011.08.04
  • Published : 2011.12.01


Fifty-six [(Duroc${\times}$Yorkshire)${\times}$Landrace] pigs with an average initial BW of 19.3${\pm}$0.17 kg were used in this 15-wk growth experiment to investigate the effects of grape pomace fermented by Saccharomyces boulardii on pig growth performance, nutrient digestibility and quality attributes of pork. Pigs were allotted to 2 dietary treatments (7 replications) based on their initial BW in a randomized complete block design. The experimental treatments were: i) control (CON; basal diet), ii) FGPP (CON+30 g/kg fermented grape pomace product). Dietary FGPP improved (p<0.05) average daily gain (ADG), coefficient apparent total tract digestibility (CATTD) of dry matter (DM) and nitrogen (N) during 35-70 d of the experiment. Similarly, pigs fed the FGPP supplemented diet had a higher N digestibility (p<0.05) in the finisher phase (day 71-105). Dietary FGPP increased (p<0.05) the marbling score, the redness ($a^*$) and yellowness ($b^*$) values, as well as the anti-oxidative ability (lower TBARS). The inclusion of FGPP reduced palmitic acid (C:16:0), stearic acid (C:18:0), arachidic acid (C:20:0) and SFA levels (p<0.05) in subcutaneous fat. An increased (p<0.05) linoleic acid (C18:2n6), total PUFA and PUFA/SFA ratio were observed in the FGPP group. Dietary FGPP supplementation decreased the arachidic acid (C:20:0) level in longissimus muscle (LM). In conclusion, dietary inclusion of FGPP at the level of 30 g/kg improved the growth performance, nutrients digestibility and altered the fatty acid pattern in the subcutaneous fat as well as some attributes of pork meat.


Grape Pomace;Growth Performance;Meat Quality;Pig


  1. AOAC. 2000. Official methods of analysis of AOAC International, 17th edition. Association of Official Analytical Chemistry. Maryland, USA.
  2. Brenes, A., A. Viveros, I. Goni, C. Centeno, S. G. Sayago-Ayerdi, I. Arija and F. Saura-Calixto. 2008. Effect of grape pomace concentrate and vitamin E on digestibility of polyphenols and antioxidant activity in chickens. Poult. Sci. 87:307-316.
  3. Buckenhuskes, H., H. A. Jensen, R. Andersson, A. G. Fernandez and M. Rodrigo. 1990. Fermented vegetables. In: Processing and Quality of Foods in Food Biotechnology: Avenues to Healthy and Nutritious Products (P. Zeuthen, J. C. Cheftel, C. Eriksson, T. R. Gormley, P. Linko and K. Paulus). Vol. 2. Elsevier, London.
  4. Dolara, P., C. Luceri, C. DeFilippo, A. P. Femia, L. Giovannelli, G. Caderni, C. Cecchini, S. Silvi, C. Orpianesi and A. Cresci. 2005. Red wine polyphenols influence carcinogenesis, intestinal microflora, oxidative damage and gene expression profiles of colonic mucosa in F344 rats. Mut. Res./Fundam. Mol. Mech. Muta. 591:237-246.
  5. Endo, J. and M. Nakano. 1999. Influence of a probiotic on productivity, meat components, lipid metabolism, caecal flora and metabolites, and raising environment in broiler production. Anim. Sci. J. 70:207-218.
  6. Frank, J. 2005. Beyond vitamin E supplementation: An alternative strategy to improve vitamin status. J. Plant Physiol. 162:834-843.
  7. Goni, I., N. Martin and F. Saura-Calixto. 2005. In vitro digestibility and intestinal fermentation of grape seed and peel. Food Chem. 90:281-286.
  8. Goni, I., A. Brenes, C. Centeno, A. Viveros, F. Saura-Calixto, A. Rebole, I. ArijaI and R. Estevez. 2007. Effect of dietary grape pomace and vitamin E on growth performance, nutrient digestibility and susceptibility to meat lipid oxidation in chickens. Poult. Sci. 86:508-516.
  9. Huang, R. L., Y. L. Yin, K. P. Wang, T. J. Li and J. X. Liu. 2003. Nutritional value of fermented and not fermented material of distiller's grains in pig nutrition. J. Anim. Feed. Sci. 12:261-269.
  10. Hughes, R. J., J. D. Brooker and C. Smyl. 2005. Growth rate of broiler chickens given condensed tannins extracted from grape seed. Aust. Poult. Sci. Symp. 17:65-68.
  11. Jansman, A. J. M., J. Huisman and A. F. B. van der Poel. 1989. Recent Advances in Research of Antinutritional Factors in Legume Seeds. In: Pudoc, Wagemomgen(Ed. J. Huisman, A. F. B. van der Poel and I. E. Liener), the Netherlands. pp. 176-180.
  12. Kamm, K., S. Hoppe, G. Breves, B. Schroder and M. Schemann. 2004. Effects of the probiotic yeast Saccharomyces boulardi on the neurochemistry of myenteric neurones in pig jejunum Neurogastroenterol. Motil. 16:53-60.
  13. Mathew, A. G., S. E. Chattin, C. M. Robbins and D. A. Golden. 1998. Effects of a direct-fed yeast culture on enteric microbial populations, fermentation acids, and performance of weanling pigs. J. Anim. Sci. 76:2138-2145.
  14. Mancini, R. A. and M. C. Hunt. 2005. Current research in meat color. Meat Sci. 71:100-121.
  15. Mitsumoto, M., R. N. Arnold, D. M. Schaefer and R. G. Cassens. 1993. Dietary versus postmortem supplementation of vitamin E on pigment and lipid stability in ground beef. J. Anim. Sci. 71:1812-1816.
  16. Mielnick, M. B., E. Olsen, G. Vogt, D. Adeline and G. Skrede. 2006. Grape seed extract as antioxidant in cooked, cold stored turkey meat. LWT Food Sci. Technol. 39:191-198.
  17. Monahan, F. L., A. Asghar, J. I. Gray, D. L. Buckley and P. A. Morrissey. 1994. Effect of oxidized dietary lipid and vitamin E on the colour stability of pork chops. Meat Sci. 37:205-215.
  18. Morrissey, P. A., P. J. E. Sheehy, K. Galvin, J. P. Kerry and D. J. Buckley. 1998. Lipid stability in meat and meat products. Meat Sci. 49:S73-S86.
  19. Murrieta, C. M., B. W. Hess and D. C. Rule. 2003. Comparison of acidic and alkaline catalysts for preparation of fatty acid methyl esters from ovine muscle with emphasis on conjugated linoleic acid. Meat Sci. 65:523-529.
  20. NPPC. 1991. Procedures to Evaluate Market Hogs (3rd Ed.). National Pork Producers Council, Des Moines, IA.
  21. NRC. 1988. Nutrient Requirements of Swine (9th Ed.). National Academy Press, Washington, DC.
  22. O'Grady, M. N., R. Carpenter, P. B. Lynch, N. M. O'Brien and J. P. Kerry. 2008. Addition of grape seed extract and bearberry to porcine diets: influence on quality attributes of raw and cooked pork. Meat Sci. 78:438-446.
  23. Papadopoulou, C., K. Soulti and I. G. Roussis. 2005. Potential antimicrobial activity of red and white wine phenolic extracts against strains of Staphylococcus aureus, Escherichia coli and Candida albicans. Food Technol. Biotechnol. 43:41-46.
  24. Pascual, J. V., M. Rafecas, M. A. Canela, J. Boatella, R. Bou, A. C. Barroeta and R. Codony. 2007. Effect of increasing amounts of a linoleic-rich dietary fat on the fat composition of four pig breeds. Part II: Fatty acid composition in muscle and fat tissues. Food Chem. 100:1639-1648.
  25. Pazos, M., J. M. Gallardo, J. P. Torres and I. Medina. 2005. Activity of grape polyphenols as inhibitors of fish lipids and frozen fish muscle. Food Chem. 92:547-557.
  26. Puiggross, F., N. Llopiz, A. Ardevol, C. Blade, L. Arola and M. J. Salvado. 2005. Grape seed proanthocyanidins prevent oxidative injury by modulating expression of antioxidant enzyme system. J. Agric. Food Chem. 53:6080-6086.
  27. SAS. 1996. SAS user's guide. Release 6.12 edition. SAS Institude. Inc Cary NC. USA.
  28. Saura-Calixto, F., A. Garcı´a-Alonso, I. Goñi and L. Bravo. 2000. In vitro determination of the indigestibility fraction of foods: an alternative to dietary fibre analysis. J. Agric. Food Chem. 48:3342-3347.
  29. Sayago-Ayerdi, S. G., A. Brenes, A. Viveros and I. Goni. 2009. Antioxidative effect of dietary grape pomace concentrate on lipid oxidation of chilled and long-term frozen stored chicken patties. Meat Sci. 83:528-533.
  30. Shen, Y. B., X. S. Piao, S. W. Kim, L. Wang, P. Liu, I. Yoon and Y. G. Zhen. 2009. Effects of yeast culture supplementation on growth performance, intestinal health, and immune response of nursery pigs. J. Anim. Sci. 87:2614-2624.
  31. Williams, C. H., D. J. David and O. Iismaa. 1962. The determination of chromic oxide in faeces samples by atomic absorption spectrophotometry. J. Agric. Sci. 59:381-385.
  32. Witte, V. C., G. F. Krause and M. E. Bailey. 1970. A new extraction method for determining 2-thiobarbituric acid values for pork and beef during storage. J. Food Sci. 35:585-592.
  33. Yi, C., J. Shi, J. Kramer, S. Xue., Y. M. Jiang, M. W. Zhang, Y. Ma and J. Pohorly. 2009. Fatty acid composition and phenolic antioxidants of winemaking pomace powder. Food Chem. 114:570-576.

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