DOI QR코드

DOI QR Code

Effects of Squid Ink on Growth Performance, Antioxidant Functions and Immunity in Growing Broiler Chickens

  • Liu, Huazhong ;
  • Luo, Ping ;
  • Chen, Shaohong ;
  • Shang, Jianghua
  • Received : 2011.05.03
  • Accepted : 2011.07.12
  • Published : 2011.12.01

Abstract

This study was conducted to explore the effects of squid ink on growth performance, immune functions and antioxidant ability of broiler chickens during a period of six weeks. Either sex Arbor Acres broilers were equally allotted to 4 groups with 3 replicates of 20 chickens each. Broilers diets for the 4 test groups were prepared separately with starter and finisher phases. Control chickens were fed with basal diet and birds of group Exp 2, Exp 4 and Exp 6 were fed with the basal diet supplemented with 2%, 4% and 6% of squid ink, respectively. Broilers were sacrificed to investigate antioxidant parameters of sera, indices of thymus, spleen and bursa of fabricius and spleen lymphocyte proliferation, as well as growth performance on the $21^{th}$ and $42^{th}$ day. The results revealed that, i) squid ink promoted growth performance of broilers during days 22 to 42 and the whole trial period (p<0.05 or p<0.01); ii) squid ink elevated relative weight of the three immune organs during the starter phase and spleen lymphocyte proliferation throughout the experiment (p<0.05); iii) squid ink increased SOD activity and decreased MDA level in sera from broilers during the whole period (p<0.05). The above results suggest that squid ink could improve growth performance, antioxidant ability and immune functions of growing broiler chickens and be employed in the development of feed additives for animals.

Keywords

Squid Ink;Antioxidant Ability;Immune Function;Growth Performance;Broilers

References

  1. Chen, S. G., C. H. Xue, Y. Xue, Z. J. Li, X. Gao and Q. Ma. 2007. Studies on the free radical scavenging activities of melanin from squid ink. Chin. J. Mar. Drugs. 26(1):24-27.
  2. Chen, X. E. 2000. Study on nutritional composition of Sepialla maindroni ink. J. Zhejiang Ocean Univ. 19(4):324-326.
  3. He, S., G. L. Xie and M. C. He. 2002. Studies of sepia influence on NK cells activity and effect on inhibition tumor in mice. Chin. J. Mar. Drugs. 21(5):37-39.
  4. He, S., G. L. Xie, F. Z. Liu and C. L. Lu. 1999. The study about IL-2 activity induced by sepia in mice. Chin. J. Mar. Drugs. 18(1):11-12.
  5. Liu, C. Y., Q. Teng, X. Y. Zhou and R. L. Tan. 1996. Effects of sepia on erythrocyte's adhesion to tumor cells. Chin. J. Mar. Drugs. 15(3):14-16.
  6. Liu, H. Z., G. Wang, Y. Z. Guo, J. Q. Pan, Y. Huang, J. P. Zhong and K. Li. 2009. Protective effect of squid ink on cyclophosphamide-induced oxidative injury of kidney in mice. Chin. J. Nephrol. 25(10):804-805.
  7. Lu, C. L., S. He, B. X. Liu, L. X. Na, J. Z. Yan and G. Fu. 1999. The immuno-stimulating activations of squid ink and its extracts. Chin. J. Mar. Drugs. 18(2):32-34.
  8. Takaya, Y., H. Uchisawa, H. Matsue, B. Okuzaki, F. Narumi, J. Sasaki and K. Ishida. 1994a. An investigation of the antitumor peptidoglycan fraction from aquid ink. Biol. Pharm. Bull. 17(6):846-849. https://doi.org/10.1248/bpb.17.846
  9. Takaya, Y., H. Uchisawa, K. Hanamatsu, F. Narumi, B. Okuzaki and H. Matsue. 1994b. Novel fucose-rich glycosaminoglycans from squid ink bearing repeating unit of trisaccharide structure (-6GalNAc alpha 1-3GlcA beta 1-3Fuc alpha 1-)n. Biochem. Biophys. Res. Commun. 198(2):560-567. https://doi.org/10.1006/bbrc.1994.1082
  10. Wang, G. 2010a. Study of protective effects sepia ink polysaccharides on chemotherapy. M.D. Thesis, Guangdong Ocean University, Zhanjiang, China.
  11. Wang, G., H. Z. Liu, J. L. Wu, Q. W. Zeng, Y. P. Chen, C. L. Yang and J. P. Zhong. 2010b. Study of sepia ink extract on protection from oxidative damage of cardiac muscle and brain tissue in mice. Chin. JMAP. 27(2):95-98.
  12. Wang, G., Y. Z. Guo, S. B. Guan, J. P. Zhong, J. Q. Pan, Y. Huang and H. Z. Liu. 2009. Protective effect of sepia ink extract on cyclophosphamide-induced pulmonary fibrosis in mice. Chin. J. Mar. Drugs. 28(1):36-40.
  13. Wang, Q., C. L. Lü, C. Lü, J. Liu, Y. M. Cao and J. Z. Yan. 2001. Study on the induction of $IFN-{\gamma}$ and LAK cells activity in mice stimulated with sepia. Chin. J. Mar. Drugs. 20(1):37-39.
  14. Xie, G. L. and S. He. 2001. Study about CSF activity induced by sepia in mice. Chin. J. Mar. Drugs. 20(3):25-27.
  15. Zhang, Y. J., X. Y. Xu and B. Q. Wang. 2003. The influence of compound sepia capsules on SOD activity in mice. Chin. New. Med. 2(1):23-27.
  16. Zheng, G. L., X. Y. Zhang, Y. G. Zhou, Q. C. Meng and W. G. Gong. 2002. Comparison between partial components and trace elements in sepia and squid ink. Chin. J. Mar. Drugs. 21(3):12-14.
  17. Zhong, J. P., G. Wang, J. H. Shang, J. Q. Pan, K. Li, Y. Huang and H. Z. Liu. 2009. Protective effects of squid ink extract towards hemopoietic injuries induced by cyclophosphamide. Mar. Drugs 7(1):9-18. https://doi.org/10.3390/md7010009

Cited by

  1. Effectiveness of treatment of iron deficiency anemia in rats with squid ink melanin–Fe vol.5, pp.1, 2014, https://doi.org/10.1039/C3FO60383K
  2. Dietary squid ink polysaccharides ameliorated the intestinal microflora dysfunction in mice undergoing chemotherapy vol.5, pp.10, 2014, https://doi.org/10.1039/C4FO00408F
  3. Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice vol.5, pp.12, 2014, https://doi.org/10.1039/C4FO00569D
  4. Dietary squid ink polysaccharide induces goblet cells to protect small intestine from chemotherapy induced injury vol.6, pp.3, 2015, https://doi.org/10.1039/C4FO01191K
  5. Effect of Melanin-Free Ink on Mechanical Properties and Yellow Discolouration of Protein Film from Washed Sardine Mince vol.12, pp.2, 2017, https://doi.org/10.1007/s11483-017-9473-8
  6. extract improved growth performance and liver antioxidative function in broilers vol.16, pp.4, 2017, https://doi.org/10.1080/1828051X.2017.1302826
  7. Magellanic penguin telomeres do not shorten with age with increased reproductive effort, investment, and basal corticosterone vol.7, pp.15, 2017, https://doi.org/10.1002/ece3.3128
  8. Effect of dietary pine needles powder supplementation on growth, organ weight and blood biochemical profiles in broilers pp.0974-1844, 2017, https://doi.org/10.1080/09712119.2017.1351977
  9. Cephalopod Ink: Production, Chemistry, Functions and Applications vol.12, pp.5, 2014, https://doi.org/10.3390/md12052700
  10. The effect of melanin-free extract from Sepia esculenta ink on lipid peroxidation, protein oxidation and water-holding capacity of tilapia fillet during cold storage vol.12, pp.1, 2018, https://doi.org/10.1186/s13065-018-0402-9
  11. A Potential Adjuvant Agent of Chemotherapy: Sepia Ink Polysaccharides vol.16, pp.4, 2018, https://doi.org/10.3390/md16040106
  12. ) During Storage vol.116, pp.1755-1315, 2018, https://doi.org/10.1088/1755-1315/116/1/012031