- Volume 24 Issue 11
DOI QR Code
Effect of MEM Vitamins Supplementation of In vitro Maturation Medium and In vitro Culture Medium on the Development of Porcine Embryos
- Kim, J.Y. (Dr. Lee Eun Ji Mari Fertility Clinic) ;
- Lee, E.J. (Dr. Lee Eun Ji Mari Fertility Clinic) ;
- Park, J.M. (Dr. Lee Eun Ji Mari Fertility Clinic) ;
- Park, H.D. (Department of Biotechnology, Daegu University)
- Received : 2011.06.24
- Accepted : 2011.08.29
- Published : 2011.11.01
This study was carried out to examine the influence of minimum essential medium (MEM) vitamins supplementation to in vitro maturation medium and in vitro culture medium on the development of porcine embryos. Porcine embryo development was investigated following cultivation in both in vitro maturation and culture medium with the supplementation of MEM vitamins (0, 0.1, 0.2 and 0.4%) using immature oocytes collected from the ovary of prepubertal gilts. Embryo development was observed and the total cell number in each blastocyst generated under the culture conditions was quantified following supplementation of the medium. The embryonic development rate of the blastocyst and hatched blastocyst was higher, but not significantly so, when 0.4% MEM vitamins were supplemented to the in vitro maturation medium of the porcine oocyte. Interestingly, the total number of cells in the blastocyst was significantly higher in the in vitro maturation MEM vitamins supplemented group compared to either the untreated group or the group which had MEM vitamins supplemented to both in vitro maturation and in vitro culture medium simultaneously (p<0.05). Therefore, the supplementation of 0.4% MEM vitamins to the in vitro mature medium has a beneficial effect on the embryonic development of in vitro produced blastocysts derived from the immature porcine oocytes.
- Abeydeera, L. R. and B. N. Day. 1997. Fertilization and subsequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. Biol. Reprod. 57:729-734. https://doi.org/10.1095/biolreprod57.4.729
- Abeydeera, L. R., W. H. Wang, T. C. Cantley, A. Rieke, C. N. Murphy, R. S. Prather and B. N. Day. 2000. Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology 54:787-797. https://doi.org/10.1016/S0093-691X(00)00390-3
- Abeydeera, L. R., W. H. Wang, R. S. Prather and B. N. Day. 2001. Effect of incubation temperature on vitro maturation of porcine oocytes: Nuclear maturation, fertilization and developmental competence. Zygote 9:331-337.
- Ali, A. A., J. F. Bilodeau and M. A. Sirard. 2003. Antioxidant requirements for bovine oocytes varies during in vitro maturation, fertilization and development. Theriogenology 59:939-949. https://doi.org/10.1016/S0093-691X(02)01125-1
- Camous, S., Y. Heyman, W. Menezo and Y. Menezo. 1984. Cleavage beyong the block stage and survival after transfer of early bovine embyos cultured with trophoblastic vesicles. J. Reprod. Fertil. 72:479-485. https://doi.org/10.1530/jrf.0.0720479
- Chang, H. S., W. T. Cheng, H. K. Wu and K. B. Choo. 2000. Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development. Mol. Reprod. Dev. 56:331-335. https://doi.org/10.1002/1098-2795(200007)56:3<331::AID-MRD1>3.0.CO;2-K
- Choi, Y. H., B. C. Lee, J. M. Lim, S. K. Kang and W. S. Hwang. 2002. Optimization of culture medium for cloned bovine embryos and its influence on pregnancy and delivery outcome. Theriogenology 58:1187-1197. https://doi.org/10.1016/S0093-691X(02)00946-9
- Dobrinsky, J. F., L. A. Johnson and D. Rath. 1996. Development of a culture medium (BECM-3) for porcine embryos: effects of bovine serum albumin and fetal bovine serum on embryo development. Biol. Reprod. 55:1069-1074. https://doi.org/10.1095/biolreprod55.5.1069
- Eyestone, W. H. and N. L. First. 1989. Co-culture of early cattle embryos to the blastocyst stage with oviductal tissue or in conditioned medium. J. Reprod. Fertil. 85:710-720.
- Fukui, Y., Y. Terawaki and H. Ono. 1992. Effect of gonadotropins and steroids and culture on bovine oocytes maturation. Theriogenology 18:161-175.
- Hawthorne, J. N. 1982. Inositol phospholipids. In: Phospholipids (Ed. J. N. Hawthorne and G. B. Ansell). Amsterdam: Elsevier Biomedical Press. 263-278.
- Heyman, Y., Y. Menezo, P. Chesne, S. Camous and V. Garnier. 1987. In vitro cleavage of bovine and ovine early embryos: Improved development using co-culture with trophoblastic vesicles. Theriogenology 27:59-68. https://doi.org/10.1016/0093-691X(87)90070-7
- Hong, J. Y., H. Y. Yong, B. C. Lee, W. S. Hwang, J. M. Lim and S. Lee. 2004. Effects of amino acid on maturation, fertilization and embryo development of pig follicular oocytes in two IVM media. Theriogenology 62:1473-1482. https://doi.org/10.1016/j.theriogenology.2004.02.013
- Iritani, A., K. Niwa and H. Imai. 1978. Sperm peneration of pig follicular oocytes matured in culture. J. Reprod. Fertil. 54:379-383. https://doi.org/10.1530/jrf.0.0540379
- Kamat, J. P. and T. P. Devasagayam. 1996. Methylene blue plus light-induced lipid peroxidation in rat liver microsomes: inhibition by nicotinamide (Vitamin B3) and other antioxidants. Chem. Biol. Interact. 99:1-16. https://doi.org/10.1016/0009-2797(95)03653-9
- Kane, M. T. 1988. The effects of water-soluble vitamins on the expansion of rabbit blastocysts in vitro. J. Exp. Zool. 245:220-223. https://doi.org/10.1002/jez.1402450211
- Kane, M. T. and B. D. Bavister. 1988. Vitamins requirements for development of eight-cell hamster embryos to hatching blastocysts in vitro. Biol. Reprod. 39:1137-1143. https://doi.org/10.1095/biolreprod39.5.1137
- KiKuchi, K., A. Onishi, N. Kashiwazaki, M. Iwamoto, J. Noguchi, H. Kaneko, T. Akita and T. Nagai. 2002. Successful piglet production after transfer of blastocysts produced by a modified in vitro system. Biol. Reprod. 66:1033-1041. https://doi.org/10.1095/biolreprod66.4.1033
- Kitagawa, Y., K. Suzuki, A. Yoneda and T. Watanabe. 2004. Effects of oxygen concentration and antioxidants on the in vitro developmental ability, production of reactive oxygen species (ROS), and DNA fragmentation in porcine embryos. Theriogenology 62:1186-1197. https://doi.org/10.1016/j.theriogenology.2004.01.011
Krishnaswamy, K. and K. J. Sushil. 2004. Effect of vitamin B on oxygen radicals, mitochondrial membrane potential, and lipid peroxidation in
$H_2O_2$-treated U937 monocytes. Free Radic. Biol. Med. 36:423-428. https://doi.org/10.1016/j.freeradbiomed.2003.09.012
- Lane, M. and D. K. Gardner. 1998. Amino acids and vitamins prevent culture-induced metabolic perturbation and associated loss of viability of mouse blastocysts. Hum. Reprod. 13:991-997. https://doi.org/10.1093/humrep/13.4.991
- Legge, M. and M. H. Sellens. 1991. Free radical sacvengers ameliorate the 2-cell block in mouse embryo culture. Hum. Reprod. 6:867-871.
- Lim, J. M., B. C. Reggio, R. A. Godke and W. Hansel. 1997. Perifusion culture system for bovine embryos: improvement of embryo development by use of bovine oviduct epithelial cells, an antioxidant and polyvinyl alcohol. Reprod. Fertil. Dev. 94:411-418.
- Mattioli, M., M. L. Bacci, G. Geleati and E. Seren. 1989. Developemental competence of pig oocytes matured and fertilized in vitro. Theriogenology 31:1201-1207. https://doi.org/10.1016/0093-691X(89)90089-7
- May, J. V., J. P. Frost and D. W. Schomberg. 1988. Differential effects of epidermal growth factor, somatomedian-C/insulinlike growth factor and transforming growth factor-beta on porcine granulose cell DNA synthesis and cell proliferation. Endocrinology 123:168-179. https://doi.org/10.1210/endo-123-1-168
- McKiernen, S. H. and B. D. Bavister. 2000. Culture of one-cell hamster embryos with wate soluble vitamins: pantothenate stimulates blastocyst production. Hum. Reprod. 15:157-164. https://doi.org/10.1093/humrep/15.1.157
- McDowell, L. R. 2000. Vitamins in animal and human nutrition. Ames. IA, Iowa State University Press.
- Mishra, A. and P. B. Seshagiri. 1998. Successful developoment in vitro of hamster 8-cell embryos to 'zona-escaped' and attached blastocysts: assessment of qulaity and trophoblast outgrowth. Reprod. Fertil. Dev. 10:413-420. https://doi.org/10.1071/RD98100
- Motlik, J., N. Grozet and J. Fulka. 1984. Meiotic competence in vitro of pig oocytes isolated from early antral follicles. J. Reprod. Fertil. 72:323-328. https://doi.org/10.1530/jrf.0.0720323
- Murray, R. K., D. K. Granner, P. A. Mayer and V. W. Rodwell. 1990. Lipid peroxidation is a source of free radicals in vitro. Harper's Biochem. 20:142-143.
- Nagai, T., T. Takahashi, H. Masuda, Y. Shioya, M. Kuwayama, M. Fukushima, S. Iwasaki and A. Hanade. 1988. In vitro fertilization of pig oocytes by frozen boar spermatozoa. J. Reprod. Fertil. 84:585-591. https://doi.org/10.1530/jrf.0.0840585
- Ogawa, T., R. P. Ono and T. Marrss. 1987. The effect of serum fractions on single-cell mouse embryos in vitro. J. In vitro Fertil. Embryo. Transfer. 4:153-158. https://doi.org/10.1007/BF01555462
- Olson, S. E. and Jr. G. E. Seidel. 2000. Culture of in vitroproduced bovine embryos with vitamin E improves development in vitro and after transfer to recipients. Biol. Reprod. 62:248-252. https://doi.org/10.1095/biolreprod62.2.248
- Prather, R. and B. N. Day. 1998. Practical consideration for the in vitro production of pig embryos. Theriogenology 49:23-32. https://doi.org/10.1016/S0093-691X(97)00399-3
- Petters, R. M. and K. D. Wells. 1993. Culture of pig embryos. J. Reprod. Fertil. Suppl. 48:61-73.
- Rizos, D. A., S. Glutierrez-Adan, J. Perez-Garnels, M. De La Fuente, P. P. Boland and P. Lonergan. 2003. Bovine embryos culture in the presence or absence of serum implication for blastocyst development, cryotolerance, and messenger RNA expression. Biol. Reprod. 68:236-243.
- Saito, H., T. Berger, D. R. Mishell Jr and R. P. Marrs. 1984. The effect of serum fractions on embryo growth. Fertil. Steril. 41:761-765.
- Stokes, P. J., L. R. Abeydeera and H. J. Leese. 2005. Development of porcine embryos in vivo and in vitro; evidence for embryo 'cross talk' in vitro. Dev. Biol. 284:62-71. https://doi.org/10.1016/j.ydbio.2005.05.001
- Stryer, L. 1988. Biochemistry. New York: W. H. Freeman. 322-325.
- Vance, D. E. 1986. Phospholipid metabolism in eukaryotes. In: Biochemistry of lipids and membranes (Ed. J. E. Vance). Menlo Park, CA: The Benjamin/Cummings Publishing Co. Inc. 242-270.
- Wang, W. H., M. Hosoe and Y. Shioya. 1997. Induction of cortical granule exocytosis of pig oocytes by spermatozoa during meiotic maturation. J. Reprod. Fertil. 109:247-255. https://doi.org/10.1530/jrf.0.1090247
- Xu, X. J., Q. Z. Yang, R. C. Qian and Z. Nakahara. 1992. Development of in vitro fertilized bovine ova in two co-culture systems. Anim. Reprod. 1:372-374.
- Yoshioka, K., C. Suzuki, A. Tanaka, I. M. Anas and S. Iwamura. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66:112-119. https://doi.org/10.1095/biolreprod66.1.112