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Association of Polymorphisms in Fecundity Genes of GDF9, BMP15 and BMP15-1B with Litter Size in Iranian Baluchi Sheep

  • Moradband, F. (Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Sciences, Faculty of Animal and Aquatic Sciences, Sari Agricultural Sciences and Natural, Resources University) ;
  • Rahimi, G. (Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Sciences, Faculty of Animal and Aquatic Sciences, Sari Agricultural Sciences and Natural, Resources University) ;
  • Gholizadeh, M. (Laboratory for Molecular Genetics and Animal Biotechnology, Department of Animal Sciences, Faculty of Animal and Aquatic Sciences, Sari Agricultural Sciences and Natural, Resources University)
  • Received : 2010.11.23
  • Accepted : 2011.04.08
  • Published : 2011.09.01

Abstract

The incidence of mutation in three loci of GDF9, BMP15 and BMP15-1B and their effects on litter sizes was evaluated in Baluchi sheep. Wild-type alleles were detected for BMP15 and BMP15-1B loci and all individuals were found to be as non-carriers for FecB and $FecX^G$ mutations but, a G to A nucleotide substitution was found in GDF9 locus. The frequency of $FecG^+$ (0.82) wild type allele was higher than the frequency of $FecG^l$ (0.18) mutant allele and the frequencies of $FecG^+/FecG^+$, $FecG^+/FecG^1$ and $FecG^1/FecG^1$ genotypes were 0.72, 0.20 and 0.08, respectively in GDF9 locus. The heterozygous ($FecG^+/FecG^1$) and homozygous ($FecG^+/FecG^+$) non-carrier ewes had 0.35 and 0.21 more lambs than the homozygous ($FecG^1/FecG^1$) carrier ewes, respectively (p<0.05). In addition to the finding of segregation of non-additive gene effect on litter size in the previous study in Baluchi sheep, these findings for the first time shows that the $FecG^1$ gene has a major effect on litter size in this breed.

Keywords

BMP15;BMP15-1B;GDF9;Litter Size;Baluchi Sheep

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