Studies on Functional Properties of Mulberry Leaf Extracts and Quality Characteristics of Mulberry Leaf Muffins

뽕잎 항산화능 및 뽕잎가루 머핀의 품질특성

Lee, Hye-Yeon;Jung, Hyeon-A;Kim, Dong-Han;Kwon, Hoo-Ja;Lee, Myung-Hee;Kim, An-Na;Park, Chan-Sung;Yang, Kyung-Mi;Bae, Hyun-Joo

  • Published : 2011.08.30


The function of mulberry leaves and the quality of muffins including mulberry leaves were examined. The electron donating ability of both a water and ethanol extract was 40% for both at 100 ppm, 62 and 72% at 500 ppm, and 77 and 83% at 1,000 ppm respectively. These experiments showed good oxidized substance activity. The SOD-like ability of the water and ethanol extracts at 1,000, 500, and 300 ppm was 49% and 55%, 33% and 39%, and 28% and 33% respectively. These results show that the ethanol extract had higher SOD-like ability than that of the water extract. The nitrite scavenging abilities of 1,000 ppm of the mulberry leaf water extract and ethanol extract at pHs of 1.2 and 3.0 were 40.5 and 47%, and 20 and 22% respectively. The Hunter lightness value tended to be lower as the amount of added mulberry leaves increased compared to the contrast set, so the lightness level with a 7% addition was the lowest. The contrast set was the highest for redness and added mulberry leaves tended to decrease redness at 3% but tended to increase it again with additions >3%. The b-value (yellowness) showed a similar tendency as redness. Adding Mulberry leaves tended to decrease the contrast set to the lowest level, then values increased slowly to a peak at 5% added mulberry leaf and then decreased thereafter. Strength and hardness were less after increasing added mulberry leaf. Cohesiveness, springiness, gumminess and brittleness, decreased at up to 5% added mulberry leaves but the 7% addition showed the same level as the contrast set. The mulberry leaves muffins were less preferred to the contrast set in appearance and color. However, the mulberry leaf muffins were preferred to the contrast set in taste and flavor but the result was not significant. Overall the quality of the muffins with 3% added mulberry leaves was preferred over the 1% added mulberry leaf muffins.


mulberry leaf;mulberry leaf muffins;mulberry leaf powder;mulberry leaf extract;muffin


  1. Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature 26:1198
  2. Choi JH, Oh SK. 1983. Studies on the anti-aging action of Korean ginseng. Korean J Food Nutr 12(4): 323-335
  3. Chae JY, Lee JY, Hong IS, Whangbo D, Choi PW, Lee WC, Kim JW, Choi SW, Ree SJ. 2003. Analysis of functional components of leaves of different mulberry cultivars. J Korean Soc Food Sci Nutr 32:15-21
  4. Choi JL, Jung MA, Jung SH. 2006. Antimicrobial effect of mulberry leaves extracts against oral microorganism. J Dental Hyg Sci 6(4):251-254
  5. Hong HD, Kang NK, Kim SS. 1998. Superoxide dismutase-like activity of apple juice mixed with some fruits and vegetables. Korean J Food Sci Technol 30(6) :1484-1487
  6. Han MR, Kim MH. 2006. Effects of alkaline ionic water and grapefruit seed extracts added immersion solutions on storage characteristics of mulberry leaf soybean curd. J Korean Soc Appl Biol Chem 49(2):108-113
  7. Kang YS, Hong JS. 2009. Quality characteristics of injeulmi made with different ratios of mulberry leaf powder. Korean J Food Cookery Sci 25(3):275-282
  8. Kim DS, Ahn BW, Yeum DM, Lee DH, Kim SB, Park YH 1987. Degradation of carcinogenic nitrosamine formation factor by natural food components. : Nitrite-scavenging effects of vegetable extracts. Bull Korean Fish Soc 20(5): 463-468
  9. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidative activity and physiological activity of some Korean medicinal plants. Korea J Food Sci Technol 27(1):80-85
  10. Kim SJ, Han DS, Moon KD, Rhee JS. 1995. Measurement of superoxide dismutase -like activity of natural antioxidants. Biosci Biotech Biochem 59(2):822-82
  11. Kim SY, Lee WC, Kim HB, Kim AJ, Kim SK. 1998. Antihyperlipidemic effect of methanol extracts from mulberry leaves in cholesterol-induced hyperlidemia rats. J Korean Soc Food Sci Nutr 27:1217-1222
  12. Kim HB, Choung WY, Ryu KS. 1999. Sensory characteristics and blood glucose lowering effect of ice - cream containing mulberry leaf powder. Korea J Seric Sci Technol. 41(3):129-134
  13. Kim SM, Cho YS, Sung SK. 2001. The antioxidant ability and nitrate scavenging ability of plant extract. Korean J Food Sci Technol 33(5):626-632
  14. Kim DW, Kim YH. 2003. Quality characteristics of bread added Monascus anka powder. Korean J Culinary Res 9:39-50
  15. Kim YS, Choi HS, Woo IA, Song TH. 2004. The effect on sensory and mechanical characteristics of functional muffin using Glycyrrhrizae radix extract. Korean J Food Cookery Sci 20(1):95-99
  16. Kim HB. 2005. Anti-oxidative capacity analysis of water-soluble substances according to varieties and maturity stages in mulberry leaves and fruits. Korea J Seric Sci Technol 47(2):62-67
  17. Kim AJ, Kim MH, Han MR. 2005. Effects of mulberry leaf powder supplementation on lead status and minerals content in Pb-administered rats. Korea J Food Sci Technol 38(5):380-385
  18. Kim AJ, Kim SY, Choi MK, Kim MH, Han MR, Chung KS. 2005. Effects of mulberry leaves powder on lipid metabolism in high cholesterol-fed rats. Korea J Food Sci Technol 37(4):636-641
  19. Kim AJ, Yuh CS, Bang IS, Woo KJ. 2005. Study on preparation and quality of jelly using mulberry leaf powder. Korean J Food Cookery Sci 22(1):56-61
  20. Marklund S, Marklund G. 1974. Involvement of superoxide anion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismmutase. Eur J Biochem 47:469-474.
  21. Park SH, Lim SI. 2007. Quality characteristics of muffin added red yeast rice flour. Korean J Food Cookery Sci 39(3):272-275
  22. Shin SM, La SH, Choi MK. 2007. A study on the quality characteristics of Kimchi with mulberry leaf powder. Korean J Food Nutr 20(1):53-62
  23. Yeo SG, Ahn CW, Lee YW, Lee TG, Park YH, Kim SB. 1995. Antioxidative effect of tea extracts from green tea, oolong tea and black tea. J Korean Soc Nutr 24(2):299-304
  24. Ye EJ. 2009. Analyzation and assessment of physiological activity on active ingredient in manufacturing fermented mulberry leaf tea. Doctorate thesis. Daegu Haany University of Korea. pp.53

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