Effects of Sound Stress on Physiological Processes of the American Leafminer, Liriomyza trifolii, and Proteomic Analysis

스트레스 음파 처리에 따른 아메리카잎굴파리(Liriomyza trifolii)의 생리 변화와 프로테오믹 분석

  • Park, Jung-A (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Surakasi, Venkara Prasad (School of Bioresource Science, College of Natural Science, Andong National University) ;
  • Kim, Yong-Gun (School of Bioresource Science, College of Natural Science, Andong National University)
  • 박정아 (안동대학교 자연과학대학 생명자원과학과) ;
  • ;
  • 김용균 (안동대학교 자연과학대학 생명자원과학과)
  • Received : 2011.04.13
  • Accepted : 2011.06.19
  • Published : 2011.06.30


This study investigated the adverse effects of sound treatment on physiological processes of the American leafminer, Liriomyza trifolii, during several developmental stages. Larval feeding activity was analyzed by measuring feeding tunnel length. It was significantly suppressed by sound treatment (5,000 Hz, 95 dB). Sound treatment delayed the pupal period at 315 - 5,000 Hz and prevented adult emergence at 1,000 - 5,000 Hz. Female oviposition was also inhibited by the stress sound treatments. However, phototactic adult movement was not affected by sound treatment. Pupae treated with 5,000 Hz showed marked changes in protein patterns analyzed by two dimensional electrophoresis. MALDI-TOF analysis of specific protein spots indicated that trafficking protein particle complex I, triosephosphate isomerase, hypothetical protein TcasGA2_TC013388, polycystin-2, paraneoplastic neuronal antigen MA1, and tropomyosin I (isoform M) were predicted in the control insects and disappeared in the insects treated with sound. By contrast, DOCK9, cytoskeletal keratin II, and F0F1-ATP synthase beta subunit were predicted only in the sound-treated insects. Furthermore, stress sound significantly increased the susceptibility of L. trifolii to insecticides. These results suggest that physiological processes of L. trifolii are altered by sound stress, which may be exploited to develop a novel physical control tactic against L. trifolii.


Liriomyza trifolii;stress sound;feeding behavior;phototaxis;insecticide


  1. Bradford, M.M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 71: 248-254.
  2. Cai, Y., Y. Maeda, A. Cedzich, V.E. Torres, G. Wu, T. Hayashi, T. Mochizuki, J.H. Park, R. Witzgall and S. Somlo. 1999. Identification and characterization of polycystin-2, the PKD2 gene product. J. Biol. Chem. 274: 28557-28565.
  3. Chandler, L.D. and F.E. Gilstrap. 1986. Within-plant larva distribution of Liriomyza trifolii Burgess (Diptera: Agromyzidae) on Bell peppers. Environ. Entomol. 15: 96-99.
  4. Chen, H.C. and S.R. D'Mello. 2010. Induction of neuronal cell death by paraneoplastic Ma1 antigen. J. Neurosci. Res. 88: 3508-3519.
  5. Furguson, J.S. 2004. Development and stability of insecticide resistance in the leafminer Liriomyza trifolii (Diptera: Agromyzidae) to cyromazine, abamectin, and spinosad. J. Econ. Entomol. 97: 112-119.
  6. Han, M.J., S.H. Lee, J.Y. Choi, S.B. Ahn and M.H. Lee. 1996. Newly introduced insect pest, american serpentine leafminer, Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) in Korea. Kor. J. Appl. Entomol. 35: 309-314.
  7. Hoffmeister, H., A.R. Gallagher, A. Rascle and R. Witzgall. 2010. The human polycystin-2 protein represents an integral membrane protein with six membrane-spanning domains and intracellular N- and C-termini. Biochem. J. 433: 285-294.
  8. Kamimura, M. and M. Kiuchi. 2002. Applying fenoxycarb at the penultimate instar triggers an additional ecdysteroid surge and induces perfect extra larval molting in the silkworm. Gen. Comp. Endocrinol. 128: 231-237.
  9. Kim, J.H., Y.W. Byoun, G.S. Lee and H.Y. Kim. 2007. Evaluation of biological control of Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) using Diglyphus isaea (Walker) (Hymenoptera: Eulophidae) in three seasonal culture types of tomato greenhouse. Kor. J. Appl. Entomol. 46: 71-78.
  10. Kim, Y., D. Kim and J. Lee. 2000. Disturbance of adult eclosion by fenoxycarb, a juvenile hormone mimic, in the beet armyworm, Spodoptera exigua. J. Asia Pac. Entomol. 3: 103-111.
  11. Ku, N.O., D.M. Toivola, P. Strand and M.B. Omary. 2010. Cytoskeletal keratin glycosylation protects epithelial tissue from injury. Nat. Cell Biol. 12: 876-885.
  12. Lim, J.R., H.C. Moon, S.U. Choi, J.H. Kim, K.K. Lee, B.R. Ko, J.S. Choi, Y.K. Jeon and C.Y. Hwang. 2007. Economic injury levels of Liriomyza trifolii Burgess (Diptera: Agromyzidae) infesting eggplant in greenhouse. Kor. J. Appl. Entomol. 46: 409-414.
  13. Liu, X., Y. Wang, H. Zhu, Q. Zhang, X. Xing, B. Wu, L. Song and L. Fan. 2010. Interaction of sedlin with PAM14. J. Cell Biochem. 109: 1129-1133.
  14. Montpetit, B. and E. Conibear. 2009. Identification of the novel TRAPP associated protein Tca17. Traffic. 10: 713-723.
  15. Moon, H.C., J.S. Choi and C.Y. Hwang. 2002. Ovipositional characteristics of Hemiptarsenus zilahisebessi (Hymenoptera: Eulophidae), ectoparasitoid of Liriomyza trifolii (Diptera: Agromyzidae). Kor. J. Appl. Entomol. 41: 21-25.
  16. Park, J.D., Y.S. Ku, D.S. Choi and S.S. Kim. 2001. Damaged aspects, seasonal fluctuations, and attractivity of various colors on Liriomyza trifolii Burgess (Diptera: Agromyzidae) in Gerbera. Kor. J. Appl. Entomol. 40: 97-103.
  17. Park, J.D., H.B. Lee, S.G. Kim, D.I. Kim, I.J. Park, S.C. Kim and K.C. Kim. 2000. Population dynamics and injuries by Liriomyza trifolii (Burgess) in chrysanthemum field. Kor. J. Appl. Entomol. 39: 141-147.
  18. Parrella, M.P. 1987. Biology of Liriomyza. Annu. Rev. Entomol. 32: 201-224.
  19. Parrella, M.P. and C.B. Keil. 1984. Insect pest management: the lesson of Liriomyza. Annu. Rev. Entomol. 32: 201-224.
  20. SAS Institute. 1989. SAS/STAT User's Guide, Release 6.03, Ed. Cary, NC, USA.
  21. Shin, H.J., M.J. Seo and Y.N. Youn. 2006. Ultrastructure of antennal sensilla on american leafminer, Liriomyza trifolii (Diptera: Agromyzidae). Kor. J. Appl. Entomol. 45: 119-129.
  22. Singh, V.B., K.C. Corley, T.H. Phan and M.C. Boadle-Biber. 1990. Increases in the activity of tryptophan hydroxylase from rat cortex and midbrain in response to acute or repeated sound stress are blocked by adrenalectomy and restored by dexamethasone treatment. Brain Res. 516: 66-76.
  23. Trumble, J.T. 1990. Vegetable insect control with minimal use of insecticides. Hortscience 25: 159-163.
  24. Trumble, J.T., I.P. Ting and L. Bates. 1985. Analysis of physiological growth and yield response of celery to Liriomyza trifolii. Entomol. Exp. Appl. 38: 15-21.
  25. Wei, J., L. Zou, R. Kuang and L. He. 2000. Influence of leaf tissue structure on host feeding selection by pea leafminer Liriomyza huidobrensis (Diptera: Agromyzidae). Zool. Studies 39: 295-300.
  26. Yang, J., Z. Zhang, S.M. Roe, C.J. Marshall and D. Barford. 2009. Activation of Rho GTPases by DOCK exchange factors is mediated by a nucleotide sensor. Science 325: 1398-1402.

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